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  1. Research

    Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production

    The high crystallinity of cellulosic biomass myofibrils as well as the complexity of their intermolecular structure is a significant impediment for biofuel production. Cloning of celB-, celC-encoded cellulases (c...

    Eman Ibrahim, Kim D. Jones, Keith E. Taylor, Ebtesam N. Hosseney, Patrick L. Mills and Jean M. Escudero

    Biotechnology for Biofuels 2017 10:52

    Published on: 27 February 2017

  2. Research

    Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in Saccharomyces cerevisiae

    Cellulase expression via extracellular secretion or surface display in Saccharomyces cerevisiae is one of the most frequently used strategies for a consolidated bioprocess (CBP) of cellulosic ethanol production. ...

    Hongting Tang, Meihui Song, Yao He, Jiajing Wang, Shenghuan Wang, Yu Shen, Jin Hou and Xiaoming Bao

    Biotechnology for Biofuels 2017 10:53

    Published on: 27 February 2017

  3. Research

    A process for producing lignin and volatile compounds from hydrolysis liquor

    Hot water hydrolysis process is commercially applied for treating wood chips prior to pulping or wood pellet production, while it produces hydrolysis liquor as a by-product. Since the hydrolysis liquor is dilu...

    Tooran Khazraie, Yiqian Zhang, Dmitry Tarasov, Weijue Gao, Jacquelyn Price, Nikolai DeMartini, Leena Hupa and Pedram Fatehi

    Biotechnology for Biofuels 2017 10:47

    Published on: 23 February 2017

  4. Research

    Surface properties correlate to the digestibility of hydrothermally pretreated lignocellulosic Poaceae biomass feedstocks

    Understanding factors that govern lignocellulosic biomass recalcitrance is a prerequisite for designing efficient 2nd generation biorefining processes. However, the reasons and mechanisms responsible for quant...

    Demi T. Djajadi, Aleksander R. Hansen, Anders Jensen, Lisbeth G. Thygesen, Manuel Pinelo, Anne S. Meyer and Henning Jørgensen

    Biotechnology for Biofuels 2017 10:49

    Published on: 23 February 2017

  5. Research

    Type-dependent action modes of TtAA9E and TaAA9A acting on cellulose and differently pretreated lignocellulosic substrates

    Lytic polysaccharide monooxygenase (LPMO) is a group of recently identified proteins that catalyze oxidative cleavage of the glycosidic linkages of cellulose and other polysaccharides. By utilizing the oxidati...

    In Jung Kim, Nari Seo, Hyun Joo An, Jae-Han Kim, Paul V. Harris and Kyoung Heon Kim

    Biotechnology for Biofuels 2017 10:46

    Published on: 22 February 2017

  6. Research

    Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast

    Assembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both commodity chemicals (including biofuels) and high-value chemicals. Promot...

    Bingyin Peng, Manuel R. Plan, Alexander Carpenter, Lars K. Nielsen and Claudia E. Vickers

    Biotechnology for Biofuels 2017 10:43

    Published on: 21 February 2017

  7. Research

    Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

    Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorg...

    Daochen Zhu, Peipei Zhang, Changxiao Xie, Weimin Zhang, Jianzhong Sun, Wei-Jun Qian and Bin Yang

    Biotechnology for Biofuels 2017 10:44

    Published on: 21 February 2017

  8. Research

    A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance of Synechocystis sp. PCC6803 to 1-butanol possibly via promoter-directed transcriptional silencing

    Microbial small RNAs (sRNAs) have been proposed as valuable regulatory elements for optimizing cellular metabolism for industrial purposes. However, little information is currently available on functional rele...

    Tao Sun, Guangsheng Pei, Jiangxin Wang, Lei Chen and Weiwen Zhang

    Biotechnology for Biofuels 2017 10:42

    Published on: 20 February 2017

  9. Research

    Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments

    The capacity of fungi, such as Aspergillus niger, to degrade lignocellulose is harnessed in biotechnology to generate biofuels and high-value compounds from renewable feedstocks. Most feedstocks are currently pre...

    Paul Daly, Jolanda M. van Munster, Martin J. Blythe, Roger Ibbett, Matt Kokolski, Sanyasi Gaddipati, Erika Lindquist, Vasanth R. Singan, Kerrie W. Barry, Anna Lipzen, Chew Yee Ngan, Christopher J. Petzold, Leanne Jade G. Chan, Steven T. Pullan, Stéphane Delmas, Paul R. Waldron…

    Biotechnology for Biofuels 2017 10:35

    Published on: 7 February 2017

  10. Research

    A versatile 2A peptide-based bicistronic protein expressing platform for the industrial cellulase producing fungus, Trichoderma reesei

    The industrial workhorse fungus, Trichoderma reesei, is typically exploited for its ability to produce cellulase enzymes, whereas use of this fungus for over-expression of other proteins (homologous and heterolog...

    Venkataramanan Subramanian, Logan A. Schuster, Kyle T. Moore, Larry E. Taylor, John O. Baker, Todd A. Vander Wall, Jeffrey G. Linger, Michael E. Himmel and Stephen R. Decker

    Biotechnology for Biofuels 2017 10:34

    Published on: 6 February 2017

  11. Research

    Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries

    Lignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount of lignin is produced as a by-product of the paper industry, while cellulosic componen...

    Barindra Sana, Kuan Hui Burton Chia, Sarada S. Raghavan, Balamurugan Ramalingam, Niranjan Nagarajan, Jayasree Seayad and Farid J. Ghadessy

    Biotechnology for Biofuels 2017 10:32

    Published on: 3 February 2017

  12. Research

    Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

    Glycoside hydrolases (GHs) are enzymes that hydrolyze polysaccharides into simple sugars. To better understand the specificity of enzyme hydrolysis within the complex matrix of polysaccharides found in the pla...

    Johnnie A. Walker, Sivakumar Pattathil, Lai F. Bergeman, Emily T. Beebe, Kai Deng, Maryam Mirzai, Trent R. Northen, Michael G. Hahn and Brian G. Fox

    Biotechnology for Biofuels 2017 10:31

    Published on: 2 February 2017

  13. Research

    Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

    During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved ove...

    Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota, Gustavo Henrique Goldman, Markku Saloheimo, Vera Sacon and Matti Siika-aho

    Biotechnology for Biofuels 2017 10:30

    Published on: 2 February 2017

  14. Research

    Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation

    Nitrogen starvation and limitation are known to induce important physiological changes especially in lipid metabolism of microalgae (triglycerides, membrane lipids, beta-carotene, etc.). Although little inform...

    Hubert Bonnefond, Nina Moelants, Amélie Talec, Patrick Mayzaud, Olivier Bernard and Antoine Sciandra

    Biotechnology for Biofuels 2017 10:25

    Published on: 31 January 2017

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