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Table 3 Activities of the enzymes used in hydrolysis

From: Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house

Enzyme

Enzyme activity

 

FPA (FPU/g)

β-glucosidase (IU/g)

Xylanase (IU/g)

β-xylosidase (IU/g)

Endoglucanase (IU/g)

Mannanase (IU/g)

β-mannosidase (IU/g)

Celluclast 1.5 L

56.2

30.8

750

63.6

13087

122

~0.08

Novozym 188

n.d.

502

34.7

6.7

296

17.9

5.4

Multifect xylanase

0.15

26.6

22969

26.5

454

n.a.

n.a.

Pulpzyme HC

17.9

12.9

1343

118.2

6961

n.a.

n.a.

F-1663/S

0.36

7.6

5.0

<0.01

71.6

1.1

~0.02

F-1663/WS

0.36

5.2

39.5

~0.05

72.7

0.98

~0.02

F-1663/B

0.37

4.8

40.1

~0.03

69.6

0.88

~0.02

  1. FPA: filter paper activity; n.d.: not detectable.; n.a.: not analyzed.
  2. F-1663/S, WS, B: the crude enzyme supernatants of Trichoderma atroviride TUB F-1663 produced on steam-pretreated spruce, wheat straw and sugarcane bagasse.
  3. The activities presented are the mean values of at least two separate measurements. The standard deviations are <10%, and mainly 1–4% for FPA, <10% for β-glucosidase, 1–5% for xylanase and β-xylosidase, mostly around 10% for endoglucanase, and 5–10% for mannanase and β-mannosidase measurement.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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