Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production

Table 1 Seed culture media recipe for the three ethanologenic strains

Strain	Temperature (°C)	Buffer/pH	Antibiotics	Sugars concentration	Nitrogen source
KO11	37	0.1 M MOPS/7.0	50 mg/L Chloramphenicol
AX101	30	0.05 M Phosphate/5.5	30 mg/L Ampicillin	50 g/L for Glucose-only and fermentation 30 g/L +20 g/L for co-fermentation and xylose-only	2.0% w/v corn steep liquor (CSL) for fermentation in CSL fermentation; 5.0 g/L yeast extract + 10.0 g/L peptone for wash stream and AFEX hydrolysate fermentation
424A-(LNH-ST)	30	0.05 M Phosphate/5.5	50 mg/L Ampicillin

Note: yeast extract and peptone was only used for seed culture preparation but was not supplemented to the actual ammonia fiber explosion (AFEX)-corn stover hydrolysate during ethanol fermentation.

ISSN: 2731-3654