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Table 2 Enzyme feed strategies for fed-batch simultaneous saccharification and co-fermentation of wheat straw (8%-11% WIS)a

From: Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding

Time of addition

(h)

Feed profile A

(amount enzyme)

Feed profile B

(amount enzyme)

Feed profile C

(amount enzyme)

Feed profile D

(amount enzyme)

Initial addition

2/6

1/5

1/5

1/5

6

1/6

1/10

-

-

12

1/6

1/10

-

-

18

1/6

1/10

-

-

24

1/6

1/10

1/5

1/5 (+ 2 g/L yeast)

32

-

-

1/5

1/5

36

-

1/5

-

-

40

-

-

1/5

1/5

48

-

1/5

1/5

1/5

  1. aIn all feed profiles, the same total enzyme load and the same substrate feed were used. Substrate was added after 6, 12, 18 and 24 h.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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