Adsorption isotherms of the purified cellulases onto α-cellulose. (Black circle) Cellobiohydrolase (CBH) I; (white circle) endoglucanase (EG) I. Predicted Langmuir isotherms, according to Eqn (1), are shown as solid lines and corresponding parameters are listed in Table 2. 10 g/L α-cellulose in 0.1 M sodium acetate buffer at pH 4.8, T = 45°C, V
= 1 mL, n = 1000 rpm, d
= 3 mm, reaction time 40 min.