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Table 1 Steam pretreatment conditions

From: Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house

Substrate Spruce Wheat straw Bagasse
Size (mm) 2–10 2–10 Not determined
Prior to pretreatment Impregnated with 2.5% SO2 (w/w moisture) Soaked in 0.2% H2SO4 solution (20 g solution/g dry straw) Impregnated with 2% SO2 (w/w moisture)
Pretreatment temperature (°C) 210 190 200
Pretreatment time (min) 5 10 5