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Table 2 Anaerobic co-fermentation on 20 g L-1 glucose, 20 g L-1 xylose and 20 g L-1 arabinose on defined medium

From: Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering

Strains

q(consumption rate) in g (g cell h)-1

g sugar left at 120 h

Y arabitol (g arabitol. g consumed arabinose-1)

Y(g. g consumed xylose-1)

 

Xylose

Arabinose

Xylose

Arabinose

 

Y xylitol

Y glycerol

Y acetate

Y ethanol

TMB3400

0.07 ± 0.00

0.01 ± 0.01

0.23 ± 0.06

18.40 ± 0.12

0.94 ± 0.02

0.42 ± 0.01

0.09 ± 0.01

0.02 ± 0.00

0.22 ± 0.01

TMB3063

0.06 ± 0.02

0.05 ± 0.02

9.71 ± 1.35

13.97 ± 1.59

1.03 ± 0.08

0.21 ± 0.01

0.03 ± 0.00

0.10 ± 0.09

0.24 ± 0.03

TMB3130

0.09 ± 0.02

0.10 ± 0.04

2.11 ± 0.22

1.61 ± 2.28

0.95 ± 0.05

0.31 ± 0.01

0.04 ± 0.00

0.08 ± 0.02

0.29 ± 0.12

  1. The consumption rates of xylose and arabinose as well as the yields of arabitol, xylitol, glycerol, ethanol and acetate were calculated from the pentose phase after glucose depletion. The cell dry weight at the end of fermentation was used for the calculation of the specific consumption rates. TMB3400 only harbours the heterologous xylose-utilizing pathway; TMB3063 is a derivative from TMB3061 (that contains the xylose and arabinose pathways) with extra-copies of AraA gene; TMB3130 is evolved from TMB3061.