Skip to main content

Table 3 Purification of Pichia stipitis XR from strain TMB3260 grown in defined mineral medium supplemented with glucose.

From: Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)

Method Amount Protein (mg) Total Activity (U) Specific Activity (U/mg) Purification fold
X-Press 725 478.5 0.66 1
Red-Sepharose 9 32.5 3.61 5.5
Gel Filtration 2.8 22.5 8.04 12.2