GOOX | GOOX-VN (A38V-S388N) | GOOX-V (A38V) | |||||||
---|---|---|---|---|---|---|---|---|---|
kcat(min-1) | Km(mM) | kcat/Km(mM-1min-1) | kcat(min-1) | Km(mM) | kcat/Km(mM-1min-1) | kcat(min-1) | Km(mM) | kcat/Km(mM-1min-1) | |
Glucose | 378 ± 5* | 15 ± 1 | 25 ± 2 | 361 ± 5 | 15 ± 1 | 24 ± 2 | 625 ± 12 | 12 ± 1 | 50 ± 5 |
Cellobiose | 420 ± 20 | 0.04 ± 0.01 | 9,400 ± 2,000 | 372 ± 14 | 0.05 ± 0.01 | 7,000 ± 1,000 | 719 ± 8 | 0.039 ± 0.002 | 18,400 ± 900 |
Cellotriose | 412 ± 16 | 0.05 ± 0.01 | 8,600 ± 1,000 | 436 ± 14 | 0.08 ± 0.01 | 5,600 ± 600 | 820 ± 50 | 0.05 ± 0.01 | 15,000 ± 3,000 |
Cellotetraose | 595 ± 16 | 0.07 ± 0.01 | 9,000 ± 800 | 470 ± 12 | 0.09 ± 0.01 | 5,200 ± 500 | 770 ± 20 | 0.06 ± 0.01 | 14,000 ± 2,000 |
Cellopentaose | 498 ± 19 | 0.06 ± 0.01 | 8,700 ± 1,100 | 453 ± 14 | 0.09 ± 0.01 | 4,700 ± 400 | 760 ± 30 | 0.057 ± 0.007 | 13,000 ± 2,000 |
Cellohexaose | 430 ± 30 | 0.08 ± 0.02 | 5,500 ± 1,200 | 393 ± 15 | 0.10 ± 0.01 | 4,000 ± 400 | 790 ± 30 | 0.08 ± 0.01 | 9,000 ± 1,000 |
Xylose | 444 ± 12 | 129 ± 8 | 3.4 ± 0.2 | 330 ± 8 | 118 ± 7 | 2.8 ± 0.2 | 741 ± 15 | 118 ± 7 | 6.3 ± 0.4 |
Xylobiose | 522 ± 8 | 0.057 ± 0.003 | 9,100 ± 500 | 449 ± 5 | 0.099 ± 0.005 | 4,500 ± 200 | 890 ± 20 | 0.05 ± 0.01 | 18,000 ± 2,000 |
Xylotriose | 620 ± 14 | 0.08 ± 0.01 | 8,200 ± 700 | 524 ± 10 | 0.10 ± 0.01 | 5,100 ± 400 | 870 ± 30 | 0.08 ± 0.01 | 11,000 ± 1,000 |
Xylotetraose | 583 ± 14 | 0.07 ± 0.01 | 8,200 ± 800 | 488 ± 7 | 0.11 ± 0.01 | 4,400 ± 300 | 960 ± 30 | 0.067 ± 0.007 | 14,000 ± 1,000 |
Xylopentaose | 623 ± 18 | 0.05 ± 0.01 | 11,800 ± 1,400 | 529 ± 7 | 0.104 ± 0.005 | 5,100 ± 200 | 910 ± 30 | 0.05 ± 0.01 | 17,000 ± 2,000 |
Xylohexaose | 516 ± 18 | 0.06 ± 0.01 | 8,100 ± 1,200 | 465 ± 7 | 0.10 ± 0.01 | 4,400 ± 300 | 910 ± 20 | 0.050 ± 0.004 | 18,000 ± 2,000 |
A3X | 530 ± 13 | 0.059 ± 0.005 | 8,900 ± 700 | ND | ND | ND | 740 ± 14 | 0.052 ± 0.004 | 14,000 ± 1,000 |
Ad2+3XX | 540 ± 11 | 0.078 ± 0.004 | 6,900 ± 400 | ND | ND | ND | 810 ± 20 | 0.09 ± 0.01 | 9,000 ± 700 |
U4m2XX | 659 ± 9 | 0.30 ± 0.01 | 2,190 ± 60 | ND | ND | ND | ND | ND | ND |