Differential expression of genes involved in (A) the fatty acid biosynthesis; (B) triacylglycerol biosynthesis; (C)
β-oxidation; and (D) starch biosynthesis. Pathway were reconstructed based on the de novo assembly and quantitative annotation of the N. oleoabundans transcriptome. (A) Enzymes include: ACC, acetyl-CoA carboxylase (EC: 18.104.22.168); MAT, malonyl-CoA ACP transacylase (EC: 22.214.171.124); KAS, beta-ketoacyl-ACP synthase (KAS I, EC: 126.96.36.199; KASII, EC: 188.8.131.52; KAS III, EC: 184.108.40.206); KAR, beta-ketoacyl-ACP reductase (EC: 220.127.116.11); HAD, beta-hydroxyacyl-ACP dehydrase (EC: 4.2.1.-); EAR, enoyl-ACP reductase (EC: 18.104.22.168); AAD, acyl-ACP desaturase (EC: 22.214.171.124); OAH, oleoyl-ACP hydrolase (EC: 126.96.36.199); FatA, Acyl-ACP thioesterase A (EC: 3.1.2.-); Δ12D, Δ12(ω6)-desaturase (EC: 188.8.131.52); Δ15D, Δ15(ω3)-desaturase (EC: 1.4.19.-); (B) Enzymes include: GK, glycerol kinase (EC: 184.108.40.206); GPAT, glycerol-3-phosphate O-acyltransferase (EC: 220.127.116.11); AGPAT, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase (EC:18.104.22.168); PP, phosphatidate phosphatase (EC: 22.214.171.124); DGAT, diacylglycerol O-acyltransferase (EC: 126.96.36.199); and PDAT, phopholipid:diacyglycerol acyltransferase (EC 188.8.131.52); (C) Enzymes include: ACS, acyl-CoA synthetase (EC: 184.108.40.206); ACOX1, acyl-CoA oxidase (EC: 220.127.116.11); ECH, enoly-CoA hydratase (EC: 18.104.22.168); HADH, 3-hydroxyacyl-CoA dehydrogenase (EC: 22.214.171.124); ACAT, acetyl-CoA C-acetyltransferase (EC: 126.96.36.199, 188.8.131.52); (D) Enzymes include: PGM, phosphoglucomutase (EC: 184.108.40.206); AGPase, ADP-glucose pyrophosphorylase (EC: 220.127.116.11); SS, starch synthase (EC: 18.104.22.168); BE, α-1,4-glucan branching enzyme (EC: 22.214.171.124); and HXK, hexokinase (126.96.36.199). Starch catabolism enzymes include: α-AMY, α-amylase (EC: 188.8.131.52); O1,6G, oligo-1,6-glucosidase (EC: 184.108.40.206); β-AMY, β-amylase (EC: 220.127.116.11); and SPase, starch phosphorylase (EC: 18.104.22.168). Ethanol fermentation via pyruvate enzymes include: PDC, pyruvate decarboxylase (EC: 22.214.171.124); and ADH, alcohol dehydrogenase (EC: 126.96.36.199). Enzymes aceE, pyruvate dehydrogenase E1 component (EC 188.8.131.52); aceF, pyruvate dehydrogenase E2 component (EC: 184.108.40.206); and pdhD, dihydrolipoamide dehydrogenase (EC 220.127.116.11), transforms pyruvate into acetyl-CoA. Key enzymes are shown with an asterisk (*) next to the enzyme abbreviations, and dashed arrows denote reaction(s) for which the enzymes are not shown. All presented fold changes are statistically significant, q value < 0.05.