Table 1 Plasmids and S. cerevisiae strains used in this study.
Plasmids and Strains | Relevant genotype | Reference |
|---|---|---|
pY7 | ADH1p-XYL1-ADH1t, PGK1p-XYL2-PGK1t, URA3, 2 μ | [31] |
YIplac211 PGK | PGK1p-PGK1t, URA3 | [13] |
YIplac211 PGK XYL1(K270M) | PGK1p-XYL1(K270M)-PGK1t, URA3 | [13] |
YIplac211 PGK XYL1(K270R) | PGK1p-XYL1(K270R)-PGK1t, URA3 | This work |
pUC57-CpXR | XYL1(C. parapsilosis) | This work |
YIplac128 | LEU2 | [32] |
YIplac211 | URA3 | [32] |
YIpOB1 | ADH1p-XYL1-ADH1t, PGK1p-XYL2-PGK1t, LEU2 | This work |
YIpOB2 | ADH1p-XYL1-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
YIpOB3 | ADH1p-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
YIpOB4 | ADH1p-XYL1(K270M)-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
YIpOB5 | ADH1p-XYL1(K270R)-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
YIpOB6 | ADH1p-XYL1(C. parapsilosis)-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
TMB 3265 | CEN.PK 113-11C, MAT a, ura3-52, his3::HIS3 YIpXDH/XK | [30] |
TMB 3200 | TMB 3265, ura3::URA3 YIplac211 PGK XYL1(K270R) | This work |
TMB 3044 | CEN.PK 2-1C, MAT a, ura3-52, Δgre3, his3::HIS3 PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t | [22] |
TMB 3321/Y-PsNative | TMB 3044, ura3::URA3 YIpOB2 | This work |
TMB 3322/Y-PsK270M | TMB 3044, ura3::URA3 YIpOB4 | This work |
TMB 3323/Y-PsK270R | TMB 3044, ura3::URA3 YIpOB5 | This work |
TMB 3324/Y-CpXR | TMB 3044, ura3::URA3 YIpOB6 | This work |