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Table 4 Effect of Conditions on Filter Paper Assaya

From: Measurement of saccharifying cellulase

Filer paper mg 25 50 50 100
Enzyme ml 0.5 0.5 1.0 0.5
Enzyme protein mg 0.7 0.7 1.4 0.7
Activity mg glucose 30 min 2.29 2.43 3.52 5.18
  60 min 3.30 3.68 4.40 5.53
  120 min 4.60 5.30 6.75 7.16
Activity per h 30 min 4.58 4.86 7.04 10.36
  60 min 3.30 3.68 4.40 5.53
  120 min 2.30 2.65 3.38 3.58
Units/ml 30 min 0.93 1.23 1.19 4.35
  60 min 0.76 1.23 1.23 3.52
  120 min 0.80 1.42 1.32 3.08
Units/mg protein 30 min 0.66 0.88 0.85 3.11
  60 min 0.54 0.88 0.88 2.51
  120 min 0.57 1.01 0.94 2.61
  1. a0.5 or 1.0 ml of enzyme (culture filtrate of QM9414 grown on cellulose) + 1 ml of pH 4.8 buffer + Strip of Whatman No. 1 filter paper. Units = μmol of glucose per min based on the dilution to give 2.0 mg glucose.