From: Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida
Strains/plasmids | Genotype/relevant features | References |
---|---|---|
Strains Escherichia coli | Â | Â |
DH5α | Cloning host: F-λ-endA1 glnX44(AS) thiE1 recA1 relA1 spoT1 gyrA96(NalR) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(rK–mK+) | [35] |
Pseudomonas putida | Â | Â |
DOT-T1E | Prototroph, CmR, RifR | [8] |
DOT-T1EΔgcd | DOT-T1E mutant generated by insertional inactivation of DOT-T1E_2882, KmR | [34] |
DOT-T1E (xylABE) | Wild type harboring plasmid pSEVA633_xylABE, GmR | [34] |
DOT-T1EΔgcd (xylABE) | DOT-T1E Δgcd mutant harboring plasmid pSEVA633_xylABE, KmR, GmR | [34] |
CM12-5 | DOT-T1E mutant that produces L-phenylalanine, RifR | [24] |
CM12-5Δgcd | CM12-5 mutant generated by insertional inactivation of DOT-T1E_2882, KmR | [34] |
CM12-5 (xylABE) | CM12-5 harboring plasmid pSEVA633_xylABE, GmR | [34] |
CM12-5Δgcd (xylABE) | CM12-5 Δgcd harboring plasmid pSEVA633_xylABE, GmR | [34] |
PG1E | CM12-5 derivative that grows in the presence of 10 mg mL−1 of FPA, RifR | This study |
PG2E | CM12-5 (pPHE1) derivative that grows in the presence of 10 mg mL−1 of FPA, RifR KmR | This study |
Plasmids | Â | Â |
pSEVA238 | Expression vector: oriV(pBBR1), xylS/ Pm, KmR | [67] |
pPHE1 | pSEVA238 derivative carrying pheAfbr gene from P. putida DOT-T1E | [24] |
pSEVA633 | Expression vector: oriV(pBBR1), lacZα-pUC18, GmR | [67] |
pSEVA633_xylABE | pSEVA633 with xylABE genes expressed from the EM7 promoter, GmR | [34] |
pSEVA438 | Expression vector: oriV(pBBR1), xylS/Pm, Sm/SpR | [67] |
pPE-1 | pSEVA438 derivative carrying PP_0968 from P. putida KT2440, kdc from Rhodospirillum rubrum, and T1E_5478 from P. putida DOT-T1E, SmR/SpR | [24] |
pSEVA433 | Expression vector: oriV(pBBR1), lacZα-pUC18, SmR/SpR | [67] |
pPE-2 | pSEVA433 derivative carrying PP_0968 from P. putida KT2440, kdc from Rhodospirillum rubrum, and T1E_5478 from P. putida DOT-T1E, SmR/SpR | This study |