Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Table 3 Optimized proportions of six core enzymes for release of Xyl from 15 pretreatment/substrate combinations*

		Optimized enzyme proportions (%)						Xyl yield (%)
Feedstock	Pretreatment	CBH1	BG	EG1	BX	EX3	CBH2	MP	Exptl.
Corn stover	AFEX	7	4	20	26	39	4	28.2	27.8 ± 0.7
	0.25% NaOH	23	4	23	23	23	4	29.0	30.1 ± 0.5
	Alk. peroxide	4	4	30	35	23	4	40.4	40.0 ± 2.0
Switchgrass	AFEX	4	4	18	23	47	4	26.2	24.8 ± 0.5
	0.25% NaOH	17	4	23	31	21	4	27.0	26.3 ± 0.6
	Alk. peroxide	4	4	4	37	47	4	40.5	39.5 ± 0.3
Miscanthus	AFEX	14	4	20	30	28	4	37.2	35.5 ± 1.0
	0.25% NaOH	20	4	28	25	19	4	22.6	23.0 ± 0.0
	Alk. peroxide	8	4	24	29	31	4	42.3	40.6 ± 0.9
DDGS	AFEX	-	-	-	-	-	-	-	< 7.0
	0.25% NaOH	-	-	-	-	-	-	-	< 7.0
	Alk. peroxide	-	-	-	-	-	-	-	< 7.0
Poplar	AFEX	4	4	11	28	47	5	19.6	21.0 ± 0.0
	0.25% NaOH	7	4	25	16	43	4	23.0	23.8 ± 1.1
	Alk. peroxide	4	4	23	19	46	4	25.2	26.0 ± 0.5

*MP, model prediction. Exptl., experimental results. Data are expressed as means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan. Xyl release from all DDG/pretreatment combinations was too low to construct statistically significant models.