Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste
© Almeida et al.; licensee BioMed Central Ltd. 2012
Received: 26 March 2012
Accepted: 29 June 2012
Published: 18 July 2012
The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a “waste-stream” instead of a valuable “coproduct”. The development of biorefineries, i.e. production of chemicals and power integrated with conversion processes of biomass into biofuels, has been singled out as a way to achieve economically viable production chains, valorize residues and coproducts, and reduce industrial waste disposal. In this sense, several alternatives aimed at the use of crude glycerol to produce fuels and chemicals by microbial fermentation have been evaluated. This review summarizes different strategies employed to produce biofuels and chemicals (1,3-propanediol, 2,3-butanediol, ethanol, n-butanol, organic acids, polyols and others) by microbial fermentation of glycerol. Initially, the industrial use of each chemical is briefly presented; then we systematically summarize and discuss the different strategies to produce each chemical, including selection and genetic engineering of producers, and optimization of process conditions to improve yield and productivity. Finally, the impact of the developments obtained until now are placed in perspective and opportunities and challenges for using crude glycerol to the development of biodiesel-based biorefineries are considered. In conclusion, the microbial fermentation of glycerol represents a remarkable alternative to add value to the biodiesel production chain helping the development of biorefineries, which will allow this biofuel to be more competitive.
KeywordsGlycerol Fermentation Biofuels Metabolic engineering Biodiesel
Production of biofuels and chemicals from renewable feedstocks is necessary to meet the energy demand in a world where petrol fuels are becoming scarce and more expensive. One of the main problems associated with biofuels is still the production costs, which can be reduced if residues of biofuels production processes are converted into valuable coproducts [1, 2]. Biodiesel is an alternative fuel that reduces net greenhouse effects and its use has become mandatory in many countries . It is mainly obtained by the transesterification of fat and vegetable oils in the presence of a catalyst by a primary alcohol (usually methanol) leading to a fatty acid methyl ester (FAME), which is used as a biofuel. Sunflower, rape, soybean and palm oils are the main substrates to make biodiesel worldwide, however, there are local variations on which is the main source. In Brazil, for example, 80% of the biodiesel produced in 2010 was from soybean oil .
In this review, we discuss the strategies to produce fuels and chemicals of biotechnological interest by microbial fermentation of glycerol. We highlight naturally occurring and engineered bacteria, yeast and filamentous fungi able to produce specific chemicals, as well as the strategies to improve their performance during fermentation. Market and industrial applications of these microbial fermentation products are also discussed.
Microbial fermentation of glycerol
The most studied route of biotechnological valorization of glycerol is related to its conversion to 1,3-propanediol (1,3-PDO). However, commercial production of 1,3-PDO from glycerol has not been reported. 1,3-PDO can be utilized for the synthesis of the modern polymer polytrimethylene terephthalate (PTT) that in turn can be used to make carpets (Corterra®, Shell), special textile fibers (Sorona®, DuPont), monofilaments, films, and nonwoven fabrics. PTT is also used in the engineering thermoplastics area . The demand of polybutylene terephthalate (PBT), another potential 1,3-PDO derivative, which is mainly consumed in the automotive and electronic sectors, in 2005 was estimated at about 376 000 tons with an average annual growth rate of 5 percent .
Chemicals produced at high yield and/or high concentration by microbial fermentation of glycerol
K. pneumoniae DSM 2026
K. pneumoniae LDH 526
C. butyricum F2b
E. coli K12
Fed-batch 1 m3
K. pneumoniae G31
K. pneumoniae G31
E. coli SY 4
G. oxydans ZJB09112
G. frateurii NBRC103465
A. tropicalis NBRC16470
E. coli AC-521
E. coli LA02Δdld
engineered E. coli
~ 4 g/g/h
Y. lipolytica Y-3314
0.45 g/g d
Y. lipolytica Wratislavia K1
D. hansenii SBP1
E. coli Arc2
Z. denitrificans MW1
2,3-Butanediol (BDO) can be employed in many chemical syntheses. For instance, it can be used to produce plastics, anti-freeze solutions and solvent preparations. In addition, it can be converted to methyl ethyl ketone (a liquid fuel additive), 1,3-butadiene (used to produce synthetic rubber), diacetyl (a flavoring agent), or to precursors of polyurethane (used in the pharmaceutical and cosmetics industries) [36, 37]. BDO is chemically obtained from petroleum; however, the development of a microbial production route based on renewable feedstocks is of interest. Fermentation of glycerol by several strains of Klebsiella spp. [11, 12, 18], resulted in BDO production. However, the main product of such fermentations is 1,3-PDO, whereas BDO is a minor product along with acetate, lactate, succinate, and ethanol.
Recently, a study demonstrated that BDO can be obtained as a major product of glycerol fermentation by K. pneumoniae G31 . Strain specificity and culture pH were the main factors influencing BDO production. Indeed, alternate production of BDO and acetic acid, the second most produced metabolite by K. pneumoniae G31 were demonstrated to be associated with pH self-control; i. e. during fermentation the microorganism alternates production of BDO and acetic acid to adjust pH of the cultures. In addition, BDO production was influenced by medium composition and aeration. When medium composition, aeration regime, and the initial pH of the fermentation were optimized, the production of BDO reached 70 g/L, the highest concentration obtained from glycerol, with a maximum yield of 0.39 g/g glycerol (Table 1) .
Ethanol is mainly used as fuel for transportation, but it can also be utilized in industry as a solvent and chemical intermediate. World fuel ethanol production, mainly based on yeast fermentation of sugarcane sucrose and corn starch, has reached 114 millions of cubic meters in 2011 . In addition, the development of technologies for ethanol production based on lignocellulosic feedstocks has been evaluated intensively [39, 40] and should also contribute to the increasing demand for this fuel. On the other hand, ethanol production from glycerol has gained modest attention in the last years.
Bacteria of the Enterobacteriaceae family and of the Clostridium genus are able to convert glycerol to ethanol, however, yields are relatively low because ethanol is only a secondary product of the fermentation [18, 19, 41]. In these cases, the main product of fermentation is 1,3-PDO [18, 41] or 2,3-butanediol . For instance, during pilot scale production of 1,3-PDO, concentrations of ethanol from glycerol varied from 8 to 17 g/L during fed-batch fermentation of K. pneumoniae[18, 41].
Recently it was demonstrated that E. coli can convert glycerol to ethanol anaerobically , as well as aerobically  (Figure 3). Initially, it was demonstrated that E. coli is able to ferment glycerol anaerobically in a pH-dependent manner, which is linked to CO2 availability. Glycerol fermentation proceeds under acidic conditions (pH6), however it is impaired under alkaline conditions (pH8) because CO2 availability is reduced (i.e., most CO2 is converted to bicarbonate) . Afterwards, several fold improvements in the production of ethanol and also of the coproducts hydrogen and formate was achieved by genetic engineering . Byproduct formation was reduced in the strains producing ethanol-hydrogen and ethanol-formate by mutations that inactivated fumarate reductase (DfrdA) and phosphate acetyltransferase (Dpta). To prevent the conversion of formate to CO2 and H2, the strain producing ethanol–formate also contained a mutation that inactivated formate–hydrogen lyase (DfdhF). Finally, high rates of glycerol utilization and product synthesis were obtained by simultaneous overexpression of glycerol dehydrogenase (gldA) and dihydroxyacetone kinase (dhaKLM). The production of ethanol from glycerol attained a yield of 85% of the theoretical maximum (the maximum theoretical yield is 1 mol each of H2 and ethanol per mol of glycerol fermented) in both strains . Fermentation performances of these strains were further improved by elimination of lactate production by deletion of ldhA, which encodes a lactate dehydrogenase, followed by fermentation under microaerobic conditions . Conversion rates of glycerol to ethanol as high as 85% of the theoretical maximum in mineral medium were obtained (Table 1). These results highlight the potential for using E. coli as a host for the production of ethanol from glycerol.
Production of ethanol from glycerol by the methylotrophic yeast Hansenula polymorpha was recently evaluated . A recombinant H. polymorpha yeast strain expressing the pdc and adhB genes, which encode, respectively, pyruvate decarboxylase and aldehyde dehydrogenase II, from Zymomonas mobilis exhibited a 3.3-fold increase in ethanol yield from glycerol fermentation under microaerobic conditions. However, the final concentration of ethanol obtained was still very low, i.e. 2.74 g/L .
Butanol has been identified as an alternative fuel and as a key chemical platform that can be industrially converted to acrylates, ethers, and butyl acetate, which in turn are utilized in paints, lacquers, and resin formulations . Butanol has been produced by Clostridium spp. fermentation of sugar for years. Clostridium pasteurianum can produce 1,3-PDO and butanol through anaerobic fermentation of glycerol . However, only recently the production of butanol from crude glycerol generated during biodiesel production was evaluated . C. pasteurianum was shown to produce butanol when grown in crude glycerol, although butanol yields and productivity on this substrate was considerably lower than on glycerol. Indeed, yields were approximately 20% higher on glycerol than in crude glycerol (from 0.36 g/g to 0.30 g/g (g butanol/glycerol consumed), whereas fermentation time was 2.5 times longer (i.e., 25 days instead of 10 days) on crude glycerol. The reduced yield and productivity on crude glycerol may be caused by contaminants in the crude glycerol, which contained approximately 90–95% glycerol, 5–10% methanol and/or water, and 3–5% sodium sulfate . These results indicate that C. pasteurianum may be used to produce butanol from biodiesel-derived crude glycerol to produce butanol, but its immediate industrial applicability is limited because of its slow growth and low butanol productivity.
Ketone and organic acids
Another important chemical produced from glycerol fermentation is dihydroxyacetone (DHA), the main active ingredient in all sunless tanning skincare products [47, 48]. DHA also serves as a versatile building block for the organic synthesis of a variety of fine chemicals. It is produced through oxidative fermentation by Gluconobacter oxydans via a membrane-bound glycerol dehydrogenase (GLY-DhdE) (Figure 3) [47, 48]. This appears to be the only reaction responsible for DHA synthesis and employs oxygen as the final acceptor of reduced equivalents, without NADH involvement. While DHA is produced by a GLY-DhdE, growth of G. oxydans on glycerol is ensured by a cytoplasmic pathway. In this pathway glycerol is phosphorylated to glycerol-3-phosphate (G3P) and then dehydrogenated to DHAP by glycerol kinase (GLY-Kin) and G3P-Dehydrogenase (G3P-Dhd), respectively. Finally, DHAP is catabolized in the pentose phosphate pathway (PPP) (Figure 3).
G. oxydans fermentation of glycerol for the production of dihydroxyacetone (DHA) is a process used in industry, however, there are problems related to microbial DHA production . For example, increased concentrations of glycerol inhibit DHA production and bacterial growth. In addition, production of glyceric acid (GA) by membrane-bound alcohol dehydrogenases (adhA) reduces production of DHA. To overcome these problems, a strain with disrupted AdhA with improved ability to grow in a higher concentration of glycerol (220 g/L) and to produce DHA compared to a wild-type strain (G. oxydans NBRC 12528) was generated. While the wild-type strain produced 38 g/L of DHA and 47 g/L GA when cultivated in 150 g/L of glycerol, the AdhA-disrupted strain produced only 2.2 g/L of GA and increased production of DHA to 108 g/L . Recently, an independent study demonstrated that optimization of growth medium and fermentation conditions to increase glycerol conversion to DHA by G. oxydans ZJB09112 lead to the production 161.9 g/L of DHA at a conversion rate of 88.7% in a fed-batch process (Table 1) .
While glyceric acid (GA) coproduction in the DHA production process is a problem, GA production by itself is of commercial interest. GA may be used in the chemical and pharmaceutical industries as a building block and for the production of polymers and surfactants . However, GA is not bulk produced, probably because a sizable number of applications for this chemical have not yet been developed. Because the GA molecule has three functional groups, it has a huge potential as a chemical that will add value to glycerol.
Like DHA, GA is mainly biotechnologically produced by bacteria, more specifically by the family of acetic acid bacteria (Acetobacteraceae), such as Gluconobacter sp., Acetobacter sp., and Gluconacetobacter sp. A membrane-bound Adh was shown to be involved in GA production by acetic acid bacteria (Figure 3) . Recently, the ability of 162 acetic acid bacterial strains to produce glyceric acid was evaluated regarding productivity and enantiomeric composition of the product . Productivity of glyceric acid varied from less than 10 g/L up to 40 g/L, whereas enantiomeric purity varied from less than 70% up to 99%. After optimization of glycerol concentration and aeration, two selected strains, Gluconobacter frateurii NBRC103465 and Acetobacter tropicalis NBRC16470, were able to produce more than 100 g/L of glyceric acid in fed-batch fermentation. G. frateurii NBRC103465 produced 136.5 g/L of glyceric acid with a 72% D-GA enantiomeric excess (ee), with yields of GA and DHA of 0.58 mol/mol and 0.12 mol/mol glycerol, respectively. On the other hand, A. tropicalis NBRC16470 produced 101.8 g/L of glyceric acid with 99% of enantiomeric excess (Table 1) . Identification of pathways responsible for the conversion of glycerol in these microorganisms is expected to allow the use of metabolic engineering strategies to reduce byproduct formation and lead to an industrial producer strain [24, 51].
Lactic acid has been used in the food industry for several years but has many other applications. Lactic acid can be processed to make acrylic acid or 1,2 propanediol used in polyester resins and polyurethane used as deicer or antifreeze . Lactate esters are used as green solvents for coating and in the cleaning industry, it can also be polymerized into the biobased polymer PLA (poly-lactic acid). As lactic acid became an important building block in the chemical industry, its microbial production has advanced significantly. Indeed, the availability of cheap and abundant residues from the biofuels industry is leading to a shift from chemical to microbial production of lactic acid.
Lactic acid bacteria fermentation processes in the food industry for production of lactic acid has a long history. Current processes for D-lactate production based on native lactic acid bacteria use sugars as carbon source and may also be applicable to lignocellulosic feedstocks [53, 54]. However, the utilization of lactic acid bacteria in industrial processes requires complex nutrients for cell growth and may not result in high product selectivity and enantiomeric purity .
Alternatively, lactic acid can also be produced from glycerol by other naturally producing microorganisms, including E. coli Klebsiella Clostridia Bacillus and the filamentous fungi Rhizopus oryzae[46, 56–59], although at very low concentrations and productivity. To overcome these issues, new strains have been screened and natural producers have been metabolically engineered to increase yield and production rate. In a screening of soil bacteria, the strain E. coli AC-521 was isolated based on its ability to use glycerol as carbon source and to grow quickly under aerobic conditions. This strain was able to produce lactic acid with a productivity of 0.49 g/g/h and a yield of 0.9 mol/mol glycerol (Table 1) .
Only recently E. coli has been engineered for homofermentative production of D-lactate from glycerol . Several enzymes leading to lactic acid production were overexpressed, while pathways leading to production of ethanol, succinate and acetate were blocked. The engineered E. coli strain was able to produce optically pure (99.9%) D-lactic acid from glycerol in minimal salts medium with only few supplements. In addition, this strain produced 32 g/L of D-lactate from 40 g/L of glycerol at a yield of 0.83 g/g glycerol, and with specific productivity for D-lactate production of 1.25 g/g cell mass/h .
Succinic acid is largely used for manufacturing health-related products, including pharmaceuticals, antibiotics, amino acids, and vitamins. In addition, it is an important building-block chemical that could be used to produce important precursors for chemical synthesis such as tetrahydrofuran, g-butyrolactone, 1,4-diaminobutane, 1,4-butanediol that are converted into a wide variety of products, including green solvents, pharmaceutical products, and biodegradable plastics . Natural succinate-producing rumen bacteria, such as Anaerobiospirillum succiniciproducens, can produce succinate from glycerol . But these bacterial strains require complex nutrients that increase production costs, purification, waste treatment and, consequently, hinder their utilization in an industrial process. Alternatively, the fermentative production of succinic acid from glycerol has been evaluated using either metabolically engineered Escherichia coli[27, 61, 62] or yeast strains (Table 1) [28, 63, 64].
Different strategies have been utilized to construct metabolically engineered E. coli strains for production of succinic acid from glycerol [27, 61, 62]. In a recent study, a strain was constructed in two steps and the use of a heterologous enzyme was needed . Initially, byproducts formation was avoided by blocking pathways leading to ethanol, acetic and lactic acid. Then, Lactococcus lactis pyruvate carboxylase (pyc) was expressed in this strain to drive the generation of succinate from the pyruvate node. The resulting strain was able to convert glycerol to succinic acid with a specific productivity of ~ 4 g/g/h and a yield of 0.69 g succinate/g glycerol, which is similar to yields obtained with glucose (0.78 g/g). However, fermentation under microaerobic conditions was necessary to overcome the need for rich nutrients and keep a net production of ATP. On the other hand, the use of microaerobic conditions may be a problem because it will lead to glycerol loss in form of CO2.
In a concurrent study, E. coli was engineered to produce succinate from glycerol using only native genes . Production of succinate was increased in 3 steps: i) coupling energy generation, i.e. ATP formation, to succinate production by increasing the activity of gluconeogenic phosphoenolpyruvate carboxykinase to perform the carboxylation of phosphoenolpyruvate; ii) avoiding formate and ethanol accumulation by inactivating pyruvate formate-lyase (pflB) and; iii) deleting ptsI (part of the intracellular phosphorelay system) which disrupts the primary pathway for anaerobic glycerol metabolism. With these modifications assembled, a strain able to produce succinate from glycerol with yields as high as 0.8 mol/mol glycerol was obtained. However, succinic acid productivity at anaerobic conditions was very low (i.e., 102 mM or 40 g/L of succinate was produced in 6 days).
Although the engineering strategies attempted so far resulted in strains with slow metabolism and growth on glycerol and/or need of oxygen supply during fermentation, they are important because they pave the way for the development of biotechnology applications using glycerol-derived succinate.
Alternatively, succinic acid can be produced by yeast. In a recent study a recombinant strain of the aerobic yeast, Yarrowia lipolytica, was able to produce succinic acid when cultivated on glycerol at low pH . The strain was constructed by deletion of the succinate dehydrogenase (SDH) subunit, which induces accumulation of succinic acid in Y. lipolytica, and also in fermentative yeasts as S. cerevisiae and Kluyveromyces lactis[28, 63, 64]. Unexpectedly, mutations in the succinate dehydrogenase subunit were shown to prevent Y. lipolytica growth on glucose, however, growth on glycerol and production of succinate was possible. Indeed, a strain with the SDH2 gene deleted was able to produce succinate from glycerol at the level of more than 45 g/L in shaking flasks with buffering and more than 17 g/L without buffering .
Citric acid is a weak organic acid that is commercially produced by fermentation of molasses (sucrose and glucose) by the fungus A. niger. As the citric acid global production has reached 1.6 million tons and keeps increasing annually at 3.5 - 4.0% in demand , its production from glycerol is also of interest. Among different potential producers of citric acid, the yeast Y. lipolytica has gained much attention in the last years as it is able to metabolize several important industrial and agro-industrial byproducts (i.e. saturated free fatty acids, raw glycerol) to produce large amounts of Single Cell Oil (SCO), organic acids and biosurfactant carbohydrate moieties (for instance, mannose and, rhamnose) . As expected, Y. lipolytica is able to synthesize citric acid using different carbon sources and secret it into the medium in conditions of excess carbon and nitrogen deficiency .
Several groups reported citric acid production from glycerol by Y. lipolytica but at low production levels and yields [6, 67, 68]. High production levels (i.e., above 100 g/L) were only obtained when better strains were selected [69, 70] and the cultivation mode was improved [5, 29, 71]. The importance of strain selection was highlighted when 27 strains of Y. lipolytica were compared based on the ability to produce citric acid from glycerol. Citric acid production in a nitrogen-limited medium varied from 1.4 g/l up to 21.6 g/L according to the strain utilized . Thus, identification of strains that produce not only lower levels of byproducts, such as isocitric acid and biomass, but also exhibit high yields and productivity will benefit the citric acid production process [69, 70].
During batch fermentation of glycerol to citric acid, the yeast is mainly affected by high concentrations of crude glycerol, its impurities, and the increasing concentrations of citric acid present at the end of fermentation. Indeed, productivity of citric acid was shown to decrease over time during crude glycerol fermentation by Y. lipolytica[5, 29]. To overcome these problems, at least partially, a repeated-batch strategy for the production of citric acid by Yarrowia using crude glycerol has been evaluated [5, 29]. The crude glycerol was composed by glycerol 76% (w/w), sodium salts 4% (w/w), methanol 0.1% w/w, metals Cu 0.3, Mg 100, Fe 13.7, Zn 2.9, and Ca 46 (ppm), other organic materials 0.8% (w/w) and water 19.5% (w/w). In this setup, cultivation was conducted in batch mode, then a portion of the culture liquid was withdrawn, and the same volume of the production medium was added. With that, cells were able to keep the production of citric acid as high as 124.2 g/L with a yield of 0.77 g/g and a productivity of 0.85 g/L/h for approximately 1000 h (Table 1) .
Oxalic acid is an organic acid known for its ability to leach iron oxides. It can be applied in industries, such in the manufacture of paper and detergents, to clean or bleach iron complexes . Production of oxalic acid by Aspergillus niger growing in crude glycerol waste from biodiesel production plants was recently demonstrated [30, 72]. Oxalic acid production reached approximately 21 g/L with a conversion yield of glycerol to oxalic acid (g/g) ranging from 0.55 to 0.62. These results look promising even considering the long production time of approximately 10 days. More recently, the ability of A. niger XP strain to produce oxalic acid was studied in submerged cultures containing 50 g/L of unpurified biodiesel-derived waste, which was composed of 45% (w/w) glycerol, 49% (w/w) free fatty acids, a low amount of fatty acid ethyl esters, and soaps from ethyl ester production . After 7 days of biosynthesis, the quantity of oxalic acid produced by A. niger XP reached 48.9 g/L with yields of oxalic acid as high as 0.88 (g. g − 1 glycerol consumed).
Polyols (also called sugar alcohols, polyhydric alcohols, or polyalcohols) are carbohydrates with a carbonyl group (aldehyde or ketone) reduced to a corresponding hydroxyl group. Examples of commercially available sugar alcohols include xylitol, sorbitol, mannitol, erythritol, lactitol, maltitol, and hydrogenated starch hydrolysate (HSH) . These compounds are used in a variety of applications, especially in the food, pharmaceutical, and medical industries, but they also serve as intermediates in chemical synthesis . Sugar alcohols share many attributes with sugars and have unique nutritional properties. They are used to improve the nutritional profile of food products due to their low caloric content, low insulin-mediated response, and non-cariogenicity [75–79]. These compounds and their derivatives also have other industrial applications, including the production of polyurethanes, plastifying agents, resins, surfactants, and intermediates for producing hydrocarbons [80–83].
In fact, sugar alcohols – especially sorbitol, xylitol, and arabitol – have been identified as belonging to a group of the 12 best chemical building blocks derived from biomass in studies by the U.S. Department of Energy . Sorbitol can be converted into chemicals such as propylene glycol, ethylene glycol, isosorbide, and anhydro sugars, whereas xylitol and arabitol can be converted into xylaric/xylonic acid, arabinoic/arabonic acid, and glycols [81, 84–86]. Due to their wide variety of applications, including their use in biorefineries, the production of polyols has increased worldwide. According to a recent study , the global market for polyols is expected to reach 1.81 million tons by the year 2015.
Market and current production processes of selected chemicals
Annual Production (t)
13,600 - 50,000
Chemical conversion of sugars
20,000 – 23,000
Due to the high cost of using certain pure sugars (for example, xylose, fructose, erythrose) for catalytic hydrogenation in comparison to the final product (sugar alcohols), many studies have instead described the use of cellulosic and hemicellulosic biomass hydrolysates as sources of carbon for fermentative polyol production . Although most sugar alcohols are industrially produced by chemical means, commercial production using microorganisms in fermentative processes is becoming a reality, as in the case for mannitol  and xylitol . With the advent of an abundant and cheap carbon source – such as crude glycerol from biodiesel production – new efforts have sought to either microbially produce sugar alcohols from this carbon source or employ it as a complementary source to the sugars traditionally used in the industry [31, 99]. Next the results from using glycerol or crude glycerol as sources of carbon for production of the polyols mannitol, erythritol, and arabitol with the aid of different microorganisms are discussed.
Some factors that influence the ability of two yeasts (Torulopsis mannitofaciens CBS 5981 and T. versatilis CBS 1752) to produce mannitol from glycerol have been investigated . In optimal conditions, T. mannitofaciens produced mannitol from glycerol consumed with a yield of 31% of the theorethycal maximum. In addition, it was shown that high concentrations of nitrogen sources and KH2PO4 in the culture medium significantly reduced mannitol yield despite consumption of glycerol. Previous studies reported Candida magnoliae as excellent mannitol producer using glucose and fructose mixtures as carbon sources . A more recent study investigated mannitol production from glycerol using resting cells of this species . It was shown that C. magnoliae was able to consume 100 g/L glycerol in 96 h, resulting in 51 g/L mannitol, which corresponds to a yield of 0.51 g/g. In addition, mannitol was the only metabolite detectable by UV–VIS or RI detector when samples from resting cells of C. magnolia were analyzed using ion exclusion high performance liquid chromatography. The absence of other metabolites in solution may facilitate mannitol recovery. Khan et al. (2009) also found that the mannitol yield was negatively affected when glycerol was supplemented with KH2PO4 and yeast extract.
André and co-workers  investigated the ability of different strains of Yarrowia lipolytica to convert residual crude glycerol (glycerol 70% w/w, impurities composed of potassium and sodium salts (12% w/w), non-glycerol organic material (1% w/w), methanol (2% w/w) and water (14% w/w)) from biodiesel production into chemical compounds with higher aggregate value. In this study, the strains Y. lipolytica LFMB 19, Y. lipolytica LFMB 20, and Y. lipolytica ACA-YC 5033 were cultured in nitrogen-limited culture medium with a residual crude glycerol concentration of 30 g/L. Under these conditions, the main metabolic product synthesized by the strains LFMB 19 and LFMB 20 was mannitol (6.0 g/L maximum quantity and yield of 0.2 to 0.26 g per g of crude glycerol consumed). This metabolite was produced in negligible quantity by the ACA-YC 5033 strain . In an independent study , other 15 strains of different species of yeast and filamentous fungi of the class Zygomycetes were compared on their ability to convert crude glycerol into different chemical compounds. Increasing concentrations of crude glycerol (30, 60, and 90 g/L) were used to screen the strains under nitrogen-limited conditions. Y. lipolytica Pichia membranifaciens, and Thamnidium elegans were able to grow in culture medium containing high initial glycerol concentrations without inhibition by the substrate. In the case of Y. lipolytica, the best producer of mannitol under the conditions analyzed, there was a positive correlation between the increase in crude glycerol concentration in the initial culture medium and mannitol production, with mannitol concentration and with yields reaching 19.4 g/L and 0.23 g/g, respectively .
These examples demonstrate the importance of evaluating different microbial strains for mannitol production from crude glycerol to select the most promising strains. Although the production of mannitol on commercial scale by bacteria using conventional carbon sources has been accomplished , reports on the bioconversion of crude glycerol into mannitol by bacteria are scarce. Furthermore, the previous examples reveal the need for further studies on the biochemical events that lead to biosynthesis of mannitol and other polyols; for example, Y. lipolytica strains grown in residual crude glycerol.
Commercial erythritol production occurs exclusively via fermentation in substrates containing sugars, such as glucose and fructose, from the hydrolysis of biomass. Although fermentative erythritol production by different microorganisms has been studied since 1960 [79, 104], few studies have investigated the production of this polyol from different carbon sources . In the specific case of using residual crude glycerol, containing 550 g/L of glycerol and 50 g/L of KCl, as a carbon source, an acetate-negative mutant of Y. lipolytica (Wratislavia K1) was found to simultaneously produce significant quantities of erythritol and citric acid . With an initial crude glycerol concentration of 150 g/L in nitrogen-limited culture medium and a pH of 5.5 favorable for producing citric acid, a concentration of 81 g/L erythritol was obtained after fed-batch fermentation for 97 h.
Subsequently, the effect of pH on erythritol production by the Wratislavia K1 mutant of Y. lipolytica was investigated . Erythritol concentration reached 170 g/L (0.56 g/g yield) after fed-batch growth with a total concentration of 300 g/L crude glycerol and pH of 3.0. Under these conditions, citric acid was not produced, demonstrating that this pH range is optimal for the mutant to produce erythritol, as it prevents the channeling of glycerol to citric acid production . This example demonstrated that by using residual crude glycerol as the carbon source, the yields of erythritol without the generation of undesirable byproducts were comparable to the reported yields with microorganisms used in commercial erythritol production with glucose as substrate . As previously reported for mannitol production, it is noteworthy that approaches involving bioprospecting microorganisms able to tolerate high osmolarity  can lead to a higher yield relative to erythritol-producing industrial strains.
Arabitol polyol has many attributes of its enantiomer, xylitol, making its use feasible in many known applications of xylitol, such as in natural sweeteners, caries reducers, and sugar substitutes for diabetic patients . Arabitol and xylitol can be transformed into arabinoic/arabonic and xylaric/xylonic acids, which in turn can be used to produce unsaturated polyesters and polymers with new applications [84, 86]. This role indicates their importance in the biorefinery context. In addition, arabitol can be biologically converted into xylitol, for example, by Gluconobacter oxidans[107, 108], representing a possible efficient route for the synthesis of xylitol of a higher purity and specificity.
In this scenario, Koganti  conducted an extensive screening of over 214 yeast strains belonging to 25 different genera from the Agricultural Research Service Culture Collection (NRRL) with regard to their ability to produce arabitol/xylitol using crude glycerol from biodiesel production. In this study, xylitol yield was low for most strains; however, the Debaryomyces hansenii SBP-1 strain was selected for its ability to produce high arabitol concentrations as the only polyol, thus facilitating further separation of the product. Arabitol production by D. hansenii SBP-1 was then investigated under different conditions, and a yield of 0.5 g/g glycerol consumed was attained with 150 g/L crude glycerol in the initial culture medium under aerobic conditions at 30°C.
Subsequently, culture conditions for the D. hansenii SBP-1 strain were optimized , leading to an arabitol yield of 0.6 g/g and volumetric productivity of 0.35 g/L/h. A protocol was developed for separating arabitol from the fermentation medium containing 20–30 g/L glycerol and 40–50 g/L arabitol, which led to a 60% arabitol yield with 95% purity.
Polyhydroxyalkanoates (PHAs) have received great attention due to their potential application as renewable, biodegradable, and biocompatible thermoplastics . They can be completely degraded by microorganisms present in most environments, and can be produced from different renewable carbon sources . Poly-3-hydroxybutyrate PHB belongs to the group of polyhydroxyalkanoates (PHAs) and it is the best-studied example of biodegradable polyesters. This polymer is naturally synthesized by a wide variety of bacterial species as a storage compound for carbon and energy . Nowadays, commercial production of PHB is small and limited by substrate cost . Thus, efficient conversion of cheap crude glycerol from the biodiesel industry to PHB is an interesting opportunity to reduce production costs and make PHB an industrial biotechnological product [34, 35]. Indeed, microbial PHB synthesis from glycerol has been evaluated with several PHA producers and under different fermentation conditions [34, 35, 113–116].
Conversion of glycerol to PHBs has reached high production levels due to optimization of strains and fermentations conditions. Implementation of fed-batch under low O2 conditions appeared to be a suitable strategy for the production of PHB by the genetic engineered E. coli Arc2 strain . In microaerobic fed-batch cultures in which glycerol was added to maintain its concentration above 5 g/L, cell dry weight and PHB concentration reached 21.17 and 10.81 g/L, respectively, after 60 h (Table 1) . Similarly, fed-batch cultivation improved PHB production by the newly isolated bacterium, Zobellella denitrificans MW1, which was characterized as producing large amounts of PHB from glycerol in presence of NaCl [35, 114]. Cultivation in the medium containing 20 g/L NaCl, with optimized feeding of glycerol and ammonia, resulted in a PHB content of 66.9% of cell dry weight, and the polymer productivity and substrate yield coefficient of 1.09 g/L/h and 0.25 g PHB/g glycerol, respectively .
Conversion of glycerol to D-xylulose has been achieved using a two stage microbial fermentation. Initially, glycerol was converted to D-arabitol by Candida parapsilosis, then yeast cells were collected and finally the D-arabitol from the culture supernatant was converted to D-xylulose by G. oxydans. However, this conversion process needs to be improved before increasing production scale since from 170 g/L glycerol only 32.2 g/L of D-arabitol was produced.
Polyunsaturated fatty acids
Polyunsaturated fatty acids, such has docosahexaenoic acid (DHA, 22:6), eicosapentanoic acid (EPA, 20:5) and α-linoleic acid (ALA, 18:3) have market presence as nutraceuticals, because they are beneficial for human health. The use of biodiesel-derived glycerol as carbon source to produce such polyunsatured fatty acids, especially DHA, was recently reviewed [118, 119]. Thus we do not further discuss this route for valorization of crude glycerol in this work.
Biohydrogen and biomethane
Crude glycerol utilization to produce gaseous fuels, biohydrogen and biomethane (biogas), has gained more attention lately (as reviewed by [119, 120]. Indeed, several studies have reported biohydrogen and biomethane production by microbial conversion processes from crude glycerol using single microbial strains or bacterial communities. Utilization of crude glycerol as the sole carbon source or as a suitable co-substrate to improve the efficiency of biodigesters has also been investigated. These topics were currently reviewed and reported in recent publications [119–122] and will not be further discussed here.
Biodiesel-based biorefinery: opportunities and challenges
Biorefineries are based on the integration of the biomass conversion processes to produce power, fuels and chemicals. In this context, the utilization of glycerol generated in the biodiesel production process offers an excellent opportunity to obtain chemicals by microbial fermentation. Production yields of fuels and chemicals from glycerol as high as 90% of theoretical maximum have been obtained (Table 1). These high production yields of the desired product should make the establishment of bioprocesses easier. Even if the available producing strains have not been evaluated at pilot scale yet (at least based on the publicly available data), market demand for green chemicals, new processes, and technologies for lignocelulose biorefineries should facilitate the development of industrial processes based on crude glycerol.
A vast range of fuels and chemicals can potentially be produced by microbial fermentation of glycerol. However, the expectation is that the primary products obtained by fermentation in biorefineries are chemicals with established demands and international markets. In fact, from the fourteen compounds produced at lab scale by microbial fermentation of glycerol showed in Table 1, ten of them are nowadays produced by chemical or microbial conversion of sugars or petrochemical processes. More interesting, these chemicals are important platform chemicals or products that have a consolidated market demand of thousands of tons per year (Table 2). Thus, once a production process based on glycerol fermentation for any of these chemicals is developed, the product could easily enter the market. On the other hand, it is important that production costs of chemicals from glycerol remain competitive with those obtained from petroleum. It should be noted that demands for sustainable development and volatile petroleum prices should favor the use of “greener” chemicals in the long term, even if their market price is not considerably cheaper. Chemicals without or with a limited market, like DHA and glyceric acid, may represent an opportunity for the development of new products as there is no direct benchmark production price they will need to compete against.
Manipulation of microbes to allow the cost competitive commercial production of fuels and chemicals, such as ethanol, butanol, isoprenoids and others, on sugar and lignocellulose has advanced significantly in the last years . Indeed, several processes based on engineered microorganisms, especially yeast and bacteria species, have been developed and implemented . Two examples of success are given by the production of 1,3-PDO and succinic acid. The chemical 1,3-PDO is traditionally made from fossil-derived ethylene oxide or propylene, however, a bio-based process has been developed and implemented by DuPont and Tate & Lyle. Their process for production of 1,3-PDO relies on a microbe expressing genes from several different microorganisms to give the required productivity . The process based on the use of a designer microbe to produce 1,3-PDO from corn has been running in a Bio-PDO™ plant in Tennessee (USA) with a capacity of 45 thousand tons a year since 2007. Similar approaches are being developed for succinic acid. Bio-based succinic acid production by a fermentative technology is the focus of a joint venture between DSM and Roquette. Indeed, the first testing volumes of this renewable and versatile chemical produced from corn have already been produced in a demonstration plant in Lestrem (France) that was built in 2009. These developments are expected to facilitate the establishment of glycerol-based fermentation processes, since similar microorganisms and pathways may be used.
Although the challenges of obtaining microbial strains able to operate under industrial process conditions have been overcome, another challenge for the production of fuels and chemicals from biodiesel industry crude glycerol is the supply chain. From the economic point of view, it is important for the industry that the feedstocks for biodiesel production, and consequently glycerol for fuels and chemicals production, are abundant year round. In this sense, some countries may not have sufficient biomass, and consequently glycerol, to maintain an industrial-scale production of biodiesel, fuels and chemicals throughout the four seasons, especially due to competition of biomass for other uses. One way to solve this problem, especially in countries with vast territory and mild climate year round, is the diversification of biomass feedstocks in the industry and regionalization of production plants. In this sense, the main feedstock for the industry should be growing in the region of the production plant, which may only be complemented by alternative biomass from other regions. In countries like Brazil, for example, where 80% of biodiesel is produced from soybean oil, it might be advantageous to start using oil from alternative biomass sources, like palm oil, physic nut, castor bean and others. This would guarantee the supply of biomass for the industry and at the same time avoid transportation of soybean to production plants around the country.
Increasing awareness of environmental issues and consequent pressure from governments and public agencies to reduce the emission of pollutants, together with the increasing petroleum cost and demand for fuels and chemicals worldwide have led to the development of biomass conversion processes. Processes for production of fuels and chemicals from crude glycerol waste from the biodiesel industry have been evaluated and developed at laboratory scale. Indeed, the strong potential of crude glycerol use for the development of biorefineries has been demonstrated by the production of several chemicals using different routes and microorganisms. Several yeasts and bacteria, especially E. coli and others from the Enterobacteriaceae and Clostridiaceae families, have been evaluated for fermentation of glycerol. Efficient selection and construction of recombinant strains based on biochemical and genomic data associated with optimization of fermentation conditions resulted in strains able to produce more than 100 g/L of the desired products with yields above 50% of the theorethycal maximum in laboratory scale.
Among the different routes to establish a fermentative process based on microbial fermentation of glycerol, the use of “wild type” natural producers and engineered strains are the most considered. The later case is more common for bacteria of the Enterobacteriaceae family and the former one for engineered strains of yeast and E. coli. When wild type strains are employed to produce the desired chemical, efforts to improve production generally concentrate on the optimization of fermentation conditions, like aeration, pH, substrate concentration and feeding. However, the use of these strains (species) in industrial applications may be impaired by pathogenicity, need for strict anaerobic conditions, or lack of genetic tools. The use of engineered strains commonly employed in industry, especially yeasts and E. coli, can solve this problem. However, metabolic engineering strategies have to be used to drive metabolite synthesis through homologous and heterologous pathways. Although in industry the most commonly used strains are engineered producers of fuels and chemicals, a careful screening of the biodiversity is generally advisable, since microorganisms can naturally produce a vast range of compounds. Once enzymes and metabolic pathways in such microorganisms are identified and isolated they can be used for the development of recombinant strains.
Considering the already remarkable advances for the production of fuels and chemicals from glycerol using microorganisms, it may be expected that strains for industrial implementation of these bioprocesses should become available in the next years. The technology for the production of butanol, propanodiol and succinic acid from simple renewable feedstocks such as starch and sucrose is already available. Therefore, it is expected that these chemicals will be the first to be produced from more complex substrates such as biodiesel-derived crude glycerol and other more complex biomasses. However, before implementation of a pilot or industrial plant to produce any fuel or chemical, two important aspects should be considered: i) the cost of producing fuels and chemicals by microbial fermentation should be comparable with fossil-derived products; and ii) the feedstock supply chain for the industry.
This work was financially supported by the Brazilian National Council for Scientific and Technological Development (CNPq), FINEP and Embrapa.
- Zhang Y-HP: What is vital (and not vital) to advance economically-competitive biofuels production. Process Biochem. 2011, 46 (11): 2091-2110. 10.1016/j.procbio.2011.08.005.Google Scholar
- Yazdani SS, Gonzalez R: Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotech. 2007, 18: 213-219. 10.1016/j.copbio.2007.05.002.Google Scholar
- O’Connor D: Report T39-T3. Biodiesel GHG emissions, pas, present, and future . A report to IEA Bioenergy Task 39. Commercializing Liquid Biofuels from Biomass. 2011, International Energy Agency (IEA), , www.ieabioenergy.com/Liblinks.aspx,Google Scholar
- ANP: Agência Nacional do Petróleo, Gás natural e Biocombustíveis. Boletim mensal de biodiesel. 2011, Junho: http://www.anp.gov.br/?pg=59236&m=&t59231=&t59232=&t59233=&t59234=&ar=&ps=&cachebust=1342442475715,Google Scholar
- Rywinska A, Rymowicz W: High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors. J Ind Microbiol Biot. 2010, 37: 431-435. 10.1007/s10295-009-0687-8.Google Scholar
- Papanikolaou S, Fakas S, Fick M, Chevalot I, Galiotou-Panayotou M, Komaitis M, Marc I, Aggelis G: Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil. Biomass Bioenerg. 2008, 32: 60-71. 10.1016/j.biombioe.2007.06.007.Google Scholar
- Koutinas AA, Wang RH, Webb C: The biochemurgist - Bioconversion of agricultural raw materials for chemical production. Biofuel Bioprod Bior. 2007, 1: 24-38. 10.1002/bbb.6.Google Scholar
- Liu HJ, Xu YZ, Zheng ZM, Liu DH: 1,3-Propanediol and its copolymers: Research, development and industrialization. Biotechnol J. 2010, 5: 1137-1148. 10.1002/biot.201000140.Google Scholar
- NNDCC: Biochemical Opportunities in the United Kingdom,. 2008, www.nnfcc.co.uk/publications,Google Scholar
- Celinska E: Debottlenecking the 1,3-propanediol pathway by metabolic engineering. Biotechnol Adv. 2010, 28: 519-530. 10.1016/j.biotechadv.2010.03.003.Google Scholar
- Biebl H, Menzel K, Zeng AP, Deckwer WD: Microbial production of 1,3-propanediol. Appl Microbiol Biotechnol. 1999, 52: 289-297. 10.1007/s002530051523.Google Scholar
- Mu Y, Teng H, Zhang DJ, Wang W, Xiu ZL: Microbial production of 1,3-propanediol by Klebsiella pneumoniae using crude glycerol from biodiesel preparations. Biotechnol Lett. 2006, 28: 1755-1759. 10.1007/s10529-006-9154-z.Google Scholar
- Chen X, Zhang DJ, Qi WT, Gao SJ, Xiu ZL, Xu P: Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions. Appl Microbiol Biotechnol. 2003, 63: 143-146. 10.1007/s00253-003-1369-5.Google Scholar
- Lin RH, Liu HJ, Hao J, Cheng K, Liu DH: Enhancement of 1,3-propanediol production by Klebsiella pneumoniae with fumarate addition. Biotechnol Lett. 2005, 27: 1755-1759. 10.1007/s10529-005-3549-0.Google Scholar
- Hong WK, Kim CH, Heo SY, Luo LH, Oh BR, Rairakhwada D, Seo JW: 1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae. Bioprocess Biosyst Eng. 2011, 34: 231-236. 10.1007/s00449-010-0465-z.Google Scholar
- Tang XM, Tan YS, Zhu H, Zhao K, Shen W: Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli. Appl Environ Microb. 2009, 75: 1628-1634. 10.1128/AEM.02376-08.Google Scholar
- Xu YZ, Guo NN, Zheng ZM, Ou XJ, Liu HJ, Liu DH: Metabolism in 1,3-propanediol fed-batch fermentation by a D-lactate deficient mutant of Klebsiella pneumoniae. Biotechnol Bioeng. 2009, 104: 965-972. 10.1002/bit.22455.Google Scholar
- Liu HJ, Zhang DJ, Xu YH, Mu Y, Sun YQ, Xiu ZL: Microbial production of 1,3-propanediol from glycerol by Klebsiella pneumoniae under micro-aerobic conditions up to a pilot scale. Biotechnol Lett. 2007, 29: 1281-1285. 10.1007/s10529-007-9398-2.Google Scholar
- Petrov K, Petrova P: High production of 2,3-butanediol from glycerol by Klebsiella pneumoniae G31. Appl Microbiol Biotechnol. 2009, 84: 659-665. 10.1007/s00253-009-2004-x.Google Scholar
- Petrov K, Petrova P: Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations. Appl Microbiol Biotechnol. 2010, 87: 943-949. 10.1007/s00253-010-2545-z.Google Scholar
- Durnin G, Clomburg J, Yeates Z, Alvarez PJJ, Zygourakis K, Campbell P, Gonzalez R: Understanding and Harnessing the Microaerobic Metabolism of Glycerol in Escherichia coli. Biotechnol Bioeng. 2009, 103: 148-161. 10.1002/bit.22246.Google Scholar
- Taconi KA, Venkataramanan KP, Johnson DT: Growth and Solvent Production by Clostridium pasteurianum ATCC (R) 6013 (TM) Utilizing Biodiesel-Derived Crude Glycerol as the Sole Carbon Source. Environ Prog Sustain Energy. 2009, 28: 100-110. 10.1002/ep.10350.Google Scholar
- Hu ZC, Liu ZQ, Zheng YG, Shen YC: Production of 1,3-Dihydroxyacetone from Glycerol by Gluconobacter oxydans ZJB09112. J Microbiol Biotechn. 2010, 20: 340-345.Google Scholar
- Habe H, Shimada Y, Yakushi T, Hattori H, Ano Y, Fukuoka T, Kitamoto D, Itagaki M, Watanabe K, Yanagishita H, et al: Microbial Production of Glyceric Acid, an Organic Acid That Can Be Mass Produced from Glycerol. Appl Environ Microb. 2009, 75: 7760-7766. 10.1128/AEM.01535-09.Google Scholar
- Hong AA, Cheng KK, Peng F, Zhou S, Sun Y, Liu CM, Liu DH: Strain isolation and optimization of process parameters for bioconversion of glycerol to lactic acid. J Chem Technol Biot. 2009, 84: 1576-1581. 10.1002/jctb.2209.Google Scholar
- Mazumdar S, Clomburg JM, Gonzalez R: Escherichia coli strains engineered for homofermentative production of D-lactic acid from glycerol. Appl Environ Microb. 2010, 76: 4327-4336. 10.1128/AEM.00664-10.Google Scholar
- Blankschien MD, Clomburg JM, Gonzalez R: Metabolic engineering of Escherichia coli for the production of succinate from glycerol. Metab Eng. 2010, 12: 409-419. 10.1016/j.ymben.2010.06.002.Google Scholar
- Yuzbashev TV, Yuzbasheva EY, Sobolevskaya TI, Laptev IA, Vybornaya TV, Larina AS, Matsui K, Fukui K, Sineoky SP: Production of Succinic Acid at Low pH by a Recombinant Strain of the Aerobic Yeast Yarrowia lipolytica. Biotechnol Bioeng. 2010, 107: 673-682. 10.1002/bit.22859.Google Scholar
- Rymowicz W, Fatykhova AR, Kamzolova SV, Rywinska A, Morgunov IG: Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes. Appl Microbiol Biotechnol. 2010, 87: 971-979. 10.1007/s00253-010-2561-z.Google Scholar
- Andre A, Diamantopoulou P, Philippoussis A, Sarris D, Komaitis M, Papanikolaou S: Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid. Ind Crop Prod. 2010, 31: 407-416. 10.1016/j.indcrop.2009.12.011.Google Scholar
- Khan A, Bhide A, Gadre R: Mannitol production from glycerol by resting cells of Candida magnoliae. Bioresour Technol. 2009, 100: 4911-4913. 10.1016/j.biortech.2009.04.048.Google Scholar
- Rymowicz W, Rywinska A, Marcinkiewicz M: High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica. Biotechnol Lett. 2009, 31: 377-380. 10.1007/s10529-008-9884-1.Google Scholar
- Koganti S, Kuo TM, Kurtzman CP, Smith N, Ju LK: Production of arabitol from glycerol: strain screening and study of factors affecting production yield. Appl Microbiol Biotechnol. 2011, 90: 257-267. 10.1007/s00253-010-3015-3.Google Scholar
- Nikel PI, Pettinari MJ, Galvagno MA, Mendez BS: Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures. Appl Microbiol Biotechnol. 2008, 77: 1337-1343. 10.1007/s00253-007-1255-7.Google Scholar
- Ibrahim MHA, Steinbuchel A: Poly(3-Hydroxybutyrate) Production from Glycerol by Zobellella denitrificans MW1 via High-Cell-Density Fed-Batch Fermentation and Simplified Solvent Extraction. Appl Environ Microb. 2009, 75: 6222-6231. 10.1128/AEM.01162-09.Google Scholar
- Syu MJ: Biological production of 2,3-butanediol. Appl Microbiol Biotechnol. 2001, 55: 10-18. 10.1007/s002530000486.Google Scholar
- Ji XJ, Huang H, Ouyang PK: Microbial 2,3-butanediol production: A state-of-the-art review. Biotechnol Adv. 2011, 29: 351-364. 10.1016/j.biotechadv.2011.01.007.Google Scholar
- Renewable Fuels Association, Industry Statistics, Ethanol Industry, Overview: World Fuel Ethanol Production,. http://ethanolrfa.org/pages/World-Fuel-Ethanol-Production,
- Almeida JRM, Runquist D, Nogue VSI, Liden G, Gorwa-Grauslund MF: Stress-related challenges in pentose fermentation to ethanol by the yeast Saccharomyces cerevisiae. Biotechnol J. 2011, 6: 286-299. 10.1002/biot.201000301.Google Scholar
- Geddes CC, Nieves IU, Ingram LO: Advances in ethanol production. Curr Opin Biotech. 2011, 22: 312-319. 10.1016/j.copbio.2011.04.012.Google Scholar
- Cheng KK, Zhang JA, Liu DH, Sun Y, Liu HJ, Yang MD, Xu JM: Pilot-scale production of 1,3-propanediol using Klebsiella pneumoniae. Process Biochem. 2007, 42: 740-744. 10.1016/j.procbio.2007.01.001.Google Scholar
- Dharmadi Y, Murarka A, Gonzalez R: Anaerobic fermentation of glycerol by Escherichia coli: A new platform for metabolic engineering. Biotechnol Bioeng. 2006, 94: 821-829. 10.1002/bit.21025.Google Scholar
- Yazdani SS, Gonzalez R: Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab Eng. 2008, 10: 340-351. 10.1016/j.ymben.2008.08.005.Google Scholar
- Hong WK, Kim CH, Heo SY, Luo L, Oh BR, Seo JW: Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis. Biotechnol Lett. 2010, 32: 1077-1082. 10.1007/s10529-010-0259-z.Google Scholar
- Harvey BG, Meylemans HA: The role of butanol in the development of sustainable fuel technologies. J Chem Technol Biot. 2011, 86: 2-9. 10.1002/jctb.2540.Google Scholar
- Biebl H: Fermentation of glycerol by Clostridium pasteurianum - batch and continuous culture studies. J Ind Microbiol Biot. 2001, 27: 18-26. 10.1038/sj.jim.7000155.Google Scholar
- Hekmat D, Bauer R, Fricke J: Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng. 2003, 26: 109-116. 10.1007/s00449-003-0338-9.Google Scholar
- Bauer R, Katsikis N, Varga S, Hekmat D: Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process. Bioprocess Biosyst Eng. 2005, 28: 37-43. 10.1007/s00449-005-0009-0.Google Scholar
- Habe H, Fukuoka T, Morita T, Kitamoto D, Yakushi T, Matsushita K, Sakaki K: Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans. Biosci Biotech Bioch. 2010, 74: 1391-1395. 10.1271/bbb.100068.Google Scholar
- Habe H, Fukuoka T, Kitamoto D, Sakaki K: Biotechnological production of D-glyceric acid and its application. Appl Microbiol Biotechnol. 2009, 84: 445-452. 10.1007/s00253-009-2124-3.Google Scholar
- Habe H, Shimada Y, Fukuoka T, Kitamoto D, Itagaki M, Watanabe K, Yanagishita H, Yakushi T, Matsushita K, Sakaki K: Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol. Biosci Biotech Bioch. 2010, 74: 2330-2332. 10.1271/bbb.100406.Google Scholar
- Sodergard A, Stolt M: Properties of lactic acid based polymers and their correlation with composition. Prog Polym Sci. 2002, 27: 1123-1163. 10.1016/S0079-6700(02)00012-6.Google Scholar
- Dien BS, Nichols NN, Bothast RJ: Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars. J Ind Microbiol Biot. 2001, 27: 259-264. 10.1038/sj.jim.7000195.Google Scholar
- Sauer M, Porro D, Mattanovich D, Branduardi P: Microbial production of organic acids: expanding the markets. Trends Biotechnol. 2008, 26: 100-108. 10.1016/j.tibtech.2007.11.006.Google Scholar
- Hofvendahl K, Hahn-Hagerdal B: Factors affecting the fermentative lactic acid production from renewable resources. Enzyme Microb Tech. 2000, 26: 87-107. 10.1016/S0141-0229(99)00155-6.Google Scholar
- Cheng KK, Zhang JA, Liu DH, Sun Y, Yang MD, Xu JM: Production of 1,3-propanediol by Klebsiella pneumoniae from glycerol broth. Biotechnol Lett. 2006, 28: 1817-1821. 10.1007/s10529-006-9158-8.Google Scholar
- El-Ziney MG, Arneborg N, Uyttendaele M, Debevere J, Jakobsen M: Characterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures. Biotechnol Lett. 1998, 20: 913-916. 10.1023/A:1005434316757.Google Scholar
- Payot T, Chemaly Z, Fick M: Lactic acid production by Bacillus coagulans - Kinetic studies and optimization of culture medium for batch and continuous fermentations. Enzyme Microb Tech. 1999, 24: 191-199. 10.1016/S0141-0229(98)00098-2.Google Scholar
- Bai DM, Jia MZ, Zhao XM, Ban R, Shen F, Li XG, Xu SM: L(+)-lactic acid production by pellet-form Rhizopus oryzae R1021 in a stirred tank fermentor. Chem Eng Sci. 2003, 58: 785-791. 10.1016/S0009-2509(02)00608-5.Google Scholar
- Lee PC, Lee WG, Lee SY, Chang HN: Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnol Bioeng. 2001, 72: 41-48. 10.1002/1097-0290(20010105)72:1<41::AID-BIT6>3.0.CO;2-N.Google Scholar
- Zhang X, Shanmugam KT, Ingram LO: Fermentation of glycerol to succinate by metabolically engineered strains of Escherichia coli. Appl Environ Microb. 2010, 76: 2397-2401. 10.1128/AEM.02902-09.Google Scholar
- Zhang X, Jantama K, Moore JC, Jarboe LR, Shanmugam KT, Ingram LO: Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proc Natl Acad Sci U S A. 2009, 106: 20180-20185. 10.1073/pnas.0905396106.Google Scholar
- Arikawa Y, Kuroyanagi T, Shimosaka M, Muratsubaki H, Enomoto K, Kodaira R, Okazaki M: Effect of gene disruptions of the TCA cycle on production of succinic acid in Saccharomyces cerevisiae. J Biosci Bioeng. 1999, 87: 28-36. 10.1016/S1389-1723(99)80004-8.Google Scholar
- Saliola M, Bartoccioni PC, De Maria I, Lodi T, Falcone C: The deletion of the succinate dehydrogenase gene KlSDH1 in Kluyveromyces lactis does not lead to respiratory deficiency. Eukaryot Cell. 2004, 3: 589-597. 10.1128/EC.3.3.589-597.2004.Google Scholar
- Anastassiadis S, Morgunov IG, Kamzolova SV, Finogenova TV: Citric acid production patent review. Recent Pat Biotechnol. 2008, 2: 107-123. 10.2174/187220808784619757.Google Scholar
- Amaral PFF, Ferreira TF, Fontes GC, Coelho MAZ: Glycerol valorization: New biotechnological routes. Food Bioprod Process. 2009, 87: 179-186. 10.1016/j.fbp.2009.03.008.Google Scholar
- Papanikolaou S, Muniglia L, Chevalot I, Aggelis G, Marc I: Yarrowia lipolytica as a potential producer of citric acid from raw glycerol. J Appl Microbiol. 2002, 92: 737-744. 10.1046/j.1365-2672.2002.01577.x.Google Scholar
- Imandi SB, Bandaru VR, Somalanka SR, Garapati HR: Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design. Enzyme Microb Tech. 2007, 40: 1367-1372. 10.1016/j.enzmictec.2006.10.012.Google Scholar
- Levinson WE, Kurtzman CP, Kuo TM: Characterization of Yarrowia lipolytica and related species for citric acid production from glycerol. Enzyme Microb Tech. 2007, 41: 292-295. 10.1016/j.enzmictec.2007.02.005.Google Scholar
- Rymowicz W, Rywinska A, Zarowska B, Juszczyk P: Citric acid production from raw glycerol by acetate mutants of Yarrowia lipolytica. Chem Pap-Chem Zvesti. 2006, 60: 391-394.Google Scholar
- Rywinska A, Rymowicz W, Zarowska B, Wojtatowicz M: Biosynthesis of Citric Acid from Glycerol by Acetate Mutants of Yarrowia lipolytica in Fed-Batch Fermentation. Food Technol Biotech. 2009, 47: 1-6.Google Scholar
- Musial I, Cibis E, Rymowicz W: Designing a process of kaolin bleaching in an oxalic acid enriched medium by Aspergillus niger cultivated on biodiesel-derived waste composed of glycerol and fatty acids. Appl Clay Sci. 2011, 52: 277-284. 10.1016/j.clay.2011.03.004.Google Scholar
- Polyols - A global strategic business report,. http://www.prweb.com/releases/polyols/sugar_alcohols_replacers/prweb8061551.htm,
- Akinterinwa O, Khankal R, Cirino PC: Metabolic engineering for bioproduction of sugar alcohols. Curr Opin Biotech. 2008, 19: 461-467. 10.1016/j.copbio.2008.08.002.Google Scholar
- Parajo JC, Dominguez H, Dominguez JM: Biotechnological production of xylitol. Part 1: Interest of xylitol and fundamentals of its biosynthesis. Bioresour Technol. 1998, 65: 191-201. 10.1016/S0960-8524(98)00038-8.Google Scholar
- Silveira MM, Jonas R: The biotechnological production of sorbitol. Appl Microbiol Biotechnol. 2002, 59: 400-408. 10.1007/s00253-002-1046-0.Google Scholar
- Song SH, Vieille C: Recent advances in the biological production of mannitol. Appl Microbiol Biotechnol. 2009, 84: 55-62. 10.1007/s00253-009-2086-5.Google Scholar
- Saha BC, Racine FM: Biotechnological production of mannitol and its applications. Appl Microbiol Biotechnol. 2011, 89: 879-891. 10.1007/s00253-010-2979-3.Google Scholar
- Moon HJ, Jeya M, Kim IW, Lee JK: Biotechnological production of erythritol and its applications. Appl Microbiol Biotechnol. 2010, 86: 1017-1025. 10.1007/s00253-010-2496-4.Google Scholar
- Soetaert W, Vanhooren PT, Vandamme EJ: Production of mannitol by fermentation. Methods in Biotechnology. 1999, 10: 261-275. 10.1007/978-1-59259-261-6_21.Google Scholar
- Bozell J, Petersen G: Technology development for the production of biobased products from biorefinery carbohydrate – US Department of Energy’s ‘Top 10’ revisited. Green Chem. 2010, 12: 539-554. 10.1039/b922014c.Google Scholar
- Huber GW, Dumesic JA: An overview of aqueous-phase catalytic processes for production of hydrogen and alkanes in a biorefinery. Catal Today. 2006, 111: 119-132. 10.1016/j.cattod.2005.10.010.Google Scholar
- Serrano-Ruiz JC, Luque R, Sepulveda-Escribano A: Transformations of biomass-derived platform molecules: from high added-value chemicals to fuels via aqueous-phase processing. Chem Soc Rev. 2011, 40: 5266-5281. 10.1039/c1cs15131b.Google Scholar
- Werpy T, Petersen G: Top value added chemicals from biomass: results of screening for potential candidates from sugars and synthesis gas. vol. 1: National Renewable Energy Lab, Golden,. 2004, http://www.osti.gov/bridge,Google Scholar
- Gallezot P: Conversion of biomass to selected chemical products. Chem Soc Rev. 2011, 41 (4): 1538-1558.Google Scholar
- Erickson B: Nelson. 2011, Perspective on opportunities in industrial biotechnology in renewable chemicals. Biotechnol J, Winters PGoogle Scholar
- Kusserow B, Schimpf S, Claus P: Hydrogenation of glucose to sorbitol over nickel and ruthenium catalysts. Adv Synth Catal. 2003, 345: 289-299. 10.1002/adsc.200390024.Google Scholar
- Prakasham R, Rao R, Hobbs P: Current trends in biotechnological production of xylitol and future prospects. Current Trends in Biotechnology and Pharmacy. 2009, 3: 8-36.Google Scholar
- Monedero V, Perez-Martinez G, Yebra MJ: Perspectives of engineering lactic acid bacteria for biotechnological polyol production. Appl Microbiol Biotechnol. 2010, 86: 1003-1015. 10.1007/s00253-010-2494-6.Google Scholar
- Yu C, Cao YJ, Zou HB, Xian M: Metabolic engineering of Escherichia coli for biotechnological production of high-value organic acids and alcohols. Appl Microbiol Biotechnol. 2011, 89: 573-583. 10.1007/s00253-010-2970-z.Google Scholar
- Kiviharju K, Nyyssola A: Contributions of biotechnology to the production of mannitol. Recent Pat Biotechnol. 2008, 2: 73-78. 10.2174/187220808784619702.Google Scholar
- Butanediol price report,. http://www.icispricing.com,
- NNFCC: Ethanol - Renewable Chemicals Factsheet,. 2010, www.nnfcc.co.uk/publications,Google Scholar
- NNDCC: Lactic Acid - Renewable Chemicals Factsheet,. 2011, www.nnfcc.co.uk/publications,Google Scholar
- NNFCC: Succinic Acid - Renewable Chemicals Factsheet. 2010Google Scholar
- Frost, Sullivan: Strategic analysis of the erythritol market. Strategic Analysis of the US Polyols Markets,. Edited by: Sullivan F. 2007, http://www.frost.com/prod/servlet/frost-home.pag,Google Scholar
- Taylor P, Fotheringham I, Wymer N, Saha B, Demirjian D, Sakaibara Y, Racine FM: Methods for Production of Xylitol in Microorganisms. USPTO, US7,960,152-B2. 2011, Biotechnology Research Development Corporation, USA, www.uspto.gov,Google Scholar
- Song KH, Baek H, Park SM, Hyun HH, Jung SR, Kim SY, Lee JK, Song JY: Candida magnoliae producing mannitol and fermentation method for producing mannitol. USPTO, US6,528,290-B1. 2003, , USA, www.uspto.govGoogle Scholar
- Dobson R, Gray V, Rumbold K: Microbial utilization of crude glycerol for the production of value-added products. J Ind Microbiol Biot. 2012, 39: 217-226. 10.1007/s10295-011-1038-0.Google Scholar
- Onishi H, Suzuki T: Microbial Production of D-Mannitol and D-Fructose from Glycerol. Biotechnol Bioeng. 1970, 12: 913-10.1002/bit.260120605.Google Scholar
- Andre A, Chatzifragkou A, Diamantopoulou P, Sarris D, Philippoussis A, Galiotou-Panayotou M, Komaitis M, Papanikolaou S: Biotechnological conversions of bio-diesel-derived crude glycerol by Yarrowia lipolytica strains. Eng Life Sci. 2009, 9: 468-478. 10.1002/elsc.200900063.Google Scholar
- Chatzifragkou A, Makri A, Belka A, Bellou S, Mavrou M, Mastoridou M, Mystrioti P, Onjaro G, Aggelis G, Papanikolaou S: Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy. 2011, 36: 1097-1108. 10.1016/j.energy.2010.11.040.Google Scholar
- ZuChem gears up for first mannitol sweetener,. http://www.foodnavigator-usa.com/Business/ZuChem-gears-up-for-first-mannitol-sweetener,
- Savergave LS, Gadre RV, Vaidya BK, Narayanan K: Strain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from Candida magnoliae mutant R23. Biochem Eng J. 2011, 55: 92-100. 10.1016/j.bej.2011.03.009.Google Scholar
- Rymowicz W, Rywinska A, Gladkowski W: Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1. Chem Pap. 2008, 62: 239-246. 10.2478/s11696-008-0018-y.Google Scholar
- Jeya M, Lee KM, Tiwari MK, Kim JS, Gunasekaran P, Kim SY, Kim IW, Lee JK: Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level. Appl Microbiol Biotechnol. 2009, 83: 225-231. 10.1007/s00253-009-1871-5.Google Scholar
- Suzuki S, Sugiyama M, Mihara Y, Hashiguchi K, Yokozeki K: Novel enzymatic method for the production of xylitol from D-arabitol by Gluconobacter oxydans. Biosci Biotech Bioch. 2002, 66: 2614-2620. 10.1271/bbb.66.2614.Google Scholar
- Sugiyama M, Suzuki S, Tonouchi N, Yokozeki K: Cloning of the xylitol dehydrogenase gene from Gluconobacter oxydans and improved production of xylitol from D-arabitol. Biosci Biotech Bioch. 2003, 67: 584-591. 10.1271/bbb.67.584.Google Scholar
- Koganti S, Loman A, Ju LK: Production and purification of arabitol from biodiesel byproduct glycerol. Abstracts of the 102nd AOCS Annual Meeting & Expo. 2011, American oil chemists’s society (AOCS), Cincinnati, Ohio, USAGoogle Scholar
- Suriyamongkol P, Weselake R, Narine S, Moloney M, Shah S: Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants - A review. Biotechnol Adv. 2007, 25: 148-175. 10.1016/j.biotechadv.2006.11.007.Google Scholar
- Posada JA, Naranjo JM, Lopez JA, Higuita JC, Cardona CA: Design and analysis of poly-3-hydroxybutyrate production processes from crude glycerol. Process Biochem. 2011, 46: 310-317. 10.1016/j.procbio.2010.09.003.Google Scholar
- Dawes EA, Senior PJ: The role and regulation of energy reserve polymers in micro-organisms. Adv Microb Physiol. 1973, 10: 135-266.Google Scholar
- Koller M, Bona R, Braunegg G, Hermann C, Horvat P, Kroutil M, Martinz J, Neto J, Pereira L, Varila P: Production of polyhydroxyalkanoates from agricultural waste and surplus materials. Biomacromolecules. 2005, 6: 561-565. 10.1021/bm049478b.Google Scholar
- Ibrahim MHA, Steinbuchel A: Zobellella denitrificans strain MW1, a newly isolated bacterium suitable for poly(3-hydroxybutyrate) production from glycerol. J Appl Microbiol. 2010, 108: 214-225. 10.1111/j.1365-2672.2009.04413.x.Google Scholar
- Grothe E, Moo-Young M, Chisti Y: Fermentation optimization for the production of poly(beta-hydroxybutyric acid) microbial thermoplastic. Enzyme Microb Tech. 1999, 25: 132-141. 10.1016/S0141-0229(99)00023-X.Google Scholar
- de Almeida A, Giordano AM, Nikel PI, Pettinari MJ: Effects of Aeration on the Synthesis of Poly(3-Hydroxybutyrate) from Glycerol and Glucose in Recombinant Escherichia coli. Appl Environ Microb. 2010, 76: 2036-2040. 10.1128/AEM.02706-09.Google Scholar
- Habe H, Fukuoka T, Kitamoto D, Sakaki K: Glycerol Conversion to D-Xylulose by a Two-stage Microbial Reaction Using Candida parapsilosis and Gluconobacter oxydans. J Oleo Sci. 2009, 58: 595-600. 10.5650/jos.58.595.Google Scholar
- Abad S, Turon X: Valorization of biodiesel derived glycerol as a carbon source to obtain added-value metabolites: Focus on polyunsaturated fatty acids. Biotechnol Adv. 2012, 30: 733-741. 10.1016/j.biotechadv.2012.01.002.Google Scholar
- Leoneti AB, Aragão-Leoneti V, de Oliveira SVWB: Glycerol as a by-product of biodiesel production in Brazil: Alternatives for the use of unrefined glycerol. Renewable Energy. 2012, 45: 138-145.Google Scholar
- Sarma SJ, Brar SK, Sydney EB, Le Bihan Y, Buelna G, Soccol CR: Microbial hydrogen production by bioconversion of crude glycerol: A review. International Journal of Hydrogen Energy. 2012, 37: 6473-6490. 10.1016/j.ijhydene.2012.01.050.Google Scholar
- Astals S, Nolla-Ardèvol V, Mata-Alvarez J: Anaerobic co-digestion of pig manure and crude glycerol at mesophilic conditions: Biogas and digestate. Bioresour Technol. 2012, 110: 63-70.Google Scholar
- Nuchdang S, Phalakornkule C: Anaerobic digestion of glycerol and co-digestion of glycerol and pig manure. J Environ Manage. 2012, 101: 164-172.Google Scholar
- Jovanovic I, Jones SB, Santosa DM, Dai Z, Ramasamy K, Zhu Y: A Survey of Opportunities for Microbial Conversion of Biomass to Hydrocarbon Compatible Fuels. PNNL-19704. 2010, Pacific Northwest National Laboratory, Richland, WA, 1-48.Google Scholar
- Nair RV, Payne MS, Trimbur DE, Valle F, Nair V, Payne S, Trimbur E: Recombinant organisms containing G3PDH and or G3P phosphatase. Derwent Innovations Index. Du Pont De Nemours & Co E I (Dupo), Genencor Int Inc (Gemv), Genencor Int (Gemv), Du Pont De Nemours&Co E I (Dupo), Derwent Primary acession number: 1999-385384
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