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Table 9 Identified proteases and spectrum counts on 1, 3, 7, and 14 days

From: A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

  

Spectrum countc

 

Accession numbera

GH familya

Identified proteinsa

MW (kDa)b

Day 1

Day 3

Day 7

Day 14

SignalPd

AN7962

 

Penicillolysin/deuterolysin metalloprotease

37

170

232

302

318

Y

AN5558

 

Alkaline protease

42

18

194

65

67

Y

AN2903

 

Vacuolar aspartyl protease (protienase A)

43

0

41

51

58

Y

AN7159

 

Hypothetical tripeptidyl-peptidase

71

21

0

0

0

Y

AN10030

 

Hypothetical serine protease

50

0

12

18

31

Y

AN2366

 

Hypothetical serine protease

25

11

12

9

6

Y

AN8445

 

Putative aminopeptidases

54

40

72

74

69

Y

AN1426

 

Serine carboxypeptidase

62

10

52

51

31

Y

AN7231

 

Serine carboxypeptidase

57

8

16

9

15

Y

AN0224

 

Membrane dipeptidase

46

7

9

23

32

Ye

AN2572

 

Dipetidyl dipeptidase

79

9

0

43

91

Y

  1. aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23].
  2. bHypothetical molecular weight of the proteins.
  3. cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
  4. dSignalP was used to predict secretion signals [23, 24].
  5. eSignalP as reported at [25].