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Table 1 Commercial enzyme benchmarks for release of Glc and Xyl from 15 pretreatment/feedstock combinations*

From: Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Feedstock

Pretreatment

Glc release (%)

Xyl release (%)

  

No enzyme

Spezyme CP

Accellerase 1000

No enzyme

Spezyme CP

Accellerase 1000

Corn stover

AFEX

1.0 ± 0.0

44.0 ± 0.0

50.6 ± 0.3

1.2 ± 0.0

34.4 ± 0.5

30.0 ± 0.6

 

0.25% NaOH

1.1 ± 0.0

40.7 ± 0.1

54.1 ± 0.3

1.2 ± 1.2

35.4 ± 0.2

22.9 ± 0.3

 

Alk. peroxide

0.4 ± 0.0

58.4 ± 0.1

68.6 ± 0.3

0.0 ± 0.0

49.6 ± 0.6

38.0 ± 0.6

Switchgrass

AFEX

2.1 ± 0.0

22.9 ± 0.1

28.1 ± 0.0

0.0 ± 0.0

22.6 ± 0.7

23.4 ± 0.1

 

0.25% NaOH

0.6 ± 0.1

16.4 ± 0.1

31.1 ± 0.1

0.0 ± 0.0

30.5 ± 0.2

23.2 ± 0.1

 

Alk. peroxide

1.4 ± 0.0

41.6 ± 0.4

50.6 ± 0.1

0.0 ± 0.0

45.1 ± 0.2

34.6 ± 0.3

Miscanthus

AFEX

1.2 ± 0.3

36.2 ± 0.3

35.1 ± 0.0

0.0 ± 0.0

36.9 ± 1.0

36.8 ± 0.5

 

0.25% NaOH

0.0 ± 0.0

13.0 ± 0.0

23.1 ± 0.3

0.7 ± 0.3

27.8 ± 0.2

22.2 ± 0.3

 

Alk. peroxide

0.4 ± 0.1

37.7 ± 0.6

43.8 ± 0.3

0.8 ± 0.3

54.8 ± 2.2

42.3 ± 0.2

DDGS

AFEX

0.8 ± 0.1

32.6 ± 0.4

42.6 ± 0.4

1.1 ± 0.7

5.6 ± 0.2

4.9 ± 1.2

 

0.25% NaOH

1.2 ± 0.1

26.4 ± 0.2

32.0 ± 0.2

0.8 ± 0.1

3.8 ± 0.8

3.5 ± 0.6

 

Alk. peroxide

2.8 ± 0.1

31.5 ± 0.2

38.1 ± 0.3

2.1 ± 0.5

7.3 ± 0.6

6.4 ± 0.8

Poplar

AFEX

2.1 ± 0.3

18.0 ± 0.1

17.6 ± 0.0

0.0 ± 0.0

21.3 ± 0.8

21.5 ± 0.3

 

0.25% NaOH

0.1 ± 0.1

10.3 ± 0.3

17.1 ± 0.4

0.3 ± 0.0

44.4 ± 0.1

32.3 ± 0.5

 

Alk. peroxide

0.8 ± 0.0

17.8 ± 0.2

18.0 ± 0.0

0.1 ± 0.1

43.9 ± 1.4

37.3 ± 0.7

  1. *Glc and Xyl release are expressed as a percentage of total Glc and Xyl in each substrate (Additional file 1, Table S2). Data are expressed as the means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan.