Skip to main content

Advertisement

Table 3 Optimized proportions of six core enzymes for release of Xyl from 15 pretreatment/substrate combinations*

From: Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

   Optimized enzyme proportions (%) Xyl yield (%)
Feedstock Pretreatment CBH1 BG EG1 BX EX3 CBH2 MP Exptl.
Corn stover AFEX 7 4 20 26 39 4 28.2 27.8 ± 0.7
  0.25% NaOH 23 4 23 23 23 4 29.0 30.1 ± 0.5
  Alk. peroxide 4 4 30 35 23 4 40.4 40.0 ± 2.0
Switchgrass AFEX 4 4 18 23 47 4 26.2 24.8 ± 0.5
  0.25% NaOH 17 4 23 31 21 4 27.0 26.3 ± 0.6
  Alk. peroxide 4 4 4 37 47 4 40.5 39.5 ± 0.3
Miscanthus AFEX 14 4 20 30 28 4 37.2 35.5 ± 1.0
  0.25% NaOH 20 4 28 25 19 4 22.6 23.0 ± 0.0
  Alk. peroxide 8 4 24 29 31 4 42.3 40.6 ± 0.9
DDGS AFEX - - - - - - - < 7.0
  0.25% NaOH - - - - - - - < 7.0
  Alk. peroxide - - - - - - - < 7.0
Poplar AFEX 4 4 11 28 47 5 19.6 21.0 ± 0.0
  0.25% NaOH 7 4 25 16 43 4 23.0 23.8 ± 1.1
  Alk. peroxide 4 4 23 19 46 4 25.2 26.0 ± 0.5
  1. *MP, model prediction. Exptl., experimental results. Data are expressed as means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan. Xyl release from all DDG/pretreatment combinations was too low to construct statistically significant models.