Table 1 S. cerevisia e strains and plasmids used in this study
S. cerevisiae strains and corresponding abbreviations | Strain background | Relevant genotype/phenotype | Reference |
|---|---|---|---|
TMB 3320 | TMB 3043 | CEN.PK2-1C, Δgre3 his3::HIS3 PGK1p-XK1-PGK1t, PGK1p-TAL1-PGK1t, PGK1p-RKI1-PGK1t, PGK1p-TKL1-PGK1t, PGK1p-RPE1-PGK1t, TRP1, leu2::LEU2 ADH1p-XYL1-ADH1t PGKp-XYL2-PGKt ura3 | Garcia Sanchez et al., manuscript in preparation |
CEN.PK 113-11C | MAT a his3Δ1 ura 3-52 MAL2-8c SUC2 | [42] | |
TMB 3137 "Control-PPP-XYL" | TMB 3320 | ura3::URA3 YIplac211 | This work |
TMB 3138 "PGM2-PPP-XYL" | TMB 3320 | ura3::URA3 YIplac211 HXT-PGM2 | This work |
TMB 3141 | CEN.PK 113-11C | his3:: HIS3 YIpXR/XDH/XK | This work |
TMB 3139 "Control-XYL" | TMB 3141 | his3:: HIS3 YIpXR/XDH/XK ura3::URA3 YIplac211 | This work |
TMB 3140 "PGM2-XYL" | TMB 3141 | his3:: HIS3 YIpXR/XDH/XK, ura3::URA3 YIplac211 HXT-PGM2 | This work |
TMB 3128 "Control m" | CEN.PK 113-11C | his3::HIS3 YEplacHXT URA3 | [16] |
TMB 3129 "PGM2 m " | CEN.PK 113-11C | his3::HIS3 YEplacHXT7'p-PGM2 URA3 | [16] |
TMB 3135 " Control i" | CEN.PK 113-11C | his3::HIS3 ura3::URA3 | [16] |
TMB 3136 "PGM2 i" | CEN.PK 113-11C | his3::HIS3 ura3::URA3 Ylplac211 HXT-PGM2 | [16] |
Plasmids | |||
YIplac211 | URA3 | [43] | |
YIplac211 HXT-PGM2 | YIplac211, HXT7'p-PGM2-PGKt URA3 | This work | |
YIpXR/XDH/XK | ADHp-XYL1-ADHt, PGKp-XYL2-PGKt, PGKp-XK-PGKt, HIS3, β-lactamase | (Eliasson, Christensson et al. 2000) |