Figure 6

Glucose and xylose yields during high-solid (6% w/w glucan loading) loading-based enzymatic hydrolysis for corn silage (0X-CS) or whole corn plant (WCP) before and after ammonia fiber expansion (AFEX) treatment. AFEX conditions employed were as follows: temperature 90°C, moisture loading 60%, DWB, ammonia to biomass loading 1:1 w/w, and residence time 5 min. Enzymatic hydrolysis was carried out at 50°C, 72 h, 250 rpm, 500 mL reaction volumes in the presence of Accellerase (10 mg/g of cellulose), Stargen (3.50 mg/g starch) and Multifect Xylanase (7.5 mg/g Xylan). The lines above all bars indicate the maximum possible sugar yield based on theoretical composition.