|  | Optimized enzyme proportions (%) | Xyl yield (%) | ||||||
---|---|---|---|---|---|---|---|---|---|
Feedstock | Pretreatment | CBH1 | BG | EG1 | BX | EX3 | CBH2 | MP | Exptl. |
Corn stover | AFEX | 7 | 4 | 20 | 26 | 39 | 4 | 28.2 | 27.8 ± 0.7 |
 | 0.25% NaOH | 23 | 4 | 23 | 23 | 23 | 4 | 29.0 | 30.1 ± 0.5 |
 | Alk. peroxide | 4 | 4 | 30 | 35 | 23 | 4 | 40.4 | 40.0 ± 2.0 |
Switchgrass | AFEX | 4 | 4 | 18 | 23 | 47 | 4 | 26.2 | 24.8 ± 0.5 |
 | 0.25% NaOH | 17 | 4 | 23 | 31 | 21 | 4 | 27.0 | 26.3 ± 0.6 |
 | Alk. peroxide | 4 | 4 | 4 | 37 | 47 | 4 | 40.5 | 39.5 ± 0.3 |
Miscanthus | AFEX | 14 | 4 | 20 | 30 | 28 | 4 | 37.2 | 35.5 ± 1.0 |
 | 0.25% NaOH | 20 | 4 | 28 | 25 | 19 | 4 | 22.6 | 23.0 ± 0.0 |
 | Alk. peroxide | 8 | 4 | 24 | 29 | 31 | 4 | 42.3 | 40.6 ± 0.9 |
DDGS | AFEX | - | - | - | - | - | - | - | < 7.0 |
 | 0.25% NaOH | - | - | - | - | - | - | - | < 7.0 |
 | Alk. peroxide | - | - | - | - | - | - | - | < 7.0 |
Poplar | AFEX | 4 | 4 | 11 | 28 | 47 | 5 | 19.6 | 21.0 ± 0.0 |
 | 0.25% NaOH | 7 | 4 | 25 | 16 | 43 | 4 | 23.0 | 23.8 ± 1.1 |
 | Alk. peroxide | 4 | 4 | 23 | 19 | 46 | 4 | 25.2 | 26.0 ± 0.5 |