Measurement of saccharifying cellulase

Table 2 Endo β Glucanase (C_x) Assay^a

Reference	Enzyme, ml	CMC, ml	CMC, % in assay	Time, min	1 C_x units/ml Glucose mg/ml	1 C_x units/ml Glucose total mg	International units C_x units
A^b [6]	1.0	9.0	0.5	60	0.4	4.0	0.37
B^c [7]	0.5	4.5	0.5	60	0.4	2.0	0.37
C^d [8]	0.5	0.5	0.5	30	0.5	0.5	0.185

^aSubstrate = carboxymethyl cellulose (CMC), degree of substitution 0.5. Temperature = 50 C. Product = reducing sugar as glucose. Buffer = 0.05 M citrate pH 4.8. Calculations: 1 IU = 1 μmol of glucose/min (0.18 mg/min). 1 mg glucose/0.18 × 60 = 0.0925 units (1 h) or 1 mg glucose/0.18 × 30 = 0.185 units (0.5 h).
^b4.0 mg glucose × 0.0925/1.0 ml enzyme = 0.37 IU/C_x unit.
^c2.0 mg glucose × 0.0925/0.5 ml enzyme = 0.37 IU/C_x unit.
^d0.5 mg glucose × 0.185/0.5 ml enzyme = 0.185 IU/C_x unit.

ISSN: 2731-3654