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Table 2 Relative abundance of Archaea in pretreated and un-pretreated sludge bioreactors at day 30 and 40

From: Towards a metagenomic understanding on enhanced biomethane production from waste activated sludge after pH 10 pretreatment

Archaeal taxonomic affiliation

Percentage of all Archaeal reads

(phylum/ family/ genus/ species)

UP30

UP40

P30

Euryarchaeota

97.60%

95.60%

94.86%

 Methanosarcinaceae

23.11%

20.56%

19.14%

  Methanosarcina

15.10%

13.32%

12.10%

 Methanobacteriaceae

20.81%

18.61%

21.44%

  Methanothermobacter

10.73%

9.41%

10.91%

  Methanothermobacter thermautotrophicus

9.50%

8.59%

9.94%

 Methanosaetaceae

14.00%

11.03%

9.19%

  Methanosaeta

13.13%

10.31%

8.56%

  Methanosaeta thermophila

14.48%

12.36%

10.25%

Crenarchaeota

2.33%

3.61%

4.40%

Korarchaeota

0.00%

0.45%

0.37%

Thaumarchaeota

0.05%

0.27%

0.37%

Nanoarchaeota

0.02%

0.07%

0.00%

  1. Legends: Archaeal domain consists three major families belonging to Methanosarcinaceae, Methanobacteriaceae and Methanosaetacea, while Methanosarcina, Methanosaeta and Methanothermobacter represent top abundant methanogens on the genus level. The decrease in abundance of Methanosarcina-related species in P30 was correlated with an enhanced bioproduction of acetate in the pretreated sludge. Except for phylum level, only candidates of abundance >8% are presented. Species are represented as percentage of all methanogens.