Figure 6

Furfural causes the actin cytoskeleton to go from predominantly cables to patches. Exponentially growing yeast cells were either untreated or treated with 25 mM or 50 mM furfural. (A) Representative images observed with no inhibitor (left column; actin cables in mother cell), 25 mM furfural (middle column; actin patches in mother cell) or 50 mM furfural (right column; actin patches in mother cell) are shown. Images of cells were taken at the 6 h time point. (B) Percent of cells at each concentration of furfural at 0 h, 6 h and 24 h that contain only actin patches similar to right two images in (A). Data represent an average of two experiments with standard error indicated. At each time point 100 cells were examined.