Figure 4

Fermentation using Escherichia coli KO11 on yeast extract peptone (5 g/L yeast extract + 10 g/L peptone) media supplemented with commercial enzymes, 15% solids loading equivalent of ammonia fiber explosion (AFEX)-corn stover wash stream (WS) or a combination of commercial enzymes and the WS. Fermentation was conducted at 37°C, pH 7.0 (adjusted during fermentation) and was initiated at 0.5 OD600 nm.