Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants

Table 1 Plant lines and primers used for genotyping

Mutant Line	Gene Locus	Insertion line	Primer sequence
irx7	At2g28110	SALK_120296	5’-CGTCATTGATGAGTGATCGTG-3’ fwd
irx7	At2g28110	SALK_120296	5’- TGCAAAATTTGTCATTGTCAC-3’ rev
irx8-6	At5g54690	SALK_008642	5’-TCAAGAAAAATCATCAACCGG-3’fwd
irx8-6	At5g54690	SALK_008642	5’-AACAGCATTAGACCCGAAACC-3’ rev
irx9-2	At2g37090	SALK_057033	5’- GCTGGTAAGGCCTCATTTTTC-3’ fwd
irx9-2	At2g37090	SALK_057033	5’- AACTTACCAACCCACCCATTC-3’ rev

The primers were used to identify transformants that were homozygous for the T-DNA insertions yielding the irx9-2, irx7 and irx8 mutants. Primer sets of right- and left-border primers (RP+LP) specific for each T-DNA insertion were generated from the SIGnAL T-DNA Primer Design website (http://signal.salk.edu/tdnaprimers.2.html). T-DNA left border primers used were LBa1 (5’-TGGTTCACGTAGTGGGCCATCG-3’) and LBb1.3 (5’-ATTTTGCCGATTTCGGAAC-3’).

ISSN: 2731-3654