|
International units/ml
|
Filter
|
Paper
|
Activity
|
ml enzyme for 2.0 mg glucose
|
|---|
| |
1.0 ml
|
0.5 ml
|
0.2 ml
|
0.1 ml
| |
|---|
|
0.05
|
0.54
|
0.27
|
-
|
-
|
-
|
|
0.10
|
1.08
|
0.54
|
-
|
-
|
-
|
|
0.20
|
2.10
|
1.08
|
0.40
|
-
|
0.925
|
|
0.30
|
2.50
|
1.62
|
0.80
|
-
|
0.615
|
|
0.40
|
2.80
|
2.10
|
1.10
|
0.40
|
0.463
|
|
0.50
|
3.00
|
2.30
|
1.40
|
0.80
|
0.370
|
|
0.75
|
3.90
|
2.80
|
1.75
|
1.10
|
0.248
|
|
1.00
|
4.60
|
3.00
|
2.10
|
1.40
|
0.185
|
|
1.50
|
-
|
3.90
|
2.65
|
1.75
|
0.124
|
|
2.00
|
-
|
4.60
|
3.20
|
2.10
|
0.093
|
- aSubstrate: 50 mg Whatman No. 1 filter paper (6 cm2). Buffer: 1.0 ml, 0.05 M citrate pH 4.8. Enzyme: 1 ml assay, volume indicated + buffer to 1.0 ml FP activity × 0.0925 = IU/ml; 0.5 ml assay volume indicated + buffer to 0.5 ml FP activity × 0.185 IU/ml or if FP activity is greater than 2.0 IU/ml = 0.185/ml enzyme for 2.0 mg glucose. (2.0 mg glucose = 4% hydrolysis of 50 mg; the unit based on 0.5 mg of glucose from 1 ml of enzyme [8] is equal to 0.0463 IU.)
