Improved ethanol yield and reduced Minimum Ethanol Selling Price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 1) Experimental
© Chen et al.; licensee BioMed Central Ltd. 2012
Received: 22 June 2012
Accepted: 7 August 2012
Published: 13 August 2012
Historically, acid pretreatment technology for the production of bio-ethanol from corn stover has required severe conditions to overcome biomass recalcitrance. However, the high usage of acid and steam at severe pretreatment conditions hinders the economic feasibility of the ethanol production from biomass. In addition, the amount of acetate and furfural produced during harsh pretreatment is in the range that strongly inhibits cell growth and impedes ethanol fermentation. The current work addresses these issues through pretreatment with lower acid concentrations and temperatures incorporated with deacetylation and mechanical refining.
The results showed that deacetylation with 0.1 M NaOH before acid pretreatment improved the monomeric xylose yield in pretreatment by up to 20% while keeping the furfural yield under 2%. Deacetylation also improved the glucose yield by 10% and the xylose yield by 20% during low solids enzymatic hydrolysis. Mechanical refining using a PFI mill further improved sugar yields during both low- and high-solids enzymatic hydrolysis. Mechanical refining also allowed enzyme loadings to be reduced while maintaining high yields. Deacetylation and mechanical refining are shown to assist in achieving 90% cellulose yield in high-solids (20%) enzymatic hydrolysis. When fermentations were performed under pH control to evaluate the effect of deacetylation and mechanical refining on the ethanol yields, glucose and xylose utilizations over 90% and ethanol yields over 90% were achieved. Overall ethanol yields were calculated based on experimental results for the base case and modified cases. One modified case that integrated deacetylation, mechanical refining, and washing was estimated to produce 88 gallons of ethanol per ton of biomass.
The current work developed a novel bio-ethanol process that features pretreatment with lower acid concentrations and temperatures incorporated with deacetylation and mechanical refining. The new process shows improved overall ethanol yields compared to traditional dilute acid pretreatment. The experimental results from this work support the techno-economic analysis and calculation of Minimum Ethanol Selling Price (MESP) detailed in our companion paper.
KeywordsBioethanol Pretreatment Enzymatic hydrolysis Fermentation Deacetylation Mechanical refining PFI mill
The United States consumes almost 19 million barrels of crude oil per day, of which approximately 58% is supplied by imports . The huge demand for imported oil not only increases the market price, but it is also detrimental to the U.S. economy. The Energy Information Administration (EIA) predicts that imported oil will account for 62.5% of the total domestic oil supply by 2030 . To alleviate the oil trade deficit and achieve energy independence, the production of ethanol and other transportation fuels from lignocellulosic biomass is attracting significant national interest. The National Renewable Energy Laboratory (NREL) is developing a cost-competitive bio-ethanol process using corn stover, dilute acid pretreatment and biological conversion. As disclosed in a recent techno-economic analysis report, NREL is targeting a minimum ethanol selling price (MESP) of $2.15 for this process . To meet this target, much effort has been dedicated to the optimization of this process by way of parameter-selection engineering, as well as process modification.
Pretreatment conditions for corn stover in the 2011 techno-economic analysis report
Sulfuric acid loading
22 mg/g dry biomass
Total solids loading
However, pretreatment at such conditions brings the following problems:
● The side products and degradation products such as acetic acid and furfural produced from such pretreatment strongly inhibit the microbial growth and the final yield of ethanol .
● The pretreatment reactor must be constructed of exotic metal alloys to resist corrosion at the high acid concentrations and temperatures. The estimated cost of the Incoloy 825 cladded reactor for a 2,000 ton per day capacity is about $29.9 million in direct capital costs .
● Pretreatment at such conditions requires precise residence time control. Thus, an expensive horizontal screw pretreatment reactor is required for this process.
● The pretreatment process has high chemical costs due to the usage of relatively high concentrations of sulfuric acid, which subsequently needs neutralization before enzymatic hydrolysis and fermentation, resulting in increased usage of neutralizing chemicals. Ammonium hydroxide is used in this stage because it does not introduce salts that inhibit downstream fermentation.
● The resultant ammonium salts from neutralization require remediation by wastewater treatment. Removal of the ammonium salts requires additional caustic chemicals and aerobic wastewater digestion to convert the ammonium to sodium nitrate .
To overcome these problems and further lower the MESP of cellulosic ethanol, low-severity dilute acid pretreatment processes have been investigated. Studies have shown that low-severity dilute acid pretreatment converts much less xylan to degradation products, which translates to 1) lower concentrations of fermentation inhibiting compounds; and 2) more xylan available for conversion to xylose in enzymatic hydrolysis. Lower-severity pretreatments also have broader residence time control requirements and may be able to utilize lower-cost vertical reactor configurations . Also, according to some vendors, lower acid loadings may allow the pretreatment reactor to be constructed using a lower-cost duplex stainless steel alloy.
However, low-severity dilute acid pretreatments also achieve low xylose monomer yields during pretreatment; rather, xylan is converted to higher percentages of non-fermentable oligomeric xylose. Low pretreatment severity also results in lower cellulose digestibility during enzymatic hydrolysis, which eventually increases the MESP. To increase the overall ethanol yield and lower the MESP, deacetylation and mechanical refining have been introduced and investigated in the current research to overcome the limitations of lower-severity dilute acid pretreatments.
Deacetylation is a unit operation designed to selectively remove acetyl groups from the hemicellulosic fraction of the feedstock prior to pretreatment [6, 7]. It decreases the concentration of acetic acid released during pretreatment, which minimizes the inhibition effect of acetate salts on the downstream fermentation. Historically, acetate has been removed after pretreatment using ion exchange technologies; however, this has proven to be quite costly . With the application of an alkaline extraction stage, separation of acetic acid from deacetylated feedstock may require only a supplemental dewatering or filtration step. In addition, recent studies have found that deacetylation not only significantly improves the downstream fermentability of the sugar hydrolysates but also increases sugar yields in pretreatment and enzymatic hydrolysis [6, 8]. Therefore, deacetylation of the feedstock before low-severity pretreatment by alkaline extraction could potentially improve overall ethanol yield compared to standard high severity pretreatment techniques.
Mechanical refining has been widely applied in the pulp and paper industry to improve the bonding ability of the fiber and increase paper strength. It has also been used to generate microfibrils, shorten long fibers, and develop the fiber’s absorbency and porosity . Disc refining has also been applied by Zhu et al. after the sulfite pretreatment of biomass to improve sugar yield during enzymatic hydrolysis . Mild effects on saccharification by disc refining have been reported . However, a recent study by Koo et al. reported that by applying PFI refining technology to green liquor-treated hardwood, enzymatic conversion was significantly improved, while enzyme usage was reduced by up to 50% . The contradictory results are possibly due to: 1) the variation in pretreatment type and conditions, which result in different substrates; 2) the mechanical refining mechanism, because different types of refiners have been utilized; and 3) the degree of mechanical refining. Investigation of these factors has been conducted in NREL and will be reported in the future. In the current study, mechanical refining in a PFI mill has been applied to further improve the digestibility of deacetylated and lower-severity-pretreated corn stover. The PFI mill, although a laboratory mechanical refiner, provides accurate control and induces homogenous refining effects on substrate. A PFI mill is essentially a compression unit, which, given the same energy input, produces refining effect differing significantly from an conventional disc refiner . Exerting more force as compression rather than shear, results in higher internal fibrillation and lower external fibrillation and fiber shortening . It is also reported that during the PFI refining of never-dried softwood pulp, internal surface area of softwood fibers increased by up to 10% . Similar pore volume improvement of pulp by PFI refining has also been reported by others [14, 15] Many researchers have also shown internal surface area to be one of the key factors limiting biomass digestibility [16–20]. At NREL, attempts are underway to scale up the PFI mill using a Szego mill developed by Dr. Olive Trass in Toronto University. Results of these efforts will be reported in the future. For this study, acid loading and temperature in pretreatment were reduced to 0.5% (w/w) H2SO4 and 150°C, respectively. The effect of deacetylation and mechanical refining on enzymatic hydrolysis was explored at low and high solids loadings as well as on washed and unwashed substrates. Additionally, the effect of deacetylation on fermentation was studied in pH-controlled fermentations. Finally, the ethanol yields per ton of dry biomass from different pretreatment options were compared and summarized. This paper provides experimental results supporting the techno-economic analysis (TEA) in our companion paper .
Results and discussion
Xylan mass closure of pretreatment at 150°C, 8 mg H 2 SO 4 per g of O.D. corn stover for 20 min
Corn stover type
Xylan conversion during steam gun pretreatment
Xylose degraded into furfural (%)
Xylan in solids (%)
Mass closure (%)
Low-solids enzymatic hydrolysis with washed solids
The controls with CT20 were also compared with deacetylated (De-Ac) samples at the same enzyme loadings. Deacetylation increased glucose yields by 7% ~ 10% and xylose yields by 18% ~ 21%. The large monomeric xylose release indicates the preference of hemicellulase enzymes for deacetylated xylan, as discussed previously in the literature [7, 8]. The improved xylan removal further benefits the conversion of glucose.
As in the other digestions, pretreated corn stover exhibited increased glucan and xylan conversion after PFI refining. For control Kramer 34 M95, glucan conversion at CT10 using PFI milled pretreated corn stover (PCS) and CT20 using unrefined PCS were very similar, as minimal yield losses were observed with a 50% reduction in enzyme loading. For control Kramer 33B51, the glucose yield was improved by approximately 15% in the same case. This is very good evidence that substrate accessibility is a key limiting factor in the extent of enzymatic conversion. The size reduction resulting from the PFI refining process appears to increase the reactive surface area of the biomass, presumably exposing more substrate to the enzymes. This suggests that the slowdown and failure to release 100% of the sugars in the non-PFI-refined samples is at least partially due to a lack of enzyme accessibility to the substrate and not to a chemical barrier inherent to biomass composition. Interestingly, the xylan conversion extents for the CT10 and CT20 loadings were practically identical with refined corn stover, but much higher than with the unrefined stover. This suggests that the PFI refining has a greater effect on the digestibility of hemicellulose and that increased hemicellulose removal may be responsible for the increased digestion of the cellulose as discussed previously. The deacetylated stover varieties achieved higher conversions than the native stover did, suggesting that feedstock acetylation is an issue that must be addressed even after mechanical refining. Deacetylated PCS that was PFI-refined achieved close to 100% glucose conversion and 95% xylose conversion for both corn stover varieties, showing that carbohydrate accessibility in biomass and xylan-acetyl bond cleavage are two critical keys for the improvement of enzymatic hydrolysis yields.
High-solids enzymatic hydrolysis with washed solids
The effects of deacetylation and mechanical refining on high-solids enzymatic hydrolysis with washed pretreated solids are very similar to the effects shown in low solids enzymatic hydrolysis; however, all yields are 5% ~ 10% lower. This is likely due to the high initial solids content limiting the flow of contained water, thus inhibiting enzyme diffusion and lowering hydrolysis yields.
Deacetylation again is shown to improve digestibility. An average yield improvement of ~10% for glucose and ~15% for xylose was achieved with the incorporation of the deacetylation process. Both deacetylated corn stover varieties showed glucose yields over 80% and xylose yields over 75%, while the yields for native corn stover samples were in the range of 69% ~ 73% (glucose) and 55% ~ 58% (xylose).
Mechanical refining also was demonstrated to improve digestibility. Samples with mechanical refining but without deacetylation achieved ~85% glucose yields and about 75% xylose yields. These were 10% ~ 15% and 15% ~ 20% improvements over the unrefined controls for glucose and xylose yield, respectively.
The highest glucose and xylose yields occurred in corn stover samples that were both deacetylated and mechanically refined. Under these conditions, both corn stover varieties achieved over 90% yields for both glucose and xylose.
In general, results from enzymatic hydrolysis at a 20% solids loading follow the same trend as was found in low-solids experiments. Applying either deacetylation or mechanical refining improved glucose yields by 10% ~ 15% and xylose yields by 15% ~ 20%. The glucose yields increased by 15% ~ 20% and xylose yields increased by 25% ~ 30% when deacetylated corn stover was pretreated and mechanically refined.
High-solids enzymatic hydrolysis with unwashed solids
In all cases, very little monomeric xylose was produced during enzymatic hydrolysis. The high xylose concentration in the background liquor likely inhibited further hydrolysis of xylan in the solids or xylo-oligomers in the liquor. The high sugar product concentration can also stop the hydrolysis reaction thermodynamically by product inhibition.
The reduced xylose conversion also led to lower glucose yields when compared to results using washed PCS. The glucose yields for native control samples fall between 57% and 65%. Glucose yields for deacetylated samples range from 65% to 71%. Mechanical refining consistently improved glucose yields by more than 10% over unrefined samples. However, all yields are 5% ~ 10% lower than the yields for washed pretreated solids.
Ethanol yield from one ton of biomass
Comparison of ethanol yield per dry ton of biomass for cases with and without washed solid process options, Ctrl = Control, DA = Deacetylated, MR = Mechanical Refining
Ethanol yield (gal/dry ton biomass)
4)DA + MR
8)DA + MR
Ethanol yields in cases 4, 6, and 8 all exceed the 2011 design case, and case 7 nearly matches it. All these process scenarios include deacetylation and/or mechanical refining, offering an indication of the value of deacetylation and mechanical refining in a low-severity dilute acid pretreatment process. The hydrolysate wash stage has also proven to have a large role in maximizing ethanol yield. However, the overall cost of implementing these technologies on the commercial scale is unknown. In order for these scenarios to be practically included in the cellulosic ethanol design case, the increased ethanol yield must offset the additional capital and operational costs. This issue is addressed in our companion paper , where a techno-economic analysis is performed using the data developed in this study.
Low-severity pretreatment at reduced temperatures and acid loadings is one way to mitigate these effects. However, low-severity pretreatment historically suffers from low monomeric xylose yields and cellulose digestibility. To solve these problems, deacetylation, mechanical disk refining, and solids washing were investigated and tested in numerous combinations using two varieties of corn stover.
Deacetylation is shown to increase xylose monomer yield during pretreatment but also to improve insoluble solids digestibility. Mechanical refining also has a positive impact on glucose and xylose yields during enzymatic hydrolysis. Combining both unit operations with low-severity pretreatment resulted in glucose yields of over 90% in low-solids enzymatic hydrolysis and in high-solids enzymatic hydrolysis using washed solids.
The washing of pretreated solids was also shown to be critical to the enzymatic conversion of residual xylan in the pretreated solids.
The reduction in acetic acid concentration from deacetylation in combination with lower pretreatment severity also improved the xylose utilization and ethanol yield in fermentation, ultimately achieving over 90% ethanol yield in less than 30 hours.
Deacetylation, mechanical refining, and solids washing has proven to be effective in improving ethanol yield. However, the commercial-scale viability of these additional unit operations needs to be evaluated to confirm that the resultant yield increases offset the increased capital and operational costs they present. In our companion paper, data provided by these experiments is used to perform a techno-economic analysis to evaluate the process economics, and the impact of these process modifications on the MESP is discussed .
In this study, two different varieties of corn stover feedstock were used. Those were from the Kramer farm in Wray, Colorado (Pioneer varieties 34 M95 and 33B51).
Deacetylation of the corn stover was performed by alkali extraction in a 200 L Recirculating Atmospheric Pressure Impregnation (RAPI) system. Approximately 10 kg of dry (~93 wt% solids), ½-inch (34 M95, 33B51) knife-milled corn stover was loaded into Hastelloy C-276 wire mesh (20 mesh screen) baskets and immersed in the recirculation bath containing 120 L of 0.4 wt% NaOH (0.1 M) solution at 70°C to 80°C for 3 hours. The initial pH was approximately 12. The solid-to-liquid ratio was 1:12. The recirculation helps to distribute alkali solution evenly throughout the basket and helps to provide efficient extraction of the corn stover feedstock. After extraction, the excess alkali solution was drained and a sample was retained for pH, acetate, and sugar concentration measurements. The alkali-extracted corn stover was washed with four separate washes (120 L) of warm water (~60°C) for 1 h, with the basket drained to ~15 wt% solids in between each wash, until the pH of the washed liquor measured ~8.0. The alkali-extracted and washed feedstock was used for dilute acid soaking as described below.
Acid impregnation was carried out using the same 200-L recirculating (RAPI) system as described above. Approximately 120 L of warm (48°C to 50°C), 0.5 wt% H2SO4 was prepared beforehand in the recirculation tank. A Hastelloy C-276 wire mesh (20 mesh screen) basket was loaded with 50 kg of native or deacetylated and washed corn stover feedstock and lowered into the warm (48°-50°C) dilute acid bath for 3 hours with recirculation of the acid. Following acid impregnation, the feedstock in the basket was drained of excess acid to approximately 20% solids and loaded into the mold of a hydraulic dewatering press, where the acid impregnated feedstock was pressed to ~40% to 48% solids.
The 4-L NREL Digester (steam explosion reactor) was used for all pretreatment experiments. The NREL Digester was pre-heated to pretreatment temperature and then was loaded with 500.0 g of acid-impregnated and pressed feedstock and quickly heated (~5 to 10 sec) via direct steam injection to reaction temperature. Pretreatments were carried out at the lower severity conditions of 150°C, 0.5 wt% H2SO4, and 20 min residence times. The initial solids concentration of the acid impregnated and dewatered corn stover is approximately 40%.
For mechanically refined samples, the biomass slurry was processed in a PFI refiner and run for 4,000 revolutions. This process significantly reduces the particle size of the solids. Mechanical refining was carried out in collaboration with Dr. Sunkyu Park at North Carolina State University using their bench-scale PFI refining mill. The pretreated biomass slurries were diluted to 20 wt% consistency and refined for a total of 4,000 revolutions at room temperature. Around 40 g O.D. dry material were refined in a single batch. The solids were not washed before refining.
Novozymes (Frankinton, NC) cellulases, CTec2, and hemicellulases, HTec2, were used in the current study. The enzyme loadings were added at varied levels as described in the results and discussion section.
For washed solids enzymatic hydrolysis, the pretreated slurry was thoroughly washed with water in centrifuge bottles until xylose was <0.01 g/L (measured by YSI), usually 6 times.
Low-solids enzymatic hydrolysis experiments were set up in 125 mL Erlenmeyer flasks containing 50 mM citrate buffer, pH 4.8, enzyme, and 1.0% glucan loading (w/v). Digestion conditions were 150 rpm at 50°C.
High-solids enzymatic hydrolysis experiments were performed in 125 mL wide-mouth polypropylene bottles (Thermo Fisher Scientific, Inc., Waltham, MA) loaded with 60 g of washed slurry at 20% insoluble solids. Novozyme CTec2 is loaded at 20 mg protein per g of cellulose, while Novozyme HTec2 is loaded at 2 mg protein per g of cellulose. The roller bottle saccharification reactors employ gravitational tumbling as a mixing mechanism to homogenize solids by horizontally rotating the reaction vessels at 4 rpm on a 3-deck roller apparatus for mini bottles (Wheaton Industries Inc., Millville, NJ). The roller apparatus was placed in a general purpose incubator (Model 1545, VWR International, LLC, West Chester, PA) for temperature control at 48.5°C.
In whole slurry enzymatic hydrolysis, PCS was adjusted to a pH of 5.0 by adding small amounts of ammonium hydroxide and mixing well. No citrate buffer was added due to the inhibitory effect of citrate ions on Zymomonas mobilis growth and fermentation. Saccharification experiments were carried out in 125 mL wide-mouth polypropylene roller bottles with pH-adjusted slurry at 25% total solids. The other conditions are the same as for high-solids enzymatic hydrolysis using washed solids.
Microorganism and revive/pre-seed culture
Zymomonas mobilis strain 8b was used in this evaluation. The strain was taken from cell stock stored at −70°C. The pre-seed medium consisted of 10 g/L yeast extract and 2 g/L potassium phosphate monobasic (1X RM), supplemented with 100 g/L glucose and 20 g/L xylose. The reviving culture was started by transferring 1 mL of Z. mobilis cell stock into 9 mL of pre-seed medium in a 15 mL tube. The culture was incubated at 33°C with no agitation. The culture was sampled at 8 hours for an optical density reading at 600 nm. The pre-seed culture was used to inoculate the batch seed fermenter with media composition of RM (1X), 150 g/L glucose; 20 g/L xylose; and 1 g/L sorbitol. pH and temperature were controlled at 5.8 by KOH (4 N) and at 33°C.
Fermentation experiments to evaluate the neutralized saccharified whole slurry were performed in BioStat-Q Plus fermenters at a 300 mL working volume using rZ.mobilis strain 8b. Rich media consisting of 10 g/L yeast extract and 2 g/L KH2PO4 was added to enzymatically-hydrolyzed whole slurry. The fermenters were inoculated at an optical density (@ 600 nm) of approximately 1.0 absorbance units using a direct transfer procedure (10% v/v). The fermentation was conducted at a temperature of 33°C, a pH of 5.8 (controlled with 4 M KOH), and an agitation speed of 300 rpm. The fermentation was typically finished in 72 h. Ethanol yield calculations were based on initial glucose, xylose, and fructose concentrations and differences between initial and final ethanol concentrations.
Chemical analysis and yield calculations
Pretreatment and enzymatic saccharification liquors and fermentation samples were analyzed using HPLC according to standard NREL laboratory analytical procedures (LAPs) . Solid residues were also analyzed in accordance with the published NREL LAPs [26, 27]. Sugar yields from high solids enzymatic hydrolysis were calculated using the equations developed by Zhu et al. .
In the liquor phase of pretreated slurry, acetate is present in two forms: 1) free acetate/acetic acid, released from the xylan; and 2) acetyl groups covalently bound to the dissolved xylan oligomers. Free acetate is measured by direct injection on a Shodex SP0810 acid column (Kawasaki, Japan), while total acetate is measured with the same column after a 4% acid hydrolysis of the filtered pretreatment liquid that contains all solubilized compounds. The 4% acid hydrolysis at 121°C for 1 h hydrolyzes the remaining acetyl groups covalently bound to the xylooligomers.
Particle size analysis
The particle size of biomass samples was measured using laser diffraction on a Mastersizer 2000 with the Hydro 2000 G module (Malvern Instruments). The instrument measures particle sizes over the range from 0.02 to 2000 μm in a recirculating liquid suspension. For the analysis, 0.05 to 0.2 g of each cellulose sample was dispersed in water in a 15-mL centrifuge tube. Thereafter, individual dispersed samples were vortex mixed and transferred to the Hydro 2000 G module that contained 0.8 – 1.0 L of deionized water (nr = 1.33 at 20°C), with a stirrer setting of 600 RPM and a pump setting of 1250 RPM. After 30 seconds delay, three, 15 second readings (30 seconds apart) of the circulating samples were acquired and averaged. The volume weighted mean value was used to represent the mean particle diameter (MPD). Each sample was run in triplicate and MPD is shown as the average of the triplicates.
Dr. Xiaowen Chen received his master’s and Ph.D’s degree in chemical engineering from University of Maine. He is now a chemical engineer at the National Renewable Energy Lab. His research interest is in process development and biochemical engineering in cellulosic ethanol.
Minimum ethanol selling price
National renewable energy laboratory
Energy information administration
Techno economic analysis
Pretreated corn stover
Enzymatic hydrolysis with CTec2 10 mg protein /g of cellulose and HTec2 1 mg protein /g of cellulose loading
Enzymatic hydrolysis CTec2 20 mg protein /g of cellulose and HTec2 2 mg protein /g of cellulose loading
Enzymatic hydrolysis CTec2 40 mg protein /g of cellulose and HTec2 4 mg protein /g of cellulose loading
Enzymatic hydrolysis CTec2 60 mg protein /g of cellulose and HTec26 mg protein /g of cellulose loading
Recirculating atmospheric pressure impregnation
Mean particle diameter.
We would like to acknowledge the funding support from Office of Biomass Program (OBP), a program in DOE EERE (Contract no. DE-AC36-08GO28308). We also want to thank the Biomass Analytical Team for their technical assistance and analysis.
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