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Table 3 Filter Paper (FP) Activity and Unitsa

From: Measurement of saccharifying cellulase

International units/ml Filter Paper Activity ml enzyme for 2.0 mg glucose
  1.0 ml 0.5 ml 0.2 ml 0.1 ml  
0.05 0.54 0.27 - - -
0.10 1.08 0.54 - - -
0.20 2.10 1.08 0.40 - 0.925
0.30 2.50 1.62 0.80 - 0.615
0.40 2.80 2.10 1.10 0.40 0.463
0.50 3.00 2.30 1.40 0.80 0.370
0.75 3.90 2.80 1.75 1.10 0.248
1.00 4.60 3.00 2.10 1.40 0.185
1.50 - 3.90 2.65 1.75 0.124
2.00 - 4.60 3.20 2.10 0.093
  1. aSubstrate: 50 mg Whatman No. 1 filter paper (6 cm2). Buffer: 1.0 ml, 0.05 M citrate pH 4.8. Enzyme: 1 ml assay, volume indicated + buffer to 1.0 ml FP activity × 0.0925 = IU/ml; 0.5 ml assay volume indicated + buffer to 0.5 ml FP activity × 0.185 IU/ml or if FP activity is greater than 2.0 IU/ml = 0.185/ml enzyme for 2.0 mg glucose. (2.0 mg glucose = 4% hydrolysis of 50 mg; the unit based on 0.5 mg of glucose from 1 ml of enzyme [8] is equal to 0.0463 IU.)