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Table 2 Optimized proportions of six core enzymes for release of Glc from 15 pretreatment/substrate combinations*

From: Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

   Optimized enzyme proportions (%) Glc yield (%)
Feedstock Pre-treatment CBH1 BG EG1 BX EX3 CBH2 MP Exptl.
Corn stover AFEX 35 12 26 4 19 4 42.9 43.9 ± 0.5
  0.25% NaOH 49 5 34 4 4 4 42.0 40.7 ± 1.0
  Alk. peroxide 43 8 30 4 11 4 58.9 58.2 ± 0.2
Switchgrass AFEX 31 4 23 4 35 4 23.0 24.4 ± 0.6
  0.25% NaOH 46 12 30 4 4 4 27.0 25.9 ± 0.8
  Alk. peroxide 47 4 19 4 22 4 38.0 39.1 ± 0.5
Miscanthus AFEX 42 4 42 4 4 4 23.0 22.8 ± 1.3
  0.25% NaOH 36 4 46 4 5 4 17.0 17.5 ± 0.7
  Alk. peroxide 48 4 32 4 7 4 30.0 32.1 ± 1.2
DDGS AFEX 28 20 23 4 20 4 21.3 22.6 ± 0.8
  0.25% NaOH 27 27 34 4 4 4 23.1 23.8 ± 0.5
  Alk. peroxide 16 43 29 4 4 4 31.0 29.8 ± 0.5
Poplar AFEX 44 4 40 4 4 4 14.5 13.8 ± 0.1
  0.25% NaOH 47 4 36 4 4 4 10.0 9.8 ± 0.1
  Alk. peroxide 47 4 37 4 4 4 10.0 10.5 ± 0.2
  1. *MP, model prediction. Exptl., experimental results. Data are expressed as means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan.