Specific enzyme activities for ethanologenic strain CCE14 normalized to parental strain E. coli C during exponential (A) and stationary phases (B) of culture. All measurements were performed in triplicate. The results presented are three independent experiments. A t-student test with a p value of ≤ 0.05 was applied to each set of normalized values in order to determinate statistical differences enzyme activity levels. Glucose – PEP phosphostransferase (PTS), glucose-6-phosphate dehydrogenase (ZWF), glucose phosphate isomerase (PGI), 6-phosphofructosekinase (PFK), fructose-1,6-bisphosphatase (FDP), fructose bisphospate aldolase (FDP aldolase), triose phosphate isomerase (TPI), glyceldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK), phosphoglycerate mutase (PGM), pyruvate kynase (PYK), pyruvate-formate lyase (PFL), lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH
) and pyruvate decarboxylase (PDCZm).