Table 2 Substrate consumption and product formation parameters during anaerobic batch fermentation of a mixture of glucose, arabinose and xylose in defined mineral medium
TMB3075 (xylose reductase/xylitol dehydrogenase) | TMB3076 (xylose isomerase) | |
|---|---|---|
Consumed arabinose g l-1 | 4.04 ± 0.33 | 1.65 ± 0.01 |
Consumed xylose g l-1 | 20.1 ± 0.2 | 4.9 ± 0.7 |
q arabinose* (g arabinose) (g cells)-1 h-1 | 0.014 ± 0.001 | 0.005 ± 0.001 |
q xylose* (g xylose) (g cells)-1 h-1 | 0.09 ± 0.001 | 0.02 ± 0.001 |
Final ethanol titre g l-1 | 14.7 ± 0.5 | 11.8 ± 0.3 |
q ethanol* (g ethanol) (g cells)-1 h-1 | 0.024 ± 0.001 | 0.010 ± 0.001 |
Y ethanol, on total added sugars (g ethanol) (g sugar)-1 | 0.23 ± 0.01 | 0.18 ± 0.01 |
Y ethanol, on consumed sugars (g ethanol) (g sugar)-1 | 0.32 ± 0.01 | 0.41 ± 0.01 |
Y xylitol (g xylitol) (g consumed xylose)-1 | 0.27 ± 0.04 | 0.04 ± 0.02 |
Y arabitol (g arabitol) (g consumed arabinose)-1 | 0.87 ± 0.03 | ND |
Y biomass, on total added sugars (g dry cell weight) (g sugar) -1 | 0.03 ± 0.01 | 0.03 ± 0.01 |
Y biomass, on consumed sugars (g dry cell weight) (g sugar) -1 | 0.04 ± 0.01 | 0.07 ± 0.01 |
Y glycerol (g acetate) (g consumed arabinose)-1 | 0.08 ± 0.001 | 0.09 ± 0.01 |
Y acetate (g acetate) (g consumed arabinose)-1 | 0.020 ± 0.002 | 0.016 ± 0.001 |