Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae

Table 5 Continuous fermentation

Dilution rate (h^-1)	Strain	Specific consumption and production rates (g (g biomass)^-1 h^-1)			Yields (g product (g consumed sugars)^-1)				Carbon balance (%)
		Glucose	Xylose	Ethanol	Ethanol	Xylitol^a	Glycerol	Biomass
0.06	Y-PsNative	-0.64 ± 0.02	-0.19 ± 0.01	0.31 ± 0.00	0.37 ± 0.02	0.30 ± 0.02	0.09 ± 0.00	0.07 ± 0.00	96 ± 2
	Y-PsK270R	-0.52 ± 0.01	-0.22 ± 0.01	0.29 ± 0.01	0.39 ± 0.00	0.12 ± 0.01	0.07 ± 0.01	0.08 ± 0.00	94 ± 1
0.12	Y-PsNative	-1.09 ± 0.03	-0.26 ± 0.02	0.50 ± 0.01	0.37 ± 0.02	0.24 ± 0.04	0.09 ± 0.01	0.09 ± 0.00	95 ± 3
	Y-PsK270R	-1.04 ± 0.06	-0.28 ± 0.01	0.51 ± 0.03	0.39 ± 0.01	0.05 ± 0.02	0.07 ± 0.01	0.09 ± 0.00	93 ± 1

Specific consumption (negative) and production (positive) rates, product yields and carbon balances in continuous fermentation of strains Y-PsNative and Y-PsK270R under anaerobic conditions at dilution rates of 0.06 h^-1 and 0.12 h^-1 with 10 g l^-1 glucose and 10 g l^-1 xylose.
^a (g xylitol (g consumed xylose)^-1)

ISSN: 2731-3654