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Table 1 Effect of shikimate pathway intermediates chorismic acid (CA) and shikimic acid (SA) on the synthesis of 2-PE in P. putida CM12-5 (pPE-1) and CM12-5Δgcd (xylABE) (pPE-1) strains

From: Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida

C source

Supplement

[2-PE] (mg L−1)

CM12-5 (pPE-1)

CM12-5Δgcd (xylABE) (pPE-1)

M9 Glucose

None

39.3 ± 0.5

27.3 ± 0.1

+ 1 mM CA

104.3 ± 1.8

120.5 ± 0.9

+ 1 mM SA

72.9 ± 3.8

83.5 ± 2.2

M9 Xylose

None

16.0 ± 0.3

30.1 ± 0.4

+ 1 mM CA

105.4 ± 1.7

124.8 ± 1.6

+ 1 mM SA

51.4 ± 1.0

88.6 ± 3.7

  1. The assay was carried as described in the legend for Fig. 3 except that 1 mM chorismic acid (CA) or 1 mM shikimic acid (SA) were added. Values and standard deviation are the average of three independent assays