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Table 1 Effect of glycosylation on GOOX activity on cello- and xylo-oligosaccharides

From: Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictumand variants with reduced substrate inhibition

 

Specific activity of GOOX (μmol min-1mg-1)*

Substrate

Deglycosylated

Glycosylated

Cellobiose

9.03 ± 0.05

9.20 ± 0.11

Xylobiose

12.55 ± 0.11

12.72 ± 0.13

Cellopentaose

8.19 ± 0.03

8.29 ± 0.13

Xylopentaose

11.91 ± 0.09

11.93 ± 0.01

  1. *Specific activity (μmol min-1 mg-1) of deglycosylated and glycosylated GOOX was measured at 37°C for 15 min using 0.5 mM of each substrate.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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