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  1. Research

    Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

    Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by...

    Ioannis Papapetridis, Marlous van Dijk, Antonius J. A. van Maris and Jack T. Pronk

    Biotechnology for Biofuels 2017 10:107

    Published on: 26 April 2017

  2. Research

    Moderate alkali-thermophilic ethanologenesis by locally isolated Bacillus licheniformis from Pakistan employing sugarcane bagasse: a comparative aspect of aseptic and non-aseptic fermentations

    Biofuels obtained from first-generation (1G) sugars-starch streams have been proven unsustainable as their constant consumption is not only significantly costly for commercial scale production systems, but it ...

    Qurat-ul-Ain Ahmad, Shang-Tian Yang, Maleeha Manzoor and Javed Iqbal Qazi

    Biotechnology for Biofuels 2017 10:105

    Published on: 24 April 2017

  3. Research

    The influence of day/night cycles on biomass yield and composition of Neochloris oleoabundans

    Day/night cycles regulate the circadian clock of organisms to program daily activities. Many species of microalgae have a synchronized cell division when grown under a day/night cycle, and synchronization migh...

    Lenneke de Winter, Iago Teles Dominguez Cabanelas, Dirk E. Martens, René H. Wijffels and Maria J. Barbosa

    Biotechnology for Biofuels 2017 10:104

    Published on: 22 April 2017

  4. Research

    Oxidation of a non-phenolic lignin model compound by two Irpex lacteus manganese peroxidases: evidence for implication of carboxylate and radicals

    Manganese peroxidase is one of the Class II fungal peroxidases that are able to oxidize the low redox potential phenolic lignin compounds. For high redox potential non-phenolic lignin degradation, mediators su...

    Xing Qin, Xianhua Sun, Huoqing Huang, Yingguo Bai, Yuan Wang, Huiying Luo, Bin Yao, Xiaoyu Zhang and Xiaoyun Su

    Biotechnology for Biofuels 2017 10:103

    Published on: 21 April 2017

  5. Research

    In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood

    Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and “green” chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulo...

    Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J. Jönsson and Ewa J. Mellerowicz

    Biotechnology for Biofuels 2017 10:98

    Published on: 20 April 2017

  6. Research

    Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum

    Lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. The production of these enzymes is transcriptionally regulated by multiple tra...

    Liwei Gao, Zhonghai Li, Chengqiang Xia, Yinbo Qu, Meng Liu, Piao Yang, Lele Yu and Xin Song

    Biotechnology for Biofuels 2017 10:100

    Published on: 20 April 2017

  7. Research

    Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum

    Microalgae have emerged as a potential feedstock for biofuels and bioactive components. However, lack of microalgal strains with promising triacylglycerol (TAG) content and desirable fatty acid composition hav...

    Srinivasan Balamurugan, Xiang Wang, Hong-Lei Wang, Chun-Jing An, Hui Li, Da-Wei Li, Wei-Dong Yang, Jie-Sheng Liu and Hong-Ye Li

    Biotechnology for Biofuels 2017 10:97

    Published on: 20 April 2017

  8. Research

    Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar

    Biomass pretreatment remains an essential step in lignocellulosic biofuel production, largely to facilitate the efficient removal of lignin and increase enzyme accessibility to the polysaccharides. In recent y...

    Kwang Ho Kim, Tanmoy Dutta, John Ralph, Shawn D. Mansfield, Blake A. Simmons and Seema Singh

    Biotechnology for Biofuels 2017 10:101

    Published on: 20 April 2017

  9. Research

    Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation

    Despite being the most important cellulase producer, the cellulase-regulating carbon source signal transduction processes in Trichoderma reesei are largely unknown. Elucidating these processes is the key for unve...

    Mingyu Wang, Meiling Zhang, Ling Li, Yanmei Dong, Yi Jiang, Kuimei Liu, Ruiqin Zhang, Baojie Jiang, Kangle Niu and Xu Fang

    Biotechnology for Biofuels 2017 10:99

    Published on: 20 April 2017

  10. Research

    Genome-wide search for candidate genes for yeast robustness improvement against formic acid reveals novel susceptibility (Trk1 and positive regulators) and resistance (Haa1-regulon) determinants

    Formic acid is an inhibitory compound present in lignocellulosic hydrolysates. Understanding the complex molecular mechanisms underlying Saccharomyces cerevisiae tolerance to this weak acid at the system level is...

    Sílvia F. Henriques, Nuno P. Mira and Isabel Sá-Correia

    Biotechnology for Biofuels 2017 10:96

    Published on: 19 April 2017

  11. Research

    Regional water footprints of potential biofuel production in China

    Development of biofuels is considered as one of the important ways to replace conventional fossil energy and mitigate climate change. However, rapid increase of biofuel production could cause other environment...

    Xiaomin Xie, Tingting Zhang, Liming Wang and Zhen Huang

    Biotechnology for Biofuels 2017 10:95

    Published on: 18 April 2017

  12. Research

    Lipid production and molecular dynamics simulation for regulation of accD gene in cyanobacteria under different N and P regimes

    Microalgae grown under different nutrient deficient conditions present a good source of natural lipids with applications for several types of biofuels. The expression of acetyl-CoA carboxylase gene can further...

    Roshan Kumar, Koushik Biswas, Puneet Kumar Singh, Pankaj Kumar Singh, Sanniyasi Elumalai, Pratyoosh Shukla and Sunil Pabbi

    Biotechnology for Biofuels 2017 10:94

    Published on: 17 April 2017

  13. Research

    Rescuing ethanol photosynthetic production of cyanobacteria in non-sterilized outdoor cultivations with a bicarbonate-based pH-rising strategy

    Ethanol photosynthetic production based on cyanobacteria cell factories utilizing CO2 and solar energy provides an attractive solution for sustainable production of green fuels. However, the scaling up processes ...

    Zhi Zhu, Guodong Luan, Xiaoming Tan, Haocui Zhang and Xuefeng Lu

    Biotechnology for Biofuels 2017 10:93

    Published on: 14 April 2017

  14. Research

    Enhancement of ethanol production from green liquor–ethanol-pretreated sugarcane bagasse by glucose–xylose cofermentation at high solid loadings with mixed Saccharomyces cerevisiae strains

    Efficient cofermentation of glucose and xylose is necessary for economically feasible bioethanol production from lignocellulosic biomass. Here, we demonstrate pretreatment of sugarcane bagasse (SCB) with green...

    Yanzhi You, Pengfei Li, Fuhou Lei, Yang Xing and Jianxin Jiang

    Biotechnology for Biofuels 2017 10:92

    Published on: 13 April 2017

  15. Research

    Functional photosystem I maintains proper energy balance during nitrogen depletion in Chlamydomonas reinhardtii, promoting triacylglycerol accumulation

    Nutrient deprivation causes significant stress to the unicellular microalga, Chlamydomonas reinhardtii, which responds by significantly altering its metabolic program. Following N deprivation, the accumulation of...

    Mahmoud Gargouri, Philip D. Bates, Jeong-Jin Park, Helmut Kirchhoff and David R. Gang

    Biotechnology for Biofuels 2017 10:89

    Published on: 13 April 2017

  16. Research

    Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii

    Nutrient limitation, such as nitrogen depletion, is the most widely used method for improving microalgae fatty acid production; however, these harsh conditions also inhibit algal growth significantly and even ...

    Chaogang Wang, Xi Chen, Hui Li, Jiangxin Wang and Zhangli Hu

    Biotechnology for Biofuels 2017 10:91

    Published on: 13 April 2017

  17. Research

    Multi-product biorefineries from lignocelluloses: a pathway to revitalisation of the sugar industry?

    Driven by a range of sustainability challenges, e.g. climate change, resource depletion and expanding populations, a circular bioeconomy is emerging and expected to evolve progressively in the coming decades. ...

    Somayeh Farzad, Mohsen Ali Mandegari, Miao Guo, Kathleen F. Haigh, Nilay Shah and Johann F. Görgens

    Biotechnology for Biofuels 2017 10:87

    Published on: 11 April 2017

  18. Research

    Bioprocessing of tea oil fruit hull with acetic acid organosolv pretreatment in combination with alkaline H2O2

    As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, includi...

    Song Tang, Rukuan Liu, Fubao Fuelbiol Sun, Chunying Dong, Rui Wang, Zhongyuan Gao, Zhanying Zhang, Zhihong Xiao, Changzhu Li and Hui Li

    Biotechnology for Biofuels 2017 10:86

    Published on: 8 April 2017

  19. Research

    Robust, high-productivity phototrophic carbon capture at high pH and alkalinity using natural microbial communities

    Bioenergy with carbon capture and storage (BECCS) has come to be seen as one of the most viable technologies to provide the negative carbon dioxide emissions needed to constrain global temperatures. In practic...

    Christine E. Sharp, Sydney Urschel, Xiaoli Dong, Allyson L. Brady, Greg F. Slater and Marc Strous

    Biotechnology for Biofuels 2017 10:84

    Published on: 29 March 2017

  20. Research

    Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover

    Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has sh...

    Christa Gunawan, Saisi Xue, Sivakumar Pattathil, Leonardo da Costa Sousa, Bruce E. Dale and Venkatesh Balan

    Biotechnology for Biofuels 2017 10:82

    Published on: 29 March 2017

  21. Research

    Upgrading syngas fermentation effluent using Clostridium kluyveri in a continuous fermentation

    The product of current syngas fermentation systems is an ethanol/acetic acid mixture and the goal is to maximize ethanol recovery. However, ethanol currently has a relatively low market value and its separatio...

    Sylvia Gildemyn, Bastian Molitor, Joseph G. Usack, Mytien Nguyen, Korneel Rabaey and Largus T. Angenent

    Biotechnology for Biofuels 2017 10:83

    Published on: 29 March 2017

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