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Table 2 Endo β Glucanase (Cx) Assaya

From: Measurement of saccharifying cellulase

Reference Enzyme, ml CMC, ml CMC, % in assay Time, min 1 Cx units/ml Glucose mg/ml 1 Cx units/ml Glucose total mg International units Cx units
Ab [6] 1.0 9.0 0.5 60 0.4 4.0 0.37
Bc [7] 0.5 4.5 0.5 60 0.4 2.0 0.37
Cd [8] 0.5 0.5 0.5 30 0.5 0.5 0.185
  1. aSubstrate = carboxymethyl cellulose (CMC), degree of substitution 0.5. Temperature = 50 C. Product = reducing sugar as glucose. Buffer = 0.05 M citrate pH 4.8. Calculations: 1 IU = 1 μmol of glucose/min (0.18 mg/min). 1 mg glucose/0.18 × 60 = 0.0925 units (1 h) or 1 mg glucose/0.18 × 30 = 0.185 units (0.5 h).
  2. b4.0 mg glucose × 0.0925/1.0 ml enzyme = 0.37 IU/Cx unit.
  3. c2.0 mg glucose × 0.0925/0.5 ml enzyme = 0.37 IU/Cx unit.
  4. d0.5 mg glucose × 0.185/0.5 ml enzyme = 0.185 IU/Cx unit.