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Figure 6 | Biotechnology for Biofuels

Figure 6

From: Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics

Figure 6

Adsorption kinetics and activities of the purified cellulases onto α-cellulose at different hydrodynamic conditions. (A) Influence of shaking frequency on kinetic adsorption parameters (including standard deviations), maximum cellulase adsorption at equilibrium (A max ; solid line) and pseudo-first-order adsorption rate constant (k ad ; dotted line), according to Eqn (2): (black square) A max of cellobiohydrolase (CBH) I; (white square) A max of endoglucanase (EG) I, (black triangle) k ad of CBH I; (white triangle) k ad of EG I. The critical shaking frequency (n crit ), calculated according to Eqn (3) [54], is indicated with black arrows; (B) Influence of shaking frequency on the activity of cellulases (dashed line): (black circle) CBH I; (white circle) EG I. Relative values (including standard deviations) are standardized to maximum activities (CBH I: 0.25 U/mg; EG I: 0.64 U/mg); (C) pictures of the liquid phase with immersed α-cellulose particles at different shaking frequencies. The white arrow indicates the start of liquid mixing. Images were obtained with a charged-coupled device camera installed on a shaking platform. 10 g/L α-cellulose in 0.1 M sodium acetate buffer at pH 4.8, T = 45°C, V L = 1 mL, n = 0-1000 rpm, d 0 = 3 mm.

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