Plasmid | Features | Reference |
---|---|---|
prDNAAraA | pBluescript, NTS2::pHXT7 tr -AraA(B. subtilis)-tCYC1 | [27] |
prDNAAraD | pBluescript, NTS2::pHXT7 tr -AraD (E. coli)-tCYC1 | [27] |
pY7 | XYL1 (P. stipitis), XYL2 (P. stipitis), URA3 | [28] |
YEplacHXT-XIp | pHXT7 tr - XI (Pyromyces sp.)-tCYC1, URA3 | [29] |
YIpAraB | KanMX, pHXT7 tr -AraB (E. coli)-tCYC1, TRP1 | [27] |
YIplac128 | LEU2 | [53] |
Strain | Genotype | Reference |
TMB3042 | CEN.PK 2-1C, Δgre3, his3:: p PGK1-XKS1- t PGK1, TAL1:: p PGK1-TAL1- t PGK1, TKL1:: p PGK1-TKL1- t PGK1, RKI1:: p PGK1-RKI1- t PGK1, RPE1:: p PGK1-RPE1- t PGK1, leu2, trp1, ura3 | [31] |
TMB3070 | TMB3042, YIpAraB | This work |
TMB3073 | TMB3070, pHXT7 tr -AraA(B. subtilis)-tCYC1, NTS2::pHXT7 tr -AraD (E. coli)-tCYC1 | This work |
TMB3074 | TMB3073, YIplac128 | This work |
TMB3075 (xylose reductase/xylitol dehydrogenase strain) | TMB3074, pY7 | This work |
TMB3076 (xylose isomerase strain) | TMB3074, YEplacHXT-XIp | This work |