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Table 2 Primers used in this study.

From: Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae

Primer Sequence Restriction Endonuclease
5XYL1s 5'-GCGGATCC TCTAGA ATGCCTT-3' Bam HI
3XYL1s 5'-TTGGATCC TCTAGA TTAGACGAAG-3' Bam HI
5K270R 5'-CATCATTCCAAGG TCCAACACTG-3'  
3K270R 5'-CAGTGTTGGACCT TGGAATGATG-3'  
pY7-XR-for 5'-GCAAGCTT GGCGCGCC GGGATCGAAGAAATGATGG-3' Hind III, Asc I
pY7-XR-rev 5'-CGCGCGCGCTGCAG GTGTGGAAGAACGATTACAAC-3' Pst I
pY7-XDH-for 5'-GCCTGCAG TCTAACTGATCTATCCAAAACTG-3' Pst I
pY7-XDH-rev 5'-CGTGAGCTC CGTACG TAACGAACGCAGAATTTTC-3' Sac I, Bsi WI
  1. Sites for restriction endonucleases are indicated in bold or italic. The codon giving the P. stipitis XR Arg at amino acid position 270 is underlined in primers 5K270R and 3K270R.