Figure 1From: Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiaeFurfural induces the accumulation of reactive oxygen species (ROS). Exponentially growing yeast cells were treated with no inhibitor, 25 mM furfural, 50 mM furfural or 5 mM hydrogen peroxide (positive control for ROS). (A) Representative images of cells stained with the ROS indicator dye 2'7' DCF-diacetate (left column) and differential interference contrast (DIC) (right column) are shown. Images of cells were at the 8 h time point. (B) Percent of yeast cells that stained positive for ROS by 2'7' DCF-diacetate at 0 h and 8 h. Data represent an average of five experiments with standard error indicated. At each time point at least 100 cells were examined.Back to article page