Figure 5

Effect of ensilation (0X, 1X or 10X) of corn silage (CS) on enzymatic digestibility (total sugar = glucose + xylose + arabinose) before and after ammonia fiber expansion (AFEX) pretreatment. AFEX conditions employed were as follows: temperature 90°C, moisture loading 60% DWB, ammonia to biomass loading 1:1 w/w, and residence time 5 mins. Enzymatic hydrolysis was carried out at 50°C, 150 rpm, 15 mL reaction volume in presence of Accellerase (3.1 mg/ g of cellulose) and Stargen (3.8 mg/g of starch). The lines above all bars indicate the maximum possible total sugar yield based on theoretical composition.