| | |
Optimized enzyme proportions (%)
|
Xyl yield (%)
|
|---|
|
Feedstock
|
Pretreatment
|
CBH1
|
BG
|
EG1
|
BX
|
EX3
|
CBH2
|
MP
|
Exptl.
|
|
Corn stover
|
AFEX
|
7
|
4
|
20
|
26
|
39
|
4
|
28.2
|
27.8 ± 0.7
|
| |
0.25% NaOH
|
23
|
4
|
23
|
23
|
23
|
4
|
29.0
|
30.1 ± 0.5
|
| |
Alk. peroxide
|
4
|
4
|
30
|
35
|
23
|
4
|
40.4
|
40.0 ± 2.0
|
|
Switchgrass
|
AFEX
|
4
|
4
|
18
|
23
|
47
|
4
|
26.2
|
24.8 ± 0.5
|
| |
0.25% NaOH
|
17
|
4
|
23
|
31
|
21
|
4
|
27.0
|
26.3 ± 0.6
|
| |
Alk. peroxide
|
4
|
4
|
4
|
37
|
47
|
4
|
40.5
|
39.5 ± 0.3
|
|
Miscanthus
|
AFEX
|
14
|
4
|
20
|
30
|
28
|
4
|
37.2
|
35.5 ± 1.0
|
| |
0.25% NaOH
|
20
|
4
|
28
|
25
|
19
|
4
|
22.6
|
23.0 ± 0.0
|
| |
Alk. peroxide
|
8
|
4
|
24
|
29
|
31
|
4
|
42.3
|
40.6 ± 0.9
|
|
DDGS
|
AFEX
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
< 7.0
|
| |
0.25% NaOH
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
< 7.0
|
| |
Alk. peroxide
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
< 7.0
|
|
Poplar
|
AFEX
|
4
|
4
|
11
|
28
|
47
|
5
|
19.6
|
21.0 ± 0.0
|
| |
0.25% NaOH
|
7
|
4
|
25
|
16
|
43
|
4
|
23.0
|
23.8 ± 1.1
|
| |
Alk. peroxide
|
4
|
4
|
23
|
19
|
46
|
4
|
25.2
|
26.0 ± 0.5
|
- *MP, model prediction. Exptl., experimental results. Data are expressed as means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan. Xyl release from all DDG/pretreatment combinations was too low to construct statistically significant models.
