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Table 1 Independent variables: coded and real value in center composite rotatable design

From: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Variables

Symbols

Levels

  

−1.68 (−α)

−1

0

+1

+1.68(+α)

Initial water added based on oil weight (wt.%)

A

0.30

2.00

4.50

7.00

8.70

Methanol to tung oil molar ratio (mol/mol)

B

0.98

2.00

3.50

5.00

6.02

Enzyme concentration based on oil weight (wt.%)

C

8.3

10.00

12.50

15.00

16.70