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Table 3 Purification of Pichia stipitis XR from strain TMB3260 grown in defined mineral medium supplemented with glucose.

From: Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)

Method

Amount Protein (mg)

Total Activity (U)

Specific Activity (U/mg)

Purification fold

X-Press

725

478.5

0.66

1

Red-Sepharose

9

32.5

3.61

5.5

Gel Filtration

2.8

22.5

8.04

12.2