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Table 1 Cellulase Assays

From: Measurement of saccharifying cellulase

Enzyme Substrate Product measured
Cellobiase   
   β Glucosidase Cellobiose Glucose
  Cellodextrins  
  Salicin Salingenin
  p-Nitro β glucosidase p Nitrophenol
Endo β 1, 4 glucanase   
   Cx Carboxymethyl cellulose Loss in viscosity
   CMC'ase Amorphous cellulose Reducing sugar
  Walseth  
  Sweco  
  Cellodextrins  
Exo β 1, 4 Glucanase   
   A Glucocellulase Amorphous cellulose Glucose (A)
  Walseth  
   B Cellobiohydrolase Crystalline cellulose Cellobiose (B)
   CBH Avicel  
   C1 Cellodextrins  
Cellulase   
   C1 + Cx Crystalline cellulose Loss in weight
   Avicellase    Avicel Reducing sugar
   Hydrocellulase    Hydrocellulose Reduction in optical density (OD)
   FP'ase    Filter paper  
  Cellodextrins  
  Cotton  
Miscellaneous   
   Swelling factor Cotton Uptake of alkali
   Filter paper cellulase Filter paper Maceration [1]
  Thread Breaking strength
  Dyed cellulose Release of dye
  1. CMC = carboxymethyl cellulose; FP = filter paper.