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Table 3 Optimal enzyme loadings for enzymatic hydrolysis of four harvests of switchgrass.

From: Evaluation of ammonia fibre expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations

  CIR: July CIR: October Alamo: July Alamo: October
Accelerasea 5.0 6.4 5.0 5.0
Novozymea 0 0 0 0
Monomer xylanasea 5.0 3.6 5.0 5.0
Monomer pectinasea 5.0 5.0 5.0 5.0
Predicted sugarsb 523 411 409 557
Actual sugarsc 521 410 410 445
Sugar yield (%) 89.0% 60.9% 64.0% 68.9%
Glucose yieldc 321 223 210 237
Xylose yieldc 200 187 201 208
R2 81.3% 76.5% 81.5% 64.9%
  1. aAll values are in mg enzyme per g dry switchgrass; bmonomeric sugars released (g/kg switchgrass) after 72 h of hydrolysis as predicted by the model; cactual monomeric sugars released (g/kg switchgrass) after 72 h of hydrolysis.
  2. CIR = Cave-in-Rock.