Figure 4

The fermentation of molasses with watermelon juice used as both diluent and supplemental feedstock. The 2007 Hinton, OK, USA watermelon crop was the source of the juice. Closed squares = sugar concentration of molasses diluted with watermelon juice minus chromoplasts. The nitrogen content for this fermentation was 385 mg N/L (6.1 μmol amino N/ml) from watermelon juice and 2066 mg N/L (9.2 μmol amino N/ml) from the molasses. Open squares = ethanol concentration produced from molasses diluted with watermelon juice minus chromoplasts; closed circles = sugar concentration of molasses diluted with chromoplast- and amino acid-free watermelon juice. The nitrogen content for this fermentation was 46 mg N/L (2.7 μmol amino N/ml) from the GoFerm™ and Fermaid K™ and 2066 mg N/L (9.2 μmol amino N/ml) from the molasses. Open circles = ethanol concentration produced from molasses diluted with chromoplast- and amino acid-free watermelon juice. Closed triangles = sugar concentration of molasses diluted with chromoplast- and amino acid-free watermelon juice that was nitrogen-supplemented with 500 mg N/L (22.5 μmol/ml amino N) from yeast extract (5.5 g/L), DAP (1.5 g/L), Go-Ferm™ (0.625 g/L), and Fermaid K™ (0.25 g/L) in addition to the nitrogen from the molasses. Open triangles = ethanol concentration produced from molasses diluted with chromoplast- and amino acid-free watermelon juice that was nitrogen-supplemented with 500 mg N/L in addition to that from the molasses. Other conditions for the fermentations are described in the text.