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Table 1 Description and aerobic growth on pentoses for the Saccharomyces cerevisiae strains used in the study

From: Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering

Strains Relevant genotype/phenotype Abbreviation Maximum specific growth rate (h-1) Maximum OD620nm (average) Strain reference
    Xylose Arabinose Xylose Arabinose  
TMB3400 HIS3::ADH1p- XYL1-ADH1t, PGKp-XYL2-PGKt, pPGK-XKS1-tPGK X 0.129 ± 0.010 0.002 ± 0.000 19.1 0.5 [31]
TMB3061 TMB3400, TRP1:: YlpAraB KanMX,
NTS2::HXT7p-AraA-CYC1t and
NTS2:: HXT7p-AraD-CYC1t, KanMX
X+A 0.016 ± 0.000 0.014 ± 0.000 11.1 5.1 [10]
TMB3063 TMB3061, additional copies of
NTS2::HXT7 p-AraA-CYC1 t
X+A+A 0.027 ± 0.000 0.033 ± 0.000 12.1 5.0 [10]
TMB3130 TMB3061, evolved on xylose and arabinose X+A evolved 0.054 ± 0.003 0.027 ± 0.010 23.3 21.5 This study
  1. Growth parameters were determined under aerobic conditions on yeast nitrogen base (YNB) medium with 50 g L-1 arabinose or 50 g L-1 xylose. Cell pre-cultures for the inoculum were grown on YNB medium with 20 g L-1 glucose.
  2. OD, optical density.