Table 1 Description and aerobic growth on pentoses for the Saccharomyces cerevisiae strains used in the study
Strains | Relevant genotype/phenotype | Abbreviation | Maximum specific growth rate (h-1) | Maximum OD620nm (average) | Strain reference | ||
|---|---|---|---|---|---|---|---|
Xylose | Arabinose | Xylose | Arabinose | ||||
TMB3400 | HIS3::ADH1p- XYL1-ADH1t, PGKp-XYL2-PGKt, pPGK-XKS1-tPGK | X | 0.129 ± 0.010 | 0.002 ± 0.000 | 19.1 | 0.5 | [31] |
TMB3061 | TMB3400, TRP1:: YlpAraB KanMX, NTS2::HXT7p-AraA-CYC1t and NTS2:: HXT7p-AraD-CYC1t, KanMX | X+A | 0.016 ± 0.000 | 0.014 ± 0.000 | 11.1 | 5.1 | [10] |
TMB3063 | TMB3061, additional copies of NTS2::HXT7 p-AraA-CYC1 t | X+A+A | 0.027 ± 0.000 | 0.033 ± 0.000 | 12.1 | 5.0 | [10] |
TMB3130 | TMB3061, evolved on xylose and arabinose | X+A evolved | 0.054 ± 0.003 | 0.027 ± 0.010 | 23.3 | 21.5 | This study |