Figure 4From: Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assayResponse to the addition of chloramphenicol. E. coli strains crp*-gfp and crp*-gfp- grown in modified 1 × M9 minimal media, containing 20% d-glucose, with or without chloramphenicol. Chloramphenicol added at 13 h. (a) Each time point for hours 0 to 12 is the average absorbance of six replications. Each time point for hours 13 to 23 is the average absorbance of three replications. (b) Each time point for hours 0 to 12 is the average fluorescence of six replications of crp*-gfp cultures minus the average fluorescence of six replications of crp*-gfp- cultures. Each time point for hours 13 to 23 is the average fluorescence of three replications of crp*-gfp cultures minus the average fluorescence of three replications of crp*-gfp- cultures. All points are the mean ± s.e.Back to article page