Table 1 Identification of the protein-spots by MALDI-TOF mass spectrometry.
From: Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains
Spot number | Locusa | Protein name | Predb MW/pI | Exptc MW/pI | GPMAW Optimised Score | % sequence coverage |
|---|---|---|---|---|---|---|
1 | ORF_123283 | Arabinofuranosidase (ABFI) | 51.1/6.0 | 53/6.3 | 432 | 38 |
2 | ORF_76210 | Arabinofuranosidase (ABFII) | 34.8/6.4 | 33/6.7 | 176 | 34 |
3 | ORF_55319 | Arabinofuranosidase (ABFIII) | 53.1/5.7 | 55/5.5 | 168 | 16 |
4 | ORF_54219 | Candidate acetyl xylan esterase (AXE) | 21.9/6.2 | 27/6.2 | 222 | 19 |
5 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 63/4.5 | 80 | 7 |
6 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 63/4.4 | 64 | 8,8 |
7 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 63/4.6 | 86 | 9 |
8 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 57/4.7 | 107 | 13 |
9 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 56/5.2 | 210 | 24 |
10 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 58/5.0 | 153 | 11 |
11 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 59/4.8 | 207 | 18 |
12 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 58/6.0 | 165 | 17 |
13 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 55/5.6 | 125 | 11 |
14 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 55/5.4 | 77 | 4 |
15 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 42/4.7 | 157 | 20 |
16 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 38/4.9 | 279 | 17 |
17 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 38/5.0 | 307 | 20 |
18 | ORF_72567 | Cellobiohydrolase II (Cel6A) | 49.6/5.1 | 30/5.1 | 279 | 17 |
19 | ORF_122081 | Endoglucanase I (Cel7B) | 48.2/4.7 | 55/4.6 | 57 | 9 |
20 | ORF_120312 | Endoglucanase II (Cel5A) | 44.1/5.0 | 43/4.8 | 160 | 31 |
21 | ORF_120312 | Endoglucanase II (Cel5A) | 44.1/5.0 | 48/4.6 | 64 | 12 |
22 | ORF_123232 | Endoglucanase III (Cel12A) | 25.1/6.7 | 25/5.7 | 185 | 19 |
23 | ORF_123232 | Endoglucanase III (Cel12A) | 25.1/6.7 | 26/57 | 185 | 19 |
24 | ORF_49081 | Xyloglucanase (Cel74A) | 87.1/5.4 | 96/5.4 | 201 | 16 |
25 | ORF_49081 | Xyloglucanase (Cel74A) | 36.2/8.7 | 96/5.3 | 520 | 26 |
26 | ORF_49081 | Xyloglucanase (Cel74A) | 36.2/8.7 | 43/5.2 | 406 | 21 |
27 | ORF_49081 | Xyloglucanase (Cel74a) | 36.2/8.7 | 35/6.0 | 354 | 12 |
28 | ORF_27554 | Candidate Endoglucanase (EGL) | 36.2/8.7 | 36/5.5 | 87 | 15 |
29 | ORF_121127 | Xylosidase I (BXLI) | 87.2/5.5 | 97/5.6 | 208 | 12 |
30 | ORF_121127 | Xylosidase I (BXLI) | 87.2/5.5 | 97/5.7 | 203 | 15 |
31 | ORF_74223 | Xylanase I (XYNI) | 24.6/5.0 | 21/4.6 | 85 | 16 |
32 | ORF_123818 | Xylanase II (XYNII) | 24.1/7.9 | 21/6.6 | 212 | 27 |
33 | ORF_111849 | Xylanase IV (XYNIV) | 52.8/5.7 | 55/5.6 | 125 | 13 |
34 | ORF_56996 | Mannanase I (MANI) | 40.2/5.1 | 53/5.1 | 147 | 17 |
35 | ORF_76672 | β-Glucosidase (BGLI) | 78.4/6.4 | 81/6.7 | 440 | 32 |
36 | ORF_73897 | Trypsin-like protease (TRY) | 26.4/5.8 | 29/6.0 | 92 | 28 |