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Table 1 Commercial enzyme benchmarks for release of Glc and Xyl from 15 pretreatment/feedstock combinations*

From: Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Feedstock Pretreatment Glc release (%) Xyl release (%)
   No enzyme Spezyme CP Accellerase 1000 No enzyme Spezyme CP Accellerase 1000
Corn stover AFEX 1.0 ± 0.0 44.0 ± 0.0 50.6 ± 0.3 1.2 ± 0.0 34.4 ± 0.5 30.0 ± 0.6
  0.25% NaOH 1.1 ± 0.0 40.7 ± 0.1 54.1 ± 0.3 1.2 ± 1.2 35.4 ± 0.2 22.9 ± 0.3
  Alk. peroxide 0.4 ± 0.0 58.4 ± 0.1 68.6 ± 0.3 0.0 ± 0.0 49.6 ± 0.6 38.0 ± 0.6
Switchgrass AFEX 2.1 ± 0.0 22.9 ± 0.1 28.1 ± 0.0 0.0 ± 0.0 22.6 ± 0.7 23.4 ± 0.1
  0.25% NaOH 0.6 ± 0.1 16.4 ± 0.1 31.1 ± 0.1 0.0 ± 0.0 30.5 ± 0.2 23.2 ± 0.1
  Alk. peroxide 1.4 ± 0.0 41.6 ± 0.4 50.6 ± 0.1 0.0 ± 0.0 45.1 ± 0.2 34.6 ± 0.3
Miscanthus AFEX 1.2 ± 0.3 36.2 ± 0.3 35.1 ± 0.0 0.0 ± 0.0 36.9 ± 1.0 36.8 ± 0.5
  0.25% NaOH 0.0 ± 0.0 13.0 ± 0.0 23.1 ± 0.3 0.7 ± 0.3 27.8 ± 0.2 22.2 ± 0.3
  Alk. peroxide 0.4 ± 0.1 37.7 ± 0.6 43.8 ± 0.3 0.8 ± 0.3 54.8 ± 2.2 42.3 ± 0.2
DDGS AFEX 0.8 ± 0.1 32.6 ± 0.4 42.6 ± 0.4 1.1 ± 0.7 5.6 ± 0.2 4.9 ± 1.2
  0.25% NaOH 1.2 ± 0.1 26.4 ± 0.2 32.0 ± 0.2 0.8 ± 0.1 3.8 ± 0.8 3.5 ± 0.6
  Alk. peroxide 2.8 ± 0.1 31.5 ± 0.2 38.1 ± 0.3 2.1 ± 0.5 7.3 ± 0.6 6.4 ± 0.8
Poplar AFEX 2.1 ± 0.3 18.0 ± 0.1 17.6 ± 0.0 0.0 ± 0.0 21.3 ± 0.8 21.5 ± 0.3
  0.25% NaOH 0.1 ± 0.1 10.3 ± 0.3 17.1 ± 0.4 0.3 ± 0.0 44.4 ± 0.1 32.3 ± 0.5
  Alk. peroxide 0.8 ± 0.0 17.8 ± 0.2 18.0 ± 0.0 0.1 ± 0.1 43.9 ± 1.4 37.3 ± 0.7
  1. *Glc and Xyl release are expressed as a percentage of total Glc and Xyl in each substrate (Additional file 1, Table S2). Data are expressed as the means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan.