Table 3 Optimized proportions of six core enzymes for release of Xyl from 15 pretreatment/substrate combinations*

Feedstock

Pretreatment

CBH1

BG

EG1

BX

EX3

CBH2

MP

Exptl.

Corn stover

AFEX

7

4

20

26

39

4

28.2

27.8 ± 0.7

0.25% NaOH

23

4

23

23

23

4

29.0

30.1 ± 0.5

Alk. peroxide

4

4

30

35

23

4

40.4

40.0 ± 2.0

Switchgrass

AFEX

4

4

18

23

47

4

26.2

24.8 ± 0.5

0.25% NaOH

17

4

23

31

21

4

27.0

26.3 ± 0.6

Alk. peroxide

4

4

4

37

47

4

40.5

39.5 ± 0.3

Miscanthus

AFEX

14

4

20

30

28

4

37.2

35.5 ± 1.0

0.25% NaOH

20

4

28

25

19

4

22.6

23.0 ± 0.0

Alk. peroxide

8

4

24

29

31

4

42.3

40.6 ± 0.9

DDGS

AFEX

-

-

-

-

-

-

-

< 7.0

0.25% NaOH

-

-

-

-

-

-

-

< 7.0

Alk. peroxide

-

-

-

-

-

-

-

< 7.0

Poplar

AFEX

4

4

11

28

47

5

19.6

21.0 ± 0.0

0.25% NaOH

7

4

25

16

43

4

23.0

23.8 ± 1.1

Alk. peroxide

4

4

23

19

46

4

25.2

26.0 ± 0.5