|
Reference
|
Enzyme, ml
|
CMC, ml
|
CMC, % in assay
|
Time, min
|
1 Cx units/ml Glucose mg/ml
|
1 Cx units/ml Glucose total mg
|
International units
Cx units
|
|---|
|
Ab [6]
|
1.0
|
9.0
|
0.5
|
60
|
0.4
|
4.0
|
0.37
|
|
Bc [7]
|
0.5
|
4.5
|
0.5
|
60
|
0.4
|
2.0
|
0.37
|
|
Cd [8]
|
0.5
|
0.5
|
0.5
|
30
|
0.5
|
0.5
|
0.185
|
- aSubstrate = carboxymethyl cellulose (CMC), degree of substitution 0.5. Temperature = 50 C. Product = reducing sugar as glucose. Buffer = 0.05 M citrate pH 4.8. Calculations: 1 IU = 1 μmol of glucose/min (0.18 mg/min). 1 mg glucose/0.18 × 60 = 0.0925 units (1 h) or 1 mg glucose/0.18 × 30 = 0.185 units (0.5 h).
- b4.0 mg glucose × 0.0925/1.0 ml enzyme = 0.37 IU/Cx unit.
- c2.0 mg glucose × 0.0925/0.5 ml enzyme = 0.37 IU/Cx unit.
- d0.5 mg glucose × 0.185/0.5 ml enzyme = 0.185 IU/Cx unit.
