Figure 1From: Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptusEffect of substrate concentration on the enzymatic digestibility of eucalyptus treated with ball milling. To prepare a large quantity of the sample, ball-milling (BM) treatment (240-minute milling) was performed using pulverisette 5 as described in materials and methods. Various amounts of substrates were mixed and hydrolyzed for 72 hours with the same volume of enzyme cocktail (4 FPU/g substrate). Yields of hydrolyzed glucose and xylose are expressed as a percentage of the theoretical amount based on the glucan and xylan contents, respectively, in the dry weight of untreated biomass.Back to article page