Figure 5
Raman spectra of native sugarcane bagasse (green online), pre-treated bagasse with oxalic acid (black online) and oxalic acid/enzymatic hydrolysis pre-treated bagasse (red line). A gradual reduction in band intensities was recorded in OAFEX and enzymatic hydrolysis revealing the displacement in hemicellulose backbone and enzymatic cleavage of β–1–4–glycosidic linkage in cellulose.