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  1. Research

    Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution

    Microalgae are highly efficient cellular factories that capture CO2 and are also alternative feedstock for biofuel production. Carbohydrates, proteins, and lipids are major biochemical components in microalgae. C...

    Dujia Cheng, Dengjin Li, Yizhong Yuan, Lin Zhou, Xuyang Li, Tong Wu, Liang Wang, Quanyu Zhao, Wei Wei and Yuhan Sun

    Biotechnology for Biofuels 2017 10:75

    Published on: 24 March 2017

  2. Research

    Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus

    Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as ‘not classified glycosyltransferases (GTnc)’ due to amino acid similari...

    Yongil Yang, Chang Geun Yoo, Hao-Bo Guo, William Rottmann, Kimberly A. Winkeler, Cassandra M. Collins, Lee E. Gunter, Sara S. Jawdy, Xiaohan Yang, Hong Guo, Yunqiao Pu, Arthur J. Ragauskas, Gerald A. Tuskan and Jin-Gui Chen

    Biotechnology for Biofuels 2017 10:74

    Published on: 23 March 2017

  3. Research

    Ternary ionic liquid–water pretreatment systems of an agave bagasse and municipal solid waste blend

    Pretreatment is necessary to reduce biomass recalcitrance and enhance the efficiency of enzymatic saccharification for biofuel production. Ionic liquid (IL) pretreatment has gained a significant interest as a ...

    Jose A. Perez-Pimienta, Noppadon Sathitsuksanoh, Vicki S. Thompson, Kim Tran, Teresa Ponce-Noyola, Vitalie Stavila, Seema Singh and Blake A. Simmons

    Biotechnology for Biofuels 2017 10:72

    Published on: 21 March 2017

  4. Research

    Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform

    The feasibility of heterotrophic–phototrophic symbioses was tested via pairing of yeast strains Cryptococcus curvatus, Rhodotorula glutinis, or Saccharomyces cerevisiae with a sucrose-secreting cyanobacterium Syn...

    Tingting Li, Chien-Ting Li, Kirk Butler, Stephanie G. Hays, Michael T. Guarnieri, George A. Oyler and Michael J. Betenbaugh

    Biotechnology for Biofuels 2017 10:55

    Published on: 21 March 2017

  5. Research

    Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

    Cellulose-degrading thermophilic anaerobic bacterium as a suitable host for consolidated bioprocessing (CBP) has been proposed as an economically suited platform for the production of second-generation biofuel...

    Nisha Singh, Anshu S. Mathur, Deepak K. Tuli, Ravi. P. Gupta, Colin J. Barrow and Munish Puri

    Biotechnology for Biofuels 2017 10:73

    Published on: 21 March 2017

  6. Research

    Metabolic engineering of Saccharomyces cerevisiae to produce a reduced viscosity oil from lignocellulose

    Acetyl-triacylglycerols (acetyl-TAGs) are unusual triacylglycerol (TAG) molecules that contain an sn-3 acetate group. Compared to typical triacylglycerol molecules (here referred to as long chain TAGs; lcTAGs), a...

    Tam N. T. Tran, Rebecca J. Breuer, Ragothaman Avanasi Narasimhan, Lucas S. Parreiras, Yaoping Zhang, Trey K. Sato and Timothy P. Durrett

    Biotechnology for Biofuels 2017 10:69

    Published on: 20 March 2017

  7. Research

    Comparative insights into the saccharification potentials of a relatively unexplored but robust Penicillium funiculosum glycoside hydrolase 7 cellobiohydrolase

    GH7 cellobiohydrolases (CBH1) are vital for the breakdown of cellulose. We had previously observed the enzyme as the most dominant protein in the active cellulose-hydrolyzing secretome of the hypercellulolytic...

    Funso Emmanuel Ogunmolu, Navya Bhatt Kammachi Jagadeesha, Rakesh Kumar, Pawan Kumar, Dinesh Gupta and Syed Shams Yazdani

    Biotechnology for Biofuels 2017 10:71

    Published on: 20 March 2017

  8. Research

    Vapor-fed bio-hybrid fuel cell

    Concentration and purification of ethanol and other biofuels from fermentations are energy-intensive processes, with amplified costs at smaller scales. To circumvent the need for these processes, and to potent...

    Marcus S. Benyamin, Justin P. Jahnke and David M. Mackie

    Biotechnology for Biofuels 2017 10:68

    Published on: 17 March 2017

  9. Research

    Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance

    Resistance to deconstruction is a major limitation to the use of lignocellulosic biomass as a substrate for the production of fuels and chemicals. Consolidated bioprocessing (CBP), the use of microbes for the ...

    Sun-Ki Kim, Joseph Groom, Daehwan Chung, James Elkins and Janet Westpheling

    Biotechnology for Biofuels 2017 10:66

    Published on: 15 March 2017

  10. Research

    Techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons

    Ethanol production from lignocellulosic feedstocks (also known as 2nd generation or 2G ethanol process) presents a great potential for reducing both ethanol production costs and climate change impacts since ag...

    Tassia L. Junqueira, Mateus F. Chagas, Vera L. R. Gouveia, Mylene C. A. F. Rezende, Marcos D. B. Watanabe, Charles D. F. Jesus, Otavio Cavalett, Artur Y. Milanez and Antonio Bonomi

    Biotechnology for Biofuels 2017 10:50

    Published on: 14 March 2017

  11. Research

    Life-cycle analysis of greenhouse gas emissions from renewable jet fuel production

    The introduction of renewable jet fuel (RJF) is considered an important emission mitigation measure for the aviation industry. This study compares the well-to-wake (WtWa) greenhouse gas (GHG) emission performa...

    Sierk de Jong, Kay Antonissen, Ric Hoefnagels, Laura Lonza, Michael Wang, André Faaij and Martin Junginger

    Biotechnology for Biofuels 2017 10:64

    Published on: 14 March 2017

  12. Research

    The Podospora anserina lytic polysaccharide monooxygenase PaLPMO9H catalyzes oxidative cleavage of diverse plant cell wall matrix glycans

    The enzymatic conversion of plant biomass has been recently revolutionized by the discovery of lytic polysaccharide monooxygenases (LPMO) that catalyze oxidative cleavage of polysaccharides. These powerful enz...

    Mathieu Fanuel, Sona Garajova, David Ropartz, Nicholas McGregor, Harry Brumer, Hélène Rogniaux and Jean-Guy Berrin

    Biotechnology for Biofuels 2017 10:63

    Published on: 11 March 2017

  13. Research

    Novel Acinetobacter parvus HANDI 309 microbial biomass for the production of N-acetyl-β-d-glucosamine (GlcNAc) using swollen chitin substrate in submerged fermentation

    N-acetyl-β-d-glucosamine (GlcNAc)6 is extensively used as an important bio-agent and a functional food additive. The traditional chemical process for GlcNAc production has some problem...

    Tae Il Kim, Kwang Seok Ki, Dong Hyun Lim, Mayakrishnan Vijayakumar, Seong Min Park, Sun Ho Choi, Ki Young Kim, Seok Ki Im and Beom Young Park

    Biotechnology for Biofuels 2017 10:59

    Published on: 9 March 2017

  14. Research

    Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

    Microalgae accumulate a considerable amount of lipids and carbohydrate under nutrient-deficient conditions, which makes them one of the promising sustainable resources for biofuel production. In the present st...

    Kaumeel Chokshi, Imran Pancha, Arup Ghosh and Sandhya Mishra

    Biotechnology for Biofuels 2017 10:60

    Published on: 9 March 2017

  15. Research

    A mild thermomechanical process for the enzymatic conversion of radiata pine into fermentable sugars and lignin

    Conversion of softwoods into sustainable fuels and chemicals is important for parts of the world where softwoods are the dominant forest species. While they have high theoretical sugar yields, softwoods are am...

    Ian D. Suckling, Michael W. Jack, John A. Lloyd, Karl D. Murton, Roger H. Newman, Trevor R. Stuthridge, Kirk M. Torr and Alankar A. Vaidya

    Biotechnology for Biofuels 2017 10:61

    Published on: 9 March 2017

  16. Research

    Microbial solvent formation revisited by comparative genome analysis

    Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents ar...

    Anja Poehlein, José David Montoya Solano, Stefanie K. Flitsch, Preben Krabben, Klaus Winzer, Sharon J. Reid, David T. Jones, Edward Green, Nigel P. Minton, Rolf Daniel and Peter Dürre

    Biotechnology for Biofuels 2017 10:58

    Published on: 9 March 2017

  17. Research

    Systematic and functional identification of small non-coding RNAs associated with exogenous biofuel stress in cyanobacterium Synechocystis sp. PCC 6803

    The unicellular model cyanobacterium Synechocystis sp. PCC 6803 is considered a promising microbial chassis for biofuel production. However, its low tolerance to biofuel toxicity limits its potential application....

    Guangsheng Pei, Tao Sun, Shuo Chen, Lei Chen and Weiwen Zhang

    Biotechnology for Biofuels 2017 10:57

    Published on: 7 March 2017

  18. Research

    Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002

    Future sustainable energy production can be achieved using mass cultures of photoautotrophic microorganisms, which are engineered to synthesize valuable products directly from CO2 and sunlight. As cyanobacteria c...

    Joachim Kopka, Stefanie Schmidt, Frederik Dethloff, Nadin Pade, Susanne Berendt, Marco Schottkowski, Nico Martin, Ulf Dühring, Ekaterina Kuchmina, Heike Enke, Dan Kramer, Annegret Wilde, Martin Hagemann and Alexandra Friedrich

    Biotechnology for Biofuels 2017 10:56

    Published on: 6 March 2017

  19. Research

    Production of high concentrated cellulosic ethanol by acetone/water oxidized pretreated beech wood

    Lignocellulosic biomass is an abundant and inexpensive resource for biofuel production. Alongside its biotechnological conversion, pretreatment is essential to enable efficient enzymatic hydrolysis by making c...

    Constantinos Katsimpouras, Konstantinos G. Kalogiannis, Aggeliki Kalogianni, Angelos A. Lappas and Evangelos Topakas

    Biotechnology for Biofuels 2017 10:54

    Published on: 28 February 2017

  20. Research

    Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production

    The high crystallinity of cellulosic biomass myofibrils as well as the complexity of their intermolecular structure is a significant impediment for biofuel production. Cloning of celB-, celC-encoded cellulases (c...

    Eman Ibrahim, Kim D. Jones, Keith E. Taylor, Ebtesam N. Hosseney, Patrick L. Mills and Jean M. Escudero

    Biotechnology for Biofuels 2017 10:52

    Published on: 27 February 2017

  21. Research

    Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in Saccharomyces cerevisiae

    Cellulase expression via extracellular secretion or surface display in Saccharomyces cerevisiae is one of the most frequently used strategies for a consolidated bioprocess (CBP) of cellulosic ethanol production. ...

    Hongting Tang, Meihui Song, Yao He, Jiajing Wang, Shenghuan Wang, Yu Shen, Jin Hou and Xiaoming Bao

    Biotechnology for Biofuels 2017 10:53

    Published on: 27 February 2017

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