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Table 3 Xylose reductase (XR) and xylitol dehydrogenase (XDH) activity (U.mg protein- 1) of crude protein extracts from cells grown in defined medium with 20 g L-1 glucose

From: Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering

Strain

XR activity

U mg-1protein

XDH activity

U mg-1protein

TMB 3400

0.09 ± 0.00 (*)

0.59 ± 0.08 (*)

TMB3061

0.13 ± 0.01 (*)

0.52 ± 0.03 (*)

TMB 3063

0.10 ± 0.01 (*)

0.81 ± 0.08 (*)

TMB 3130

0.72 ± 0.06

1.85 ± 0.61

  1. *Taken from [10]
  2. TMB3400 only harbours the heterologous xylose-utilizing pathway; TMB3063 is a derivative from TMB3061 (that contains the xylose and arabinose pathways) with extra-copies of AraA gene; TMB3130 is evolved from TMB3061.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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