Table 2 Identification of the protein-spots by Nano-LC MSMS mass spectrometry.
From: Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains
Spot number | Locusa | Protein name | Predb MW/pI | Exptc MW/pI | Number of peptides matched | Global Score | % sequence coverage |
|---|---|---|---|---|---|---|---|
37 | ORF_123992 | Swollenin (SWO) | 51.5/4.8 | 80/4.6 | 8 | 296 | 20.3 |
38 | ORF_123992 | Swollenin (SWO) | 51.5/4.8 | 80/4.7 | 6 | 252 | 20.3 |
19 | ORF_1220081 | Endoglucanase I (Cel7B) | 48.2/4.7 | 55/4.6 | 13 | 477 | 31.6 |
39 | ORF_120312 | Endoglucanase II (Cel5A) | 44.1/5.0 | 50/4.5 | 9 | 181 | 32.3 |
21 | ORF_120312 | Endoglucanase II (Cel5A) | 44.1/5.0 | 48/4.6 | 13 | 451 | 39.5 |
40 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 55/4.4 | 4 | 114 | 10.1 |
41 | ORF_123989 | Cellobiohydrolase I (Cel7A) | 54.1/4.6 | 47/4.4 | 2 | 65 | 3.1 |
42 | ORF_73638 | Cellulose binding protein (CIPI) | 32.9/4.9 | 43/4.6 | 5 | 171 | 11.1 |
43 | ORF_73638 | Cellulose binding protein (CIPI) | 32.9/4.9 | 47/4.5 | 9 | 250 | 39.9 |
44 | ORF_123940 | Cellulose binding protein (CIPII) | 48.3/7.0 | 57/6.6 | 7 | 281 | 15.2 |
45 | ORF_73643 | Endoglucanase IV (Cel61A) | 35.5/5.3 | 47/5.2 | 1 | 36d | 3.8 |