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Table 1 Plasmids and strains used in this study

From: Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains

Plasmid

Features

Reference

prDNAAraA

pBluescript, NTS2::pHXT7 tr -AraA(B. subtilis)-tCYC1

[27]

prDNAAraD

pBluescript, NTS2::pHXT7 tr -AraD (E. coli)-tCYC1

[27]

pY7

XYL1 (P. stipitis), XYL2 (P. stipitis), URA3

[28]

YEplacHXT-XIp

pHXT7 tr - XI (Pyromyces sp.)-tCYC1, URA3

[29]

YIpAraB

KanMX, pHXT7 tr -AraB (E. coli)-tCYC1, TRP1

[27]

YIplac128

LEU2

[53]

Strain

Genotype

Reference

TMB3042

CEN.PK 2-1C, Δgre3, his3:: p PGK1-XKS1- t PGK1, TAL1:: p PGK1-TAL1- t PGK1, TKL1:: p PGK1-TKL1- t PGK1, RKI1:: p PGK1-RKI1- t PGK1, RPE1:: p PGK1-RPE1- t PGK1, leu2, trp1, ura3

[31]

TMB3070

TMB3042, YIpAraB

This work

TMB3073

TMB3070, pHXT7 tr -AraA(B. subtilis)-tCYC1, NTS2::pHXT7 tr -AraD (E. coli)-tCYC1

This work

TMB3074

TMB3073, YIplac128

This work

TMB3075 (xylose reductase/xylitol dehydrogenase strain)

TMB3074, pY7

This work

TMB3076 (xylose isomerase strain)

TMB3074, YEplacHXT-XIp

This work