Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Table 1 Commercial enzyme benchmarks for release of Glc and Xyl from 15 pretreatment/feedstock combinations*

Feedstock	Pretreatment	Glc release (%)			Xyl release (%)
		No enzyme	Spezyme CP	Accellerase 1000	No enzyme	Spezyme CP	Accellerase 1000
Corn stover	AFEX	1.0 ± 0.0	44.0 ± 0.0	50.6 ± 0.3	1.2 ± 0.0	34.4 ± 0.5	30.0 ± 0.6
	0.25% NaOH	1.1 ± 0.0	40.7 ± 0.1	54.1 ± 0.3	1.2 ± 1.2	35.4 ± 0.2	22.9 ± 0.3
	Alk. peroxide	0.4 ± 0.0	58.4 ± 0.1	68.6 ± 0.3	0.0 ± 0.0	49.6 ± 0.6	38.0 ± 0.6
Switchgrass	AFEX	2.1 ± 0.0	22.9 ± 0.1	28.1 ± 0.0	0.0 ± 0.0	22.6 ± 0.7	23.4 ± 0.1
	0.25% NaOH	0.6 ± 0.1	16.4 ± 0.1	31.1 ± 0.1	0.0 ± 0.0	30.5 ± 0.2	23.2 ± 0.1
	Alk. peroxide	1.4 ± 0.0	41.6 ± 0.4	50.6 ± 0.1	0.0 ± 0.0	45.1 ± 0.2	34.6 ± 0.3
Miscanthus	AFEX	1.2 ± 0.3	36.2 ± 0.3	35.1 ± 0.0	0.0 ± 0.0	36.9 ± 1.0	36.8 ± 0.5
	0.25% NaOH	0.0 ± 0.0	13.0 ± 0.0	23.1 ± 0.3	0.7 ± 0.3	27.8 ± 0.2	22.2 ± 0.3
	Alk. peroxide	0.4 ± 0.1	37.7 ± 0.6	43.8 ± 0.3	0.8 ± 0.3	54.8 ± 2.2	42.3 ± 0.2
DDGS	AFEX	0.8 ± 0.1	32.6 ± 0.4	42.6 ± 0.4	1.1 ± 0.7	5.6 ± 0.2	4.9 ± 1.2
	0.25% NaOH	1.2 ± 0.1	26.4 ± 0.2	32.0 ± 0.2	0.8 ± 0.1	3.8 ± 0.8	3.5 ± 0.6
	Alk. peroxide	2.8 ± 0.1	31.5 ± 0.2	38.1 ± 0.3	2.1 ± 0.5	7.3 ± 0.6	6.4 ± 0.8
Poplar	AFEX	2.1 ± 0.3	18.0 ± 0.1	17.6 ± 0.0	0.0 ± 0.0	21.3 ± 0.8	21.5 ± 0.3
	0.25% NaOH	0.1 ± 0.1	10.3 ± 0.3	17.1 ± 0.4	0.3 ± 0.0	44.4 ± 0.1	32.3 ± 0.5
	Alk. peroxide	0.8 ± 0.0	17.8 ± 0.2	18.0 ± 0.0	0.1 ± 0.1	43.9 ± 1.4	37.3 ± 0.7

*Glc and Xyl release are expressed as a percentage of total Glc and Xyl in each substrate (Additional file 1, Table S2). Data are expressed as the means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan.